Author(s): Jai Bharti Sharma, Sherry, Shailendra Bhatt, Vipin Saini, Manish Kumar

Email(s): shailu.bhatt@gmail.com

DOI: 10.52711/0974-360X.2021.00520   

Address: Jai Bharti Sharma1, Sherry1, Shailendra Bhatt1*, Vipin Saini2, Manish Kumar1
1MM College of Pharmacy, MM (Deemed to be) University, Mullana, Ambala, Haryana, India.
2Maharishi Markandeshwar University, Solan, HP, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 6,     Year - 2021


ABSTRACT:
Background: Due to solubility issues of curcumin and tetrahydrocurcumin, there is a need for the development of a UV-Visible spectrophotometric method that can estimate the drug release precisely and accurately. The addition of surfactant in the dissolution medium in low concentration achieved bio-comparable surface activity and can be used to estimate the drug release from formulations by avoiding sink conditions. Objective: The purpose of the present investigation was to develop a simple and précise UV-Visible spectrophotometric method for the determination of curcumin and tetrahydrocurcumin after oral administration. Method: A UV-Visible spectrophotometric method was developed using an appropriate solvent system for the estimation of curcumin and tetrahydrocurcumin. The solvent system having simulated intestinal fluid and particular concentration of surfactant was selected and further validated according to guidelines of the international conference on harmonization (ICH), the analytical parameter like linearity, precision and accuracy, etc. were studied. Results: Simulated intestinal fluid pH 7.4 with tween 80 at 1 % concentration satisfied all the conditions relative to peak quality at the stated wavelength for curcumin and intestinal fluid pH 7.4 with tween 80 at 0.5% concentration satisfied all the conditions relative to Peak quality at the stated wavelength for tetrahydrocurcumin. The developed methods were found within the range of all the validation parameters. Conclusion: The proposed method was found to be very simple and precise and can be used for routine quantitative analysis of curcumin and tetrahydrocurcumin.


Cite this article:
Jai Bharti Sharma, Sherry, Shailendra Bhatt, Vipin Saini, Manish Kumar. Development and Validation of UV-Visible Spectrophotometric method for the Estimation of Curcumin and Tetrahydrocurcumin in Simulated Intestinal Fluid. Research Journal of Pharmacy and Technology. 2021; 14(6):2971-5. doi: 10.52711/0974-360X.2021.00520

Cite(Electronic):
Jai Bharti Sharma, Sherry, Shailendra Bhatt, Vipin Saini, Manish Kumar. Development and Validation of UV-Visible Spectrophotometric method for the Estimation of Curcumin and Tetrahydrocurcumin in Simulated Intestinal Fluid. Research Journal of Pharmacy and Technology. 2021; 14(6):2971-5. doi: 10.52711/0974-360X.2021.00520   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-6-10


