Inbathamizh L, Kalpana V, Revathi Yadav K
Inbathamizh L1*, Kalpana V 2, Revathi Yadav K3
1Assistant Professor, Department of Biotechnology, School of Bio and Chemical Engineering,
Sathyabama Institute of Science and Technology, Chennai- 600119, Tamil Nadu, India.
2M.Sc. Student, Department of Biochemistry, Meenakshi College for Women, Chennai-600024, Tamil Nadu, India.
3B.Tech Student, Department of Biotechnology, School of Bio and Chemical Engineering,
Sathyabama Institute of Science and Technology, Chennai- 600119, Tamil Nadu, India.
Volume - 14,
Issue - 5,
Year - 2021
With the increase in the potential applications of nanoparticles in pharma and various fields, nanoparticle research is attracting more attention. Though several chemical and physical methods are being used for the synthesis of metal nanoparticles, they are associated with several disadvantages. Couroupita guianensis is a traditional plant with medicinal values. The focus of the study was to follow a green chemistry route to synthesize silver nanoparticles (AgNPs) using the leaf extract of Couroupita guianensis as a reductant and stabilizing agent. The boiled aqueous leaf extract with silver nitrate solution on exposure to sunlight showed the maximum absorbance at 430nm indicating the synthesis of AgNPs. Ultra Violet (UV)-Visible spectroscopy, Scanning Electron Microscopy (SEM), and Fourier Transform Infrared spectroscopy (FTIR) techniques were used for the characterization of AgNPs. The synthesized AgNPs were found to be spherical and 4.44 – 40.20nm in size. They also seemed to be capped with the significant functional groups present in the leaf extract. Thus, the study suggested Couroupita guianensis mediated green synthesis of AgNPs as an efficient and eco-friendly approach with substantial advantages over the conventional methods. The process could be further scaled-up for mass production and wider applications of AgNPs.
Cite this article:
Inbathamizh L, Kalpana V , Revathi Yadav K. Synthesis and Characterization of Silver Nanoparticles from Couroupita guianensis leaf extract–A Simple Green Route. Research Journal of Pharmacy and Technology. 2021; 14(5):2796-0. doi: 10.52711/0974-360X.2021.00493
Inbathamizh L, Kalpana V , Revathi Yadav K. Synthesis and Characterization of Silver Nanoparticles from Couroupita guianensis leaf extract–A Simple Green Route. Research Journal of Pharmacy and Technology. 2021; 14(5):2796-0. doi: 10.52711/0974-360X.2021.00493 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-5-77
1. Evanoff D, Chumanov G. Size-Controlled Synthesis of Nanoparticles. 2. Measurement of Extinction, Scattering, and Absorption Cross Sections. The Journal of Physical Chemistry B. 2004;108(37):13957-13962.
2. Henglein A. Small-particle research: physicochemical properties of extremely small colloidal metal and semiconductor particles. Chemical Reviews. 1989;89(8):1861-1873.
3. Ahuja J, Gupta U. Magneto convection of rotating nanofluids in porous medium: metals and semiconductors as nanoparticles. Research Journal of Science and Technology. 2017;9(1):135-142.
4. Vedavathi V, Balamurugan K, Dharmaiah G. Analysis of Heat and Mass Transfer on MHD flow with Ag, Al2O3 and Cu Water Nanofluids over a Semi Infinite Surface. Research Journal of Science and Technology. 2017;9(3):359-367.
5. Kumaresn K, Parthiban D, Sivanarayan V, Arun N, Kumaravel P. Toxicity Effect of Copper oxide Nanoparticles on Artemia salina. Research Journal of Pharmacology and Pharmacodynamics. 2015;7(2):53-60.
6. Bhattacharya R, Mukherjee P. Biological properties of “naked” metal nanoparticles. Advanced Drug Delivery Reviews. 2008;60(11):1289-1306.
7. Hada NK, Ashawat MS. Aquasome: A Self-Assembling Supramolecular And Nanoparticulate Carrier System For Bio-Actives. Research Journal Pharmaceutical Dosage Forms and Technology. 2014;6(1):50-53.
8. Namasivayam S, Gnanendra E, Reepika R. Synthesis of silver nanoparticles by Lactobaciluus acidophilus 01 strain and evaluation of its in vitro genomic DNA toxicity. Nano-Micro Letters. 2010;2(3):160-163.
9. Mukherjee S, Chowdhury D, Kotcherlakota R, Patra S, Vinothkumar B et al. Potential Theranostics Application of Bio-Synthesized Silver Nanoparticles (4-in-1 System). Theranostics. 2014;4(3):316-335.
10. van Vlerken L, Amiji M. Multi-functional polymeric nanoparticles for tumour-targeted drug delivery. Expert Opinion on Drug Delivery. 2006;3(2):205-216.
11. Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan M, Kumar R et al. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids and Surfaces B: Biointerfaces. 2003;28(4):313-318.
12. Okitsu K, Mizukoshi Y, Yamamoto T, Maeda Y, Nagata Y. Sonochemical synthesis of gold nanoparticles on chitosan. Materials Letters. 2007;61(16):3429-3431.
13. Tsuji M, Hashimoto M, Nishizawa Y, Tsuji T. Preparation of Gold Nanoplates by a Microwave-polyol Method. Chemistry Letters. 2003;32(12):1114-1115.
14. Kundu S, Maheshwari V, Niu S, Saraf R. Polyelectrolyte mediated scalable synthesis of highly stable silver nanocubes in less than a minute using microwave irradiation. Nanotechnology. 2008;19(6):065604.
15. Hahn H. Unique Features and Properties of Nanostructured Materials. Advanced Engineering Materials. 2003;5(5):277-284.
16. Shankar S, Rai A, Ankamwar B, Singh A, Ahmad A, Sastry M. Biological synthesis of triangular gold nanoprisms. Nature Materials. 2004;3(7):482-488.
17. Willner I, Baron R, Willner B. Growing Metal Nanoparticles by Enzymes. Advanced Materials. 2006;18(9):1109-1120.
18. Nair S, Sasidharan A, Divya Rani V, Menon D, Nair S, Manzoor K et al. Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells. Journal of Materials Science: Materials in Medicine. 2008;20(S1):235-241.
19. Njagi E, Huang H, Stafford L, Genuino H, Galindo H, Collins J et al. Biosynthesis of Iron and Silver Nanoparticles at Room Temperature Using Aqueous Sorghum Bran Extracts. Langmuir. 2011;27(1):264-271.
20. Li S, Shen Y, Xie A, Yu X, Qiu L, Zhang L et al. Green synthesis of silver nanoparticles using Capsicum annuum L. extract. Green Chemistry. 2007;9(8):852.
21. Rajamanickam V, Rajasekaran A, Quine DS, Jesupillai M, Sabitha R. Anthelmintic activity of the flower extract of Couroupita guianensis. The Internet Journal of Alternative Medicine. 2009;8(1).
22. Al-Dhabi N, Balachandran C, Raj M, Duraipandiyan V, Muthukumar C, Ignacimuthu S et al. Antimicrobial, antimycobacterial and antibiofilm properties of Couroupita guianensis Aubl. fruit extract. BMC Complementary and Alternative Medicine. 2012;12(1).
23. Pinheiro M, Fernandes S, Fingolo C, Boylan F, Fernandes P. Anti-inflammatory activity of ethanol extract and fractions from Couroupita guianensis Aublet leaves. Journal of Ethnopharmacology. 2013;146(1):324-330.
24. Elumalai A, Naresh V, Eswaraiah MC, Narendar P, Kumar R. Evaluation of antiulcer activity of Couroupita guianensis Aubl leaves. Asian Journal of Pharmacy and Technology. 2012; 2(2):64-66.
25. Uppala P, Krishna M, Kumar B, Ramji D. Evaluation of Anthelmintic Activity of the Chloroform and Aqueous Extracts of leaves of Couroupita guianensis on Pheretima posthuma by Worm Motility Assay Method. Research Journal of Pharmacology and Pharmacodynamics. 2016;8(3):118-122.
26. Rumzhum NN, Rahman M, Islam AFMR. CNS Depressant Effect of Crude Ethanolic Extract of Flowers of Couroupita guianensis Aubl. in Swiss-albino Mice. Research Journal of Pharmacy and Technology. 2012;5(5):615-618.
27. Mary JE, Inbathamizh L. Green synthesis and characterization of nano silver using leaf extract of Morinda pubescens. Asian Journal of Pharmaceutical and Clinical Research. 2012; 5(Suppl1):159-162.
28. Pawar P, Chaudhari S. Size controlled Bio-directed synthesis of Ag Metal Nanoparticles using Tulsi (Ocimum tenuiflorum) leaves extract. Asian Journal of Research in Chemistry. 2017;10(5):646-650.
29. Padmavathi Y, Anjali A, Babu NR, Kumar RP. Development and validation of new FTIR method for quantitative analysis of gliclazide in bulk and pharmaceutical dosage forms. Asian Journal of Research in Chemistry. 2017;10(3):377-382.
30. Selvakannan P, Mandal S, Phadtare S, Gole A, Pasricha R, Adyanthaya S et al. Water-dispersible tryptophan-protected gold nanoparticles prepared by the spontaneous reduction of aqueous chloroaurate ions by the amino acid. Journal of Colloid and Interface Science. 2004;269(1):97-102.
31. Raveendran P, Fu J, Wallen S. Completely “Green” Synthesis and Stabilization of Metal Nanoparticles. Journal of the American Chemical Society. 2003;125(46):13940-13941.
32. Willett R, Baldwin K, West K, Pfeiffer L. Differential adhesion of amino acids to inorganic surfaces. Proceedings of the National Academy of Sciences. 2005;102(22):7817-7822.
33. Haverkamp R, Marshall A. The mechanism of metal nanoparticle formation in plants: limits on accumulation. Journal of Nanoparticle Research. 2008;11(6):1453-1463.
34. Glusker JP, Katz AK, Bock CW. Metal Ions in Biological Systems. Rigaku Journal. 1999;16(2): 8‒16.
35. Wiley B, Im S, Li Z, McLellan J, Siekkinen A, Xia Y. Maneuvering the Surface Plasmon Resonance of Silver Nanostructures through Shape-Controlled Synthesis. The Journal of Physical Chemistry B. 2006;110(32):15666-15675.
36. Tyagi S, Alam T, Khan M, Tarannum H, Chauhan N. Biosynthesis of silver nanoparticles using medicinal plant Anacyclus pyrethrum and its antibacterial efficacy. Asian Journal of Research in Chemistry. 2018;11(3):515-520.
37. Zaheer Z, Rafiuddin. Silver nanoparticles to self-assembled films: Green synthesis and characterization. Colloids and Surfaces B: Biointerfaces. 2012;90:48-52.
38. Shankar S, Ahmad A, Pasricha R, Sastry M. Bioreduction of chloroaurate ions by geranium leaves and its endophytic fungus yields gold nanoparticles of different shapes. Journal of Materials Chemistry. 2003;13(7):1822.
39. Song J, Kim B. Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess and Biosystems Engineering. 2008;32(1):79-84.
40. Shankar S, Rai A, Ahmad A, Sastry M. Rapid synthesis of Au, Ag, and bimetallic Au core–Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. Journal of Colloid and Interface Science. 2004;275(2):496-502.
41. Sintubin L, Verstraete W, Boon N. Biologically produced nanosilver: Current state and future perspectives. Biotechnology and Bioengineering. 2012;109(10):2422-2436