Lavanya D, Pooja Rao, Soundarya S, Surabhi T.S, Prashith Kekuda T.R
Lavanya D, Pooja Rao, Soundarya S, Surabhi T.S, Prashith Kekuda T.R*
Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, Balraj Urs Road, Shivamogga - 577201, Karnataka, India.
Volume - 14,
Issue - 5,
Year - 2021
Objectives: Alangium salviifolium L. is one of the medicinally important plant belonging to the family Cornaceae. The present study evaluates antibacterial, antifungal and antioxidant activity of leaf and fruit extracts of A. salviifolium. Methods: Extraction of shade dried and powdered leaf and fruit was carried out by maceration technique. Antibacterial and antifungal activity was evaluated by agar well diffusion method and poisoned food technique, respectively. Antioxidant activity was determined by antiradical (DPPH and ABTS assays) and ferric reducing assays. Results: Both leaf and fruit extracts displayed inhibitory activity against test bacteria. Fruit extract was shown to be more effective against test bacteria when compared to leaf extract. Leaf extract was more effective in causing inhibition of mycelial growth of test fungi when compared to fruit extract. Both leaf and fruit extracts scavenged DPPH and ABTS radicals dose dependently and exhibited ferric reducing activity. Conclusions: The results of the present study indicated the potential of leaf as well as fruit of A. salviifolium to exhibit antimicrobial and antioxidant activities which justifies the traditional medicinal uses of the plant.
Cite this article:
Lavanya D, Pooja Rao, Soundarya S, Surabhi T.S, Prashith Kekuda T.R. Antibacterial, Antifungal and Antioxidant activity of leaf and fruit of Alangium salviifolium L. (Cornaceae). Research Journal of Pharmacy and Technology. 2021; 14(5):2745-9. doi: 10.52711/0974-360X.2021.00484
Lavanya D, Pooja Rao, Soundarya S, Surabhi T.S, Prashith Kekuda T.R. Antibacterial, Antifungal and Antioxidant activity of leaf and fruit of Alangium salviifolium L. (Cornaceae). Research Journal of Pharmacy and Technology. 2021; 14(5):2745-9. doi: 10.52711/0974-360X.2021.00484 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-5-68
1. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999; 12(4): 564-582.
2. Sharma A, Verma R, Ramteke P. Antibacterial activity of some medicinal plants used by tribals against Uti causing pathogens. World Appl Sci J. 2009; 7(3): 332-339.
3. Khan R, Islam B, Akram M, Shakil S, Ahmad A, Ali SM, Siddiqui M, Khan AU. Antimicrobial activity of five herbal extracts against multi drug resistant (MDR) strains of bacteria and fungus of clinical origin. Molecules. 2009; 14: 586-597.
4. Baskaran R, Mohan PM. In vitro antibacterial activity of leaf extracts of Rhizophora mucronata L. against multi drug resistant Vibrio spp. Isolated from marine water Lobster’s larvae hatcheries. Indian J Geo-Marine Sci. 2012; 41(3): 218-222.
5. Wikaningtyas P, Sukandar EY. The antibacterial activity of selected plants towards resistant bacteria isolated from clinical specimens. Asian Pac J Trop Biomed. 2016; 6(1): 16–19.
6. Gupta PD, Birdi TJ. Development of botanicals to combat antibiotic resistance. J Ayurveda Integr Med. 2017; 8: 266-275.
7. Raghavendra HL, Kekuda PTR, Akarsh S, Ranjitha MC, Ashwini HS. Phytochemical analysis, antimicrobial and antioxidant activities of different parts of Pleocaulus sessilis (Nees) Bremek (Acanthaceae). Int J Green Pharm. 2017; 11(2): 98-107.
8. Makovitzki A, Viterbo A, Brotman Y, Chet I, Shai Y. Inhibition of fungal and bacterial plant pathogens in vitro and in planta with ultrashort cationic lipopeptides. Appl Environ Microbiol. 2007; 73(20): 6629-6636.