REFERENCES:
1.    Chirio D, Peira E, Dianzani C, Muntoni E, Gigliotti CL, Ferrara B, et al. Development of solid lipid nanoparticles by cold dilution of microemulsions: Curcumin loading, preliminary in vitro Studies, and biodistribution. J. Nanomater., 2019; 9(2): 230.
2.    Jourghanian P, Ghaffari S, Ardjmand M, Haghighat S, Mohammadnejad M. Sustained release curcumin loaded solid lipid nanoparticles. Adv. Pharm. Bull., 2016 ; 6(1): 17.
3.    Fadus MC, Lau C, Bikhchandani J, Lynch HT. Curcumin: An age-old anti-inflammatory and anti-neoplastic agent. JTCM., 2017; 7(3): 339-46.
4.    Sharma JB, Bhatt S, Sharma A, Kumar M. Recent Advances in Curcumin Nanocarriers for the Treatment of Different Types of Cancer with Special Emphasis on In Vitro Cytotoxicity and Cellular Uptake Studies. Nanosci. and Nanotechnol-Asia., 2020; 10(5): 577-90.
5.    Bhatt H, Rompicharla SV, Komanduri N, Aashma S, Paradkar S, Ghosh B, et al. Development of curcumin-loaded solid lipid nanoparticles utilizing glyceryl Monostearate as single lipid using QbD approach: characterization and evaluation of anticancer activity against human breast cancer cell line. Curr. Drug deliv., 2018; 15(9): 1271-83.
6.    Minafra L, Porcino N, Bravatà V, Gaglio D, Bonanomi M, Amore E, et al. Radiosensitizing effect of curcumin-loaded lipid nanoparticles in breast cancer cells. Sci. Rep., 2019; 9(1): 1-6.
7.    Kalaycıoğlu Z, Gazioğlu I, Erim FB. Comparison of antioxidant, anticholinesterase, and antidiabetic activities of three curcuminoids isolated from Curcuma longa L. Erim, Nat prod res., 2017; 31(24): 2914-7.
8.    Kimura S, Kiriyama A, Araki K, Yoshizumi M, Enomura M, Inoue D, et al. Novel strategy for improving the bioavailability of curcumin based on a new membrane transport mechanism that directly involves solid particles. Eur J Pharm and Biopharm., 2018; 122: 1-5.
9.    Rapalli VK, Kaul V, Gorantla S, Waghule T, Dubey SK, Pandey MM, et al. UV Spectrophotometric method for characterization of curcumin loaded nanostructured lipid nanocarriers in simulated conditions: method development, in-vitro and ex-vivo applications in topical delivery. Spectrochimica Acta A,. 2020; 224: 117392.
10.    Holder GM, Plummer JL, Ryan AJ. The metabolism and excretion of curcumin (1, 7-bis-(4-hydroxy-3-methoxyphenyl)-1, 6-heptadiene-3, 5-dione) in the rat. Xenobiotica. 1978; 8(12): 761-8.
11.    Mahal A, Wu P, Jiang ZH, Wei X. Schiff bases of tetrahydrocurcumin as potential anticancer agents. Chemistry Select., 2019; 4(1): 366-9.
12.    Xi J, Luo X, Wang Y, Li J, Guo L, Wu G, Li Q. Tetrahydrocurcumin protects against spinal cord injury and inhibits the oxidative stress response by regulating FOXO4 in model rats. Exp. Ther. Med., 2019; 18(5): 3681-7.
13.    Murugan P, Pari L. Influence of tetrahydrocurcumin on erythrocyte membrane bound enzymes and antioxidant status in experimental type 2 diabetic rats. J. Ethnopharmacol., 2007; 113(3): 479-86.
14.    Pari L, Murugan P. Changes in glycoprotein components in streptozotocin-nicotinamide induced type 2 diabetes: influence of tetrahydrocurcumin from Curcuma longa. Plant Food Hum. Nut., 2007 1; 62(1): 25-9.
15.    Aggarwal BB, Deb L, Prasad S. Curcumin differs from tetrahydrocurcumin for molecular targets, signaling pathways and cellular responses. Molecules. 2015; 20(1): 185-205.
16.    Shin MS, Yu JS, Lee J, Ji YS, Joung HJ, Han YM, Yoo HH, Kang KS. A Hydroxypropyl Methylcellulose-Based Solid Dispersion of Curcumin with Enhanced Bioavailability and its Hepatoprotective Activity. Biomolecules. 2019; 9(7): 281.
17.    He P, Yan H, Zhao J, Gou M, Li X. An evaluation of the wound healing potential of tetrahydrocurcumin-loaded MPEG-PLA nanoparticles. J. Biomater. Appl., 2019; 34(3): 315-25.
18.    Indulkar AS, Gao Y, Raina SA, Zhang GG, Taylor LS. Crystallization from supersaturated solutions: role of lecithin and composite simulated intestinal fluid. Pharm. Res., 2018; 35(8):158.
19.    ICH –topic Q2 ; Validation of analytical methods ; The European agency for the evaluation of medicinal products. Human medicines evaluation unit , CPMP/ICH/381/95.
20.    Filist M, Szlaska I, Kaza M, Pawiński T. Validated HPLC‐UV method for determination of naproxen in human plasma with proven selectivity against ibuprofen and paracetamol. Biomed. Chromatogr., 2016 Jun; 30(6): 953-61.
21.    e Silva AF, de Castro WV, de Andrade FP. Development of spectrophotometric method for iron determination in fortified wheat and maize flours. Food Chemi., 2018; 242: 205-10.
22.    Tang Y, Fields C. A UHPLC-UV Method development and validation for determining kavalactones and flavokavains in Piper methysticum (Kava). Molecules. 2019; 24(7): 1245.
23.    Iammarino M, Mentana A, Centonze D, Palermo C, Mangiacotti M, Chiaravalle AE. Simultaneous determination of twelve dyes in meat products: Development and validation of an analytical method based on HPLC-UV-diode array detection. Food Chemi., 2019; 285: 1-9.
24.    Al-Rimawi F. Development and validation of an analytical method for metformin hydrochloride and its related compound (1-cyanoguanidine) in tablet formulations by HPLC-UV. Talanta. 2009;79(5): 1368-71.
25.    Rahman SM, Telny TC, Ravi TK, Kuppusamy S. Role of surfactant and pH in dissolution of curcumin. Indian J. Pharma. Sci., 2009;71(2): 139.
26.    Trivedi MK, Panda P, Sethi KK, Gangwar M, Mondal SC, Jana S. Solid and liquid state characterization of tetrahydrocurcumin using XRPD, FT-IR, DSC, TGA, LC-MS, GC-MS, and NMR and its biological activities. Journal of Pharmaceutical Analysis. 2020;10(4): 334-45.

