Shyamala Nayak, Nayanatara Arun Kumar, Anupama Hegde, Rekha D Kini, Vandana Blossom, Roopesh Poojary
Shyamala Nayak1, Nayanatara Arun Kumar2*, Anupama Hegde1, Rekha D Kini2, Vandana Blossom3, Roopesh Poojary2
1Department of Biochemistry, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal.
2Department of Physiology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal.
3Department of Anatomy, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal.
Volume - 14,
Issue - 5,
Year - 2021
Natural products are gaining much importance in light of the serious side effects posed by drugs of chemical origin. High intake of foods rich in antioxidants reduces the risk of neurodegenerative disorders. Traditionally, Allium sativum L.(garlic) and Allium cepa. L (onion) has received considerable attention for their therapeutic benefits around the globe. The present study assesses the free radical scavenging role of Allium sativum and Allium cepa in cerebral cortex, striatum, and hippocampus. Adult wistar rats of either sex were grouped as control group (Group I) treated with normal saline and the two experimental group were treated with the aqueous bulbous extracts of dehydrated Allium sativum (Group II) and Allium cepa (Group III) was considered as treated groups. Homogenates of hippocampus, striatum and cerebral cortex were analyzed for biochemical and neuronal analysis. LD50 value of these extracts in rats was found at a dose of 500?mg/kg BW. A Significant decline (P<0.05) in the MDA level was observed in the hippocampus, striatum and cerebral cortex in group II when compared to group III. Total antioxidant level, GSH, SOD level was significantly high (P<0.001) in the treated groups. Neuronal increase was significant in Group II (P < 0.01) when compared to Group III. Allium sativum and Allium cepa was found to have a challenging role in hampering oxidative stress in Hippocampus, striatum and cerebral cortex, the target regions in neurological disorders. However, ample number of studies are required to establish their mechanism of action as a progression to clinical approach.
Cite this article:
Shyamala Nayak, Nayanatara Arun Kumar, Anupama Hegde, Rekha D Kini, Vandana Blossom, Roopesh Poojary. Neuroprotective role of Allium cepa and Allium sativum on Hippocampus, striatum and Cerebral cortex in Wistar rats. Research Journal of Pharmacy and Technology. 2021; 14(5):2406-1. doi: 10.52711/0974-360X.2021.00424
Shyamala Nayak, Nayanatara Arun Kumar, Anupama Hegde, Rekha D Kini, Vandana Blossom, Roopesh Poojary. Neuroprotective role of Allium cepa and Allium sativum on Hippocampus, striatum and Cerebral cortex in Wistar rats. Research Journal of Pharmacy and Technology. 2021; 14(5):2406-1. doi: 10.52711/0974-360X.2021.00424 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-5-8
1. Gitler AD, Dhillon P, Shorter J. Neurodegenerative disease: models, mechanisms, and a new hope. Dis Model Mech. 2017;10(5): 499-502.
2. Malarkodi Velraj, N. Lavaniya. Alzheimer Disease and a Potential Role of Herbs-A Review. Research J. Pharm. and Tech 2018; 11(6): 2695-2700.
3. Hagan KA, Munger KL, Ascherio A, Grodstein F. Epidemiology of major neurodegenerative diseases in women: Contribution of the nurses’ health study. Am J Public Health.2016; 106(9): 1650-1655.
4. Uttara B, Singh AV, Zamboni P, Mahajan RT. Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol.2009; 7(1): 65-74.
5. Chen X, Guo C, Kong J. Oxidative stress in neurodegenerative diseases. Neural Regen Res 2012;( 7): 376-85.
6. Vihar Gadhvi, Kachariya Brijesh, Amit Gupta, Komal Roopchandani, Nirav Patel. Nanoparticles for Brain Targeting. Research J. Pharm. and Tech.2013; 6(5): 454-458.
7. Daryoush Fatehi, Ardeshir Moayeri, Omid Rostamzadeh, Ayoob Rostamzadeh, Maziar Malekzadeh Kebria. Reactive Oxygenated Species (ROS) in Male Fertility; Source, Interaction Mechanism and Antioxidant Therapy. Research J. Pharm. and Tech 2018; 11(2): 791-796.
