The aim of present work is to evaluate the laxative, anti-diarrhoeal and anti-emetic activity extract of Citrus limon by using different solvents like ethanol and ethyl-acetate. The methods employed are measuring fecal output in naive rats, loperamide induced constipation in rats, castor oil induced diarrhoea in rats and anti-emetic activity was evaluated using chick model. In fecal output in naive rats, the consistency of stools expelled within 24hrs after administration of extracts was observed. The loperamide induced constipation in rats using standard drug sodium picosulphate (5mg/kg), the faeces were collected after 8hrs and stool consistency was observed. At higher dose there is increase in fecal matter and compared with standard. The castor oil induced diarrhoea decreased total no. of feces was observed after 4hrs and these values are compared with control. In anti-emetic activity, the chicks after 10 minutes of copper sulphate pentahydrate(50mg/kg) administration there was an increase in no. of retchings (An emetic action without emeting gastric material) and the results are comparable with that of standard Chlorpromazine(150mg/kg).
Cite this article:
K. Ravishankar, K. Sudharani. Comparative in-vivo Study of Laxative, Anti-diarrheoal and Anti-emetic activity of Ethanolic and Ethyl Acetate Leaf Extract of Citrus limon in Rats and Chicks. Research Journal of Pharmacy and Technology. 2021; 14(6):3045-0. doi: 10.52711/0974-360X.2021.00532
K. Ravishankar, K. Sudharani. Comparative in-vivo Study of Laxative, Anti-diarrheoal and Anti-emetic activity of Ethanolic and Ethyl Acetate Leaf Extract of Citrus limon in Rats and Chicks. Research Journal of Pharmacy and Technology. 2021; 14(6):3045-0. doi: 10.52711/0974-360X.2021.00532 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-6-22
1. Akuodor GC, Muazzam I, Usman-Idris M, Megwas UA, Akpan JL, Chilaka KC, Okoroafor DO, Osunkwo UA. Evaluation of the antidiarrheal activity of methanol leaf extract of Bombax b
2. Saito T, Mizutani F, Iwanaga Y, Morikawa K, Kato H. Laxative and anti-diarrheal activity of polycarbophil in mice and rats. Japanese Journal of Pharmacology. 2002 Jan 1;89(2): 133-41. uonopozense in rats. Ibnosina J Med BS. 2011 Jan 1;3(1): 15-20.
3. Mohtasheemul HM, Salman A, Ziauddin A, Iqbal A. Antiemetic Activity of Some aromatic plants. JPSI. 2012;1(1): 47-9.
4. Sujitha MV, Kannan S. Green synthesis of gold nanoparticles using Citrus fruits (Citrus limon, Citrus reticulata and Citrus sinensis) aqueous extract and its characterization. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2013 Feb 1; 102: 15-23.
5. Ewansiha JU, Garba SA, Musa G, Daniyan SY, Busari MB, Innalegwu DA, Doughari JH. Preliminary phytochemical and Antimicrobial Activity of Citrus x limon (L) Burm. f.(lemon) leaf extract against some pathogenic microorganisms. Journal of Applied Life Sciences International. 2016 Jul 31: 1-0.
6. Organization for Economic Cooperation and Development (OECD), Guideline 420. Acute Oral Toxicity—Fixed Dose Procedure, 2001
7. Takaharu Saito, Fujie Mizutani, Yuji Iwanaga, Kouji Morikawa and Hideo Kato. Laxative and Anti-diarrheal Activity of Polycarbophil in Mice and Rats. JPN. J. Pharmacol. 89, 133 – 141 (2002)
8. Méité S, Bahi C, Yéo D, Datté JY, Djaman JA, N'guessan DJ. Laxative activities of Mareya micrantha (Benth.) Müll. Arg. (Euphorbiaceae) leaf aqueous extract in rats. BMC Complementary and Alternative Medicine. 2010 Dec;10(1): 7.
9. Akita Y, Yang Y, Kawai T, Kinoshita K, Koyama K, Takahashi K, et al. New assay method for surveying anti-emetic compounds from natural sources. Natural Product Sciences. 1998;4(2): 72-7.
10. Iwao I, Terada Y. On the mechanism of diarrhea due to castor oil. Jpn J Pharmacol 1962; 12: 137-45.
11. Kojima R, Doihara H, Nozawa K, Kawabata-Shoda E, Yokoyama T, Ito H. Characterization of two models of drug-induced constipation in mice and evaluation of mustard oil in these models. Pharmacology. 2009;84(4): 227–33.
12. Kim JE, Yun WB, Sung JE, Lee HA, Choi JY, Choi YS, et al. Characterization the response of Korl: ICR mice to loperamide induced constipation. Lab Anim Res. 2016;32(4): 231–40
13. Cirillo C, Capasso R. Constipation and Botanical Medicines: An Overview. Phytother Res. 2015;29(10): 1488–93.
14. Choi JS, Kim JW, Kim KY, Lee JK, Sohn JH, Ku SK. Synergistic effect of fermented rice extracts on the probiotic and laxative properties of yoghurt in rats with loperamide-induced constipation. Evid Based Complement Alternat Med. 2014; 2014: 878503.
15. Harada Y, Iizuka S, Saegusa Y, Mogami S, Fujitsuka N, Hattori T. Mashiningan Improves Opioid-Induced Constipation in Rats by Activating Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel. J Pharmacol Exp Ther. 2017;362(1): 78–84. Epub 2017/05/04. pmid: 28465373.
16. Chokhavatia S, John ES, Bridgeman MB, Dixit D. Constipation in Elderly Patients with Noncancer Pain: Focus on Opioid-Induced Constipation. Drugs Aging. 2016;33(8): 557–74.
17. Kim JE, Go J, Koh EK, Song SH, Sung JE, Lee HA, et al. Gallotannin-Enriched Extract Isolated from Galla Rhois May Be a Functional Candidate with Laxative Effects for Treatment of Loperamide-Induced Constipation of SD Rats. PLoS One. 2016;11(9): e0161144
18. Sabiu S, Ashafa OT. Toxicological implications and laxative potential of ethanol root extract of Morella serrata in loperamide-induced constipated Wistar rats. Pharm Biol. 2016;54(12): 2901–8
19. Wang S, Borison HL. Copper sulphate emesis: A study of afferent pathways from the gastrointestinal tract. Am J Physiol Legacy Content. 1951; 164: 520-26.
20. Horn CC. The Physiology of vomiting, nausea and vomiting. Springer, 2017, pp. 15-25.
21. Hussain M, Raza SM, Janbaz KH. Pharmacologically mechanistic basis for the traditional uses of Rumex acetosa in gut motility disorders and emesis. Bangladesh J Pharmacol. 2015; 10: 548-54.