Recomonded Articles:

Author(s): Nimbalkar V.V., Pansare P.M., Nishane B.B.

DOI: 10.5958/0974-360X.2015.00310.8         Access: Open Access Read More

Author(s): Trophimus Gnanabagyan Jayakaran, Vignesh R, Shankar P

DOI: 10.5958/0974-360X.2019.00700.5         Access: Open Access Read More

Author(s): Vijayalakshmi. S , Disalva. X, Chittaranjan Srivastava, Arun A

DOI: 10.5958/0974-360X.2019.00520.1         Access: Open Access Read More

Author(s): Pande S. D., Wagh A.S., Bhagure L.B., Patil S.G., Deshmukh A.R.

DOI: 10.5958/0974-360X.2015.00070.0         Access: Open Access Read More

Author(s): Deepak Karki, Gururaj S. Kulkarni, Shivakumar Swamy, Sheeba FR

DOI: 10.5958/0974-360X.2017.00750.8         Access: Open Access Read More

Author(s): Vandana Devi Sahu, Mukta Agrawal, Mukesh Sharma, Sandhya Chandrakar, Sujata Gupta, Garima Sharma, Kalyani Dewangan, Harsha Solanki, Manisha Majumdar, D. K. Tripathi, Amit Alexander, Ajazuddin

DOI: 10.5958/0974-360X.2016.00145.1         Access: Open Access Read More

Author(s): Muhammad Hamdan, Noorhamdani AS, Masruroh Rahayu, Mohammmad Hasan Machfoed

DOI: 10.5958/0974-360X.2019.01020.5         Access: Open Access Read More

Author(s): Loveleen Preet Kaur, Rajeev Garg, GD Gupta

DOI: Not Available         Access: Open Access Read More

Author(s): Vaseeha Banu T.S., Sandhya K.V., K.N. Jayaveera

DOI: Not Available         Access: Open Access Read More

Author(s): Y. Prem Kumar, K. Vinod Kumar, V. Sai Kishore

DOI: Not Available         Access: Open Access Read More

Author(s): M. Sumithra, Avantika Prabhabanik

DOI: 10.5958/0974-360X.2017.00036.1         Access: Open Access Read More

Author(s): V Rajesh Babu, Syeda Rana Nikhat, P Nivethithai, SH Areefulla

DOI: Not Available         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags