Author(s): Tuti Kusumaningsih, Ira Arundina, Tantiana, Rini Devijanti R, Indeswati Diyatri, Mohammed Aljunaid, Poppy Raissa Hudayanti

Email(s): tuti-k@fkg.unair.ac.id , tutikusumaningsih@yahoo.com

DOI: 10.52711/0974-360X.2021.00538   

Address: Tuti Kusumaningsih1*, Ira Arundina1, Tantiana1, Rini Devijanti R1, Indeswati Diyatri1, Mohammed Aljunaid2, Poppy Raissa Hudayanti3
1Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga Surabaya- Indonesia.
2Postgraduate Program of Oral and Dental Health Science, Faculty of Dental Medicine, Universitas Airlangga, Surabaya- Indonesia.
3Under Graduate Student of Faculty of Dental Medicine, Universitas Airlangga Surabaya- Indonesia.
*Corresponding Author

Published In:   Volume - 14,      Issue - 6,     Year - 2021


ABSTRACT:
Background: Periodontitis is an inflammatory disease of the periodontal tissues usually provoked by Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) bacteria. In the periodontitis healing process, macrophages play a significant role in the inflammatory stage. Brotowali (Tinospora crispa) consists of flavonoids that can stimulate periodontitis healing. Methods: Brotowali extract was derived from dried brotowali stems, processed into powder, and macerated with ethanol 80%. After that, the extraction was performed in three different concentrations, which were 25%, 50%, and 100%. five groups of Wistar rats were divided into Healthy wistar rats (control negative) Wistar rats suffering from periodontitis (control positive), Wistar rats suffering from periodontitis treated with 0.1 ml of 25% brotowali extract (treatment I), suffering from periodontitis treated with 0.1 ml of 50% brotowali extract (treatment II), and Wistar rats suffering from periodontitis treated with 0.1 ml of 100% brotowali extract (treatment III). The histological slide of each sample then was made to determine the number of macrophages cells. Results: One-Way ANOVA analysis revealed a notable difference among the groups. Tukey HSD Test (Post Hoc Test) showed no significant difference between (treatment group I) and (treatment group II). Conclusion: Brotowali extract may influence macrophage cells count of Wistar rats caused by A. actinomycetemcomitans bacteria.


Cite this article:
Tuti Kusumaningsih, Ira Arundina, Tantiana, Rini Devijanti R, Indeswati Diyatri, Mohammed Aljunaid, Poppy Raissa Hudayanti. The Effects of Brotowali (Tinospora crispa) Extract on The Number of Macrophage Cells in Wistar Rats (Rattus norvegicus) Suffering from Periodontitis. Research Journal of Pharmacy and Technology. 2021; 14(6):3077-1. doi: 10.52711/0974-360X.2021.00538

Cite(Electronic):
Tuti Kusumaningsih, Ira Arundina, Tantiana, Rini Devijanti R, Indeswati Diyatri, Mohammed Aljunaid, Poppy Raissa Hudayanti. The Effects of Brotowali (Tinospora crispa) Extract on The Number of Macrophage Cells in Wistar Rats (Rattus norvegicus) Suffering from Periodontitis. Research Journal of Pharmacy and Technology. 2021; 14(6):3077-1. doi: 10.52711/0974-360X.2021.00538   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-6-28


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