R. Balabhaskar, K. Vijayalakshmi
R. Balabhaskar1*, K. Vijayalakshmi2
1Research Scholar, Department of Biochemistry, Bharathiar University, Coimbatore - 641046.
2Associate Professor, Department of Biochemistry, Bharathi Womens’ College, Chennai - 600 108.
Volume - 14,
Issue - 5,
Year - 2021
Gas Chromatography-Mass Spectrometry (GC-MS) is normally used for direct analysis of chemical components existing in herbal medicines. The medicinal plants are having numerous bioactive components which are identified even at less than 1ng by using GC-MS or LC-MS analysis. The aim of this study is to identify the secondary metabolites present in the leaves of B. tomentosa using gas chromatography-mass spectrometry (GC-MS) analysis. In the present study the ethanol extract of the leaves of Bauhinia tomentosa has been subjected to GC-MS analysis, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis revealed the presence of 14 secondary metabolites. These compounds were identified by comparing their retention times and peak areas with those from the literature and by interpretation of the mass spectra. The major secondary metabolites were DL-.alpha.-tocopherol (14.84%), 2-[(trimethylsilyl oxy]-, methyl ester, 1-alpha,2-alpha.-epoxy-1-beta-methylcholesta-4,6-dien-3-one (12.93%), pentacosenoic acid (12.71%), phytol (10.28%), Ethyl Isoallocholate (8.197%), Spirost-8-en-11-one-3-hydroxy-,(3-beta,5 alpha,14 beta,20 beta, 22 beta,25R)-(8.162%), Urs-12-en-28-ol (6.675%), 1-Octadecyne (5.702%) and Cholest-8-en-3-beta-ol,Acetate (5.426%). The compounds having area less that 5% were considered of no significance. These findings suggest that the presence of these secondary metabolites may be the cause for the properties exhibited by Bauhinia tomentosa. Thus, presence of various bioactive compounds justifies the use of the leaf for various ailments by traditional practitioners.
Cite this article:
R. Balabhaskar, K. Vijayalakshmi. Identification of Secondary Metabolites from the Ethanol extract of the leaves of Bauhinia tomentosa by GC-MS Analysis. Research Journal of Pharmacy and Technology. 2021; 14(5):2735-1. doi: 10.52711/0974-360X.2021.00482
R. Balabhaskar, K. Vijayalakshmi. Identification of Secondary Metabolites from the Ethanol extract of the leaves of Bauhinia tomentosa by GC-MS Analysis. Research Journal of Pharmacy and Technology. 2021; 14(5):2735-1. doi: 10.52711/0974-360X.2021.00482 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-5-66
1. Manian R, Anusuya N, Siddhuraju P, Manian S. The antioxidant activity and free radical scavenging potential of two different solvent extracts of Camellia sinensis (L.) O. Kuntz, Ficus bengalensis L. and Ficusracemosa L. Food Chem. 2008; 107(3): 1000-1007.
2. Nosheen Akhtar, Ihsan-ul-Haq, Bushra Mirza. Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species. Arabian Journal of Chemistry. 2018; 11 (8): 1223-1235.
3. Quiroga EN, Sampietro AR, Vattuone MA. Screening antifungal activities of selected medicinal plants. J. Ethnopharmacol. 2001; 74: 89-96.
4. Shiv Kumar Gupta. Phytopharmacognostic Investigation of Bauhinia tomentosa Linn J.Adv.Sci.Res.,2011; 2(2): 01-04.
5. Ragavan, B, Krishnakumari, S. Antidiabetic effect of T. Arjuna bark extract in alloxan induced diabetic rats. Indian J. of Clinical Biochemistry, 2006; 21: 123-128.
6. Suja Pandian R, Thajun Noora A. GC-MS Analysis of Phytochemical Compounds Present in the Leaves of Citrus medica. L. Research J. Pharm. and Tech. 2019; 12(4): 1823-1826.
7. Sathammai Priya, Ambikapathy, Panneerselvam, Sangeetha. Gas chromatography and mass spectroscopy analysis of phytoactive components on the seed extract of Caesalpinia bonducella. Research J. Pharm. and Tech. 2019; 12(10): 4628-4634.
8. Merlin NJ, Parthasarathy V, Manavalan R, Kumaravel S. Chemical Investigation of Aerial Parts of Gmelina asiatica Linn by GC-MS. Pharmacog Res. 2009; 1(3): 152-156.
9. Sahaya SS, Janakiraman N, Johnson M. Phytochemical analysis of Vitex altissima L. using UV-Vis, FTIR and GC-MS. Int J Pharm Sci Drug Res. 2012; 4(1): 56-62.
10. Mythili K, Umamaheswara Reddy C, Chamundeeswari D and Manna PK. GC-MS analysis of phytocomponents and in vitro inhibitory effects of Calanthe triplicata. Journal of Natural Products. 2013; 6: 141-146.
11. Gopalakrishnan S, Vadivel E. GC-MS Analysis of some bioactive constituents of Mussaenda frondosa Linn. Int J Pharma and Bio Sci. 2011; 2(1): 313-320.
12. Senthil Kumar V, Sathish Kumar G, Siva Ramakrishnan S, Sujatha K, Razia M. Evaluation of phytoconstituents, In vitro antioxidant and antimicrobial activities of Agaricus bisporus. Int J Pharmacy Pharmaceu Sci. 2016; 8(3), 67-71.
13. Paulpriya K, Tresina PS and Mohan VR. Assessment of Bioactive Constituents by GC-MS of Crotalaria longipes Wight & Arn.: An Endemic Plant. International Journal of Pharmacognosy and Phytochemical Research. 2014; 6(4):1043-1048.
14. Nagala S and Tamanam RR. Artocarpus methanol extract seed oils-a comparative study. International Journal of Pharmaceutical Sciences and Research. 2017; 8(4):1781.
15. Thomas E, Aneesh TP, Thomas DG, Anandan R. GC-MS Analysis of phytochemical compounds present in the rhizomes of Nervilia aragoana gaud. Asian J Pharm Clin Res. 2013; 6(3):68-74.
16. Kallio H, Yang B, Peippo P, Tahvonen R and Pan R. Triacylglycerols, Glycerophospholipids, Tocopherols, and Tocotrienols in Berries and Seeds of Two Subspecies (ssp. sinensis and mongolica) of Sea Buckthorn (Hippopha> ë rhamnoides). Journal of Agricultural and Food Chemistry. 2002; 50(10): 3004-3009.
17. Ali SS, Kasoju N, Luthra A and Singh A, Sharanabasava H, Sahu A, Bora U. Indian medicinal herbs as sources of antioxidants. Food Research International. 2008; 41(1):1-15.
18. Kaiser S, DiMascio P, Murphy ME and Sies H. Physical and chemical scavenging of singlet molecular oxygen by tocopherols. Arch Biochem Biophys.1990; 277(1): 101-108.
19. Cheesman KH and Slatter TF. An introduction to free radical biochemistry. Brit Med Bull. 1993; 49(3): 481- 493.
20. Likoff RO, Guptill DR, Lawrence LM, McKay CC, Mathias MM, Nockels CF and Tengerdy RP. Vitamin E and aspirin depress prostaglandins in protection of chickens against Escherichia coli infection. Am J Clin Nutr.1981; 34(2):245-251.
21. Guimarães AG, Oliveira GF, Melo MS, Cavalcanti SC, Antoniolli AR, Bonjardim LR, Silva FA, Santos JP, Rocha RF, Moreira JC, Araújo AA, Gelain DP, Quintans-Júnior LJ. Bioassay-guided evaluation of antioxidant and antinociceptive activities of carvacrol. Basic and Clinical Pharmacology and Toxicology, 2010; 107 (6): 949–957
22. Lima RK and Cardoso MG. Família Lamiaceae: importantes óleos essenciais com ação biológica e antioxidante, Fitos, 2007; 3:14–24.
23. Serafini MR, Santos RC, Guimarães AG, Dos Santos JP, da Conceicão Santos AD, Alves IA, Gelain DP, de Lima Nogueira PC, Quintans-Júnior LJ, Bonjardim LR, de Souza Araújo AA. Morinda citrifolia linn leaf extract possesses antioxidant activities and reduces nociceptive behavior and leukocyte migration, Journal of Medicinal Food, 2011; 14 (10): 1159–1166.
24. Hoelzl C, Bichler J, Ferk F et al., Methods for the detection of antioxidants which prevent age related diseases: a critical review with particular emphasis on human intervention studies. Journal of Physiology and Pharmacology, 2005; 56 (2): 49-64.
25. Halliwell B. Antioxidant characterization. Methodology and mechanism, Biochemical Pharmacology, 1995; 49(10): 1341–1348.
26. Huang D, Boxin OU, and Prior RL. The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry, 2005; 53 (6): 1841–1856.
27. Shukla S, Mehta A, Bajpai VK, and Shukla S. In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert. Food and Chemical Toxicology, 2009; 47(9): 2338–2343.
28. Daffodil E, Uthayakumari F and Mohan V. GC-MS determination