9. Tapwal A, Nisha, Garg S, Gautam N, Kumar R. In vitro antifungal potency of plant extracts against five phytopathogens. Braz Arch Biol Technol. 2011; 54(6): 1093-1098.
10. Kekuda PTR, Akarsh S, Nawaz NAS, Ranjitha MC, Darshini SM, Vidya P. In vitro antifungal activity of some plants against Bipolaris sorokiniana (Sacc.) Shoem. Int J Curr Microbiol Appl Sci. 2016; 5(6): 331-337.
11. Shuping DSS, Eloff JN. The use of plants to protect plants and food against fungal pathogens: A review. Afr J Tradit Complement Altern Med. 2017; 14(4): 120-127.
12. Marei GIK, Abdelgaleil SAM. Antifungal potential and biochemical effects of Monoterpenes and Phenylpropenes on plant pathogenic fungi. Plant Protect Sci. 2018; 54(1): 9–16.
13. Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol. 2001; 54: 176-186.
14. Lu J, Lin PH, Yao Q, Chen C. Chemical and molecular mechanisms of antioxidants: experimental approaches and model systems. J Cell Mol Med. 2010; 14(4): 840-860.
15. Pisoschi AM, Negulescu GP Methods for total antioxidant activity determination: A review. Biochem Anal Biochem. 2011; 1(1): 106.
16. Junaid S, Rakesh KN, Dileep N, Poornima G, Kekuda PTR, Mukunda S. Total phenolic content and antioxidant activity of seed extract of Lagerstroemia speciosa L. Chem Sci Trans. 2013; 2(1): 75-80.
17. Alam Md, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J. 2013; 21: 143-152.
18. Anbudhasan P, Surendraraj A, Karkuzhali S, Sathishkumaran S. Natural antioxidants and its benefits. International Journal of Food and Nutritional Sciences. 2014; 3(6): 225-232.
19. Bhat GK. Flora of South Kanara. Akriti Prints, Mangalore, India, 2014.
20. Survase SA, Raut SD. Ethnobotanical Study of some tree medicinal plants in Marathwada, Maharashtra. Journal of Ecobiotechnology 2011; 3(2): 17-21.
21. Pradheeps M, Poyyamoli G. Ethnobotany and utilization of plant resources in Irula villages (Sigur plateau, Nilgiri Biosphere Reserve, India). J Med Plants Res. 2013; 7(6): 267-276.
22. Raju RY, Yugandhar P, Savithramma N. Documentation of ethnomedicinal knowledge of hilly tract areas of East Godavri District of Andhra Pradesh, India. Int J Pharm Pharm Sci. 2014; 6(4): 369-374.
23. Patel NB, Patel KB. Ethnomedicinal plants used by the tribals of district Sabarkantha, Gujarat, India. J Med Plants Stud. 2015; 4(3): 179-181.
24. Rao SS. Ethno botanical study of medicinal plants of Sri Pancha Narasimha Swamy and Sri Matsyagiri Narasimha Swamy. J Med Plants Stud. 2015; 3(3): 37-42.
25. Kachhi RS, Saket VK, Sharma P, Singh P. Treatment of tuberculosis using ethno-medicinal plants of Amarkantak region. Asian J Anim Vet Adv. 2018; 13(1): 52-60.
26. Jain VC, Patel NM, Shah DP, Patel KP, Joshi BH. Antioxidant and antimicrobial activities of Alangium salviifolium (leaf) Wang root. Global J Pharmacol. 2010; 4(1): 13-18.
27. Pavunraj M, Pulraj GM, Kumar SS, Rao MRK, Ignacimuthu S. Feeding deterrence, larvicidal and haemolymph protein profiles of an Indian traditional medicinal plant Alangium salviifolium (L.F.) Wangerin on cluster caterpillar, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Arch Phytopathol Plant Protect. 2012; 45(17): 2066-2075.
28. Prakash UNK, Bhuvaneswari S, Preethy S, Rajalakshmi N, Saranya M, Anto JR, Arokiyaraj S. Studies on antimicrobial, antioxidant, larvicidal, pesticidal activity and phytochemistry of leaves of Alangium salviifolium (L.f) Wang. Int J Pharm Pharm Sci. 2013; 5(2): 86-89.
29. Shravya S, Vinod BN, Sunil C. Pharmacological and phytochemical studies of Alangium salviifolium Wang. – A review. Bull Fac Pharm Cairo Univ. 2017; 55(2): 217-222.
30. Patel AK, Manigauha A. Antioxidant and antidiabetic activity of isolated flavonoids from Alangium salviifolium leaves extracts. Int J Green Pharm. 2018; 12(2): 82-90.
31. Sharanappa R, Vidyasagar GM. Preliminary screening of ethnomedicinal plants for antibacterial activity. Int J Pharm Sci Res. 2015; 6(9): 3928-3935.
32. Dahiya P, Purkayastha S. Phytochemical screening and antimicrobial potentials of Alangium salviifolium and Piper longum against multi-drug resistant bacteria from clinical isolates. Int J Pharm Pharm Sci 2011; 3(S5): 462-465.
33. Sree SD, Sreeramulu A. Inhibitory effect of plant extracts on the management of bacterial leaf spot pathogen (Xanthomonas axonopodis pv. vesicatoria) infecting Capsicum frutescens L. Journal of Natural Remedies 2002; 2(2): 191-194.
34. Pandian M R, Banu G S, Kumar G. A study of the antimicrobial activity of Alangium salviifolium. Indian J Pharmacol. 2006; 38: 203-204.
35. Wuthi-udomlert M, Prathanturarug S, Wongkrajang Y. Antifungal activity and local toxicity study of Alangium salviifolium subsp hexapetalum. Southeast Asian J Trop Med Public Health 2002; 33(S3): 152-154.
36. Prathyusha P, Subramanian M. S. Antifungal activity of seeds of Alangium salviifolium (L.F.) Wangerin (Alangiaceae). Biosci Biotech Res Asia 2011; 8(2): 865-868.
37. Chung Y, Chien C, Teng K, Chou S. Antioxidative and mutagenic properties of Zanthoxylum ailanthoides Sieb & Zucc. Food Chemistry 2006; 97: 418-425.
38. Rajurkar NS, Hande SM. Estimation of phytochemical content and antioxidant activity of some selected traditional Indian medicinal plants. Indian J Pharm Sci. 2011; 73(2): 146-151.
39. Sowndhararajan K, Kang SC. Free radical scavenging activity from different extracts of leaves of Bauhinia vahlii Wight & Arn. Saudi J Biol Sci. 2013; 20(4): 319–325.
40. Basu P, Maier C. In vitro antioxidant activities and polyphenol contents of seven commercially available fruits. Pharmacognosy Res. 2016; 8(4): 258-264.
41. Zahan R, Nahar L, Haque M, Nesa ML, Alam Z. Antioxidant and antidiabetic activities of Alangium salviifolium and Bombax ceiba. Dhaka Univ J Pharm Sci. 2013; 12(2): 159-163.
42. Nahar L, Zahan R, Mosaddik A, Islam S, Haque A, Fazal A, Jesmin M. Antioxidant and antitumor activity of chloroform extract of Alangium salviifolium flowers. Phytopharmacology. 2012; 2(1): 123-134.
43. Sakthidevi G, Mohan V R, Jeeva S. In vitro antioxidant activity of Alangium salviifolium (L.f.) Wang (Alangiace) stem. International Journal of Advances in Pharmacy Biology and Chemistry. 2014: 3(3): 589-596.
44. Vats S. Evaluation of antioxidant potential of Alangium salviifolium (L.f.) Wangerin. Researcher. 2015; 7(2): 92-94.
45. Sakthidevi G, Mohan VR, Jeeva S. In vitro antioxidant activity leaf extracts of Alangium salviifolium (L.f.) Wang (Alangiaceae). Bioscience Discovery. 2014; 5(1):74-81