Divya Yada, T. Sivakkumar, M. Sudhakar
Divya Yada1*, T. Sivakkumar2, M. Sudhakar1
1Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Maisammaguda, Dhulapally, Secunderabad - 14.TS.
2Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram TN.
Volume - 14,
Issue - 5,
Year - 2021
The current line of investigation was focused at perusing the presence of phytochemical constituents, investigation of total phenol and flavonoid content, the antioxidant potential of various extracts of Caralluma adscendens whole plant using various in-vitro assays. The dried plant powder was extracted with various solvents based on polarity (Pet ether, Chloroform, Ethyl acetate, Ethanol and Aqueous) by hot continuous extraction in Soxhlet's apparatus and Extracts were dried. Phytoconstituents present in each extract was examined by performing preliminary phytochemical screening. Total Phenolic Content (TPC), Total Flavonoid Content (TFC) and Antioxidant potential for crude extracts were studied by DPPH, nitric oxide scavenging and FRAP methods. The total phenolic content and flavonoid content of Ethanolic extract of plant was found to be 80.08±0.629mg and 70.88±1.170mg of GAE and Quercetin equivalents respectively. The Ethanolic extract exhibited potent antioxidant activity as determined by 2,2-diphenyl-1-picrylhydrazyl(DPPH), nitric oxide scavenging and ferric reducing antioxidant power assays(FRAP) than the other extracts. The IC50 values for the Ethanolic extract of Caralluma adscendens was found to be 214.765±0.224 µg/ml and 215.928±0.506µg/ml by DPPH and nitric oxide scavenging assays respectively.
Cite this article:
Divya Yada, T. Sivakkumar, M. Sudhakar. Phytochemical Evaluation and In-vitro Antioxidant Potential of whole plant of Caralluma adscendens. Research Journal of Pharmacy and Technology. 2021; 14(5):2774-8. doi: 10.52711/0974-360X.2021.00489
Divya Yada, T. Sivakkumar, M. Sudhakar. Phytochemical Evaluation and In-vitro Antioxidant Potential of whole plant of Caralluma adscendens. Research Journal of Pharmacy and Technology. 2021; 14(5):2774-8. doi: 10.52711/0974-360X.2021.00489 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-5-73
1. Valko M, Leibfritz D, Moncol J, Cronin M, and Mazur M, et al. Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology. 2007; 39: 44-84.
2. Sies H. Oxidative stress: Oxidants and antioxidants. Experimental Physiology. 1997; 82:291-295.
3. Hybertson BM, Gao B, Bose SK, and McCord JM. Oxidative stress in health and disease: the therapeutic potential ofNrf2 activation. Molecular Aspects of Medicine. 2011; 32 (4): 234–246.
4. Liang D, Zhou Q, Gong W, Wang Y, Nie Z, He H, et al. Studies on the antioxidant and hepatoprotective activities of polysaccharides from Talinum triangulare. Journal of Ethnopharmacology. 2011; 136(2): 316–321.
5. Rains JL and Jain SK. Oxidative stress, insulin signaling, and diabetes. Free Radical Biology and Medicine. 2011; 50(5): 567–575.
6. Masella R, Di Benedetto R, Vari R, Filesi C and Giovannini C. Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione related enzymes. Journal of Nutritional Biochemistry. 2005; 16(10): 577–586.
7. Dai J and Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010;15(10):7313–7352.
8. Yingming P, Ying L, Hengshan W, Min L. Antioxidant activities of several Chinese medicine herbs. Journal of Food and Chemistry. 2004; 88(3): 347–350.
9. Zakaria MNM, Islam MW, Radhakrishnan R, Chen HB, Kamil M, Al-Gifri AN et al. Anti-nociceptive and anti-inflammatory properties of Caralluma Arabica. Journal of Ethnopharmacology .2001; 76:155-158.
10. Kamalakannan S, Rajendran R, Venkatesh R, Clayton P, Akbarsha AK. Anti-obesegenic and Anti-Atherosclerotic Properties of Caralluma fimbriata Extract. Journal of Nutritional Metabolism. 2011; Article ID 285301: 6.
11. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Foline-Ciocalteu reagent. Methods of Enzymology. 1999; 299:152-78.
12. Chang C, Yang M, Wen H, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food Drug Analysis. 2002; 10:178-82.
13. Ilahi I, Samar S, Khan I, Ahmad I. In vitro antioxidant activities of four medicinal plants on the basis of DPPH free radical scavenging. Pakistan journal of Pharmaceutical sciences. 2013; 26:949-952.
14. Garrat DC. The Quantitative Analysis of drugs.Chapman and Hall, Japan.1964; 3: 456 - 458.
15. Oyaizu M. Studies on products of browning reactions: Antioxidant activity of products of browning reaction prepared from glucosamine. Japan Journal of Nutrition 1986; 44: 307-315.
16. Bors W, Heller W, Michel C, Saran M. Flavonoids as antioxidant: Determination of radical scavenging efficiencies. Methods in Ezymology. 1990; 186:343-355.
17. Lata H, Ahuja GK. Role of free radicals in health and disease. Indian Journal of Physiology and Allied Sciences. 2003; 57:124‐28.
18. Ialenti, Moncada S, Di Rosa M. Modulation of adjuvant arthritis by endogenous nitric oxide. British Journal of Pharmacology. 1993;110: 701‐705.