8. Kumar GP, Khanum F. Neuroprotective potential of phytochemicals. Pharmacogn Rev.2012;6(12): 81-90.
9. J.S. Vaghela, S.S. Sisodia. In Vitro Antioxidant Activity of Terminalia chebula Fruit Extracts. Research J. Pharm. and Tech. 2011; 4(12): 1835-1843.
10. Hussein J. Hussein, Imad Hadi Hameed, Mohammed Yahya Hadi. A Review: Anti-microbial, Anti-inflammatory effect and Cardiovascular effects of Garlic: Allium sativum. Research J. Pharm. and Tech 2017; 10(11): 4069-4078.
11. P. Parvathi.Garlic- A Golden Wonder. Research J. Pharm. and Tech. 2018; 11(1): 393-396.
12. Arreola R, Quintero-Fabián S, Lopez-Roa R I, Flores-Gutierrez E O, Reyes-Grajeda J P, Carrera-Quintanar L, Ortuno-Sahagun D. Immunomodulation and anti-inflammatory effects of garlic compounds. J Immunol Res. 2015; 1-13.
13. Nikeherpianti Lolok, Harlyanti Muthmainnah Mashar, Itma Annah, Ahmad Saleh, Wa Ode Yuliastri, Muhammad Isrul. Antidiabetic Effect of the Combination of Garlic Peel Extract (Allium sativum) and Onion Peel (Allium cepa) in Rats with Oral-Glucose Tolerance Method. Research J. Pharm. and Tech. 2019; 12(5): 2153-2156.
14. Ouyang H, Hou K, Peng W, Liu Z, Deng H.Antioxidant and xanthine oxidase inhibitory activities of total polyphenols from onion. Saudi J Biol Sci. 2018;25(7): 1509-1513.
15. Padmini R, Sitrarasi R, Razia M. Molecular Docking Studies of Bioactive Compounds from Allium sativum Against EML4-ALK Receptor. Research J. Pharm. and Tech 2017; 10(11): 3741-3747.
16. Glowinski J, Iversen LL. Regional studies of catecholamines in the rat brain-I. The disposition of [3H] norepinephrine, 3[H]dopamine and 3[H] dopa in various regions of the brain. J Neurochem. 1966; 13: 655-669.
17. Rao GM, Rao AV, Raja A, Rao S, Rao A. Lipid peroxidation in brain tumours. Clin Chim Acta. 2000;302(1-2):205-211.
18. Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol. 2001; 54(5): 356-361.
19. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82(1): 70-77.
20. Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974;47(3): 469-474.
21. Madhyastha S, Somayaji SN, Rao MS, Nalini K, Bairy KL. Hippocampal brain amines in methotrexate-induced learning and memory deficit. Can J Physiol Pharmacol. 2002 (80): 1076-84.
22. Rajesh Kumar Reddy P, Saravanan J and Praveen T K. Evaluation of Neuroprotective Activity of Melissa officinalis in MPTP Model of Parkinson’s Disease in Mice. Research J. Pharm. and Tech. 2019; 12(5): 2103-2108.
23. Soumi Acharya, S. S. Meenambiga. Nanotechnology in Parkinson's Disease - A Review. Research J. Pharm. and Tech. 2020; 13(4): 1965-1969.
24. Subamalani S, Sasikumar A, Vijayaragavan R, Senthilkumar S, Madhan Kumar S, Makesh Raj LS, Kannan I. Effect of Acorus calamus Linn on histomorphometric changes in the CA1 and CA3 regions of Hippocampus in Wistar Albino rats. Research J. Pharm. and Tech. 2019; 12(7): 3531-3536.
25. R. Shasmitha, S. Saravana Kumar. Dose dependent effect of Bacopa monnieri on Stress included Neural Degeneration in CA-1 and CA-3 Hippocampus Region of rat. Research J. Pharm. and Tech. 2019; 12(5): 2353-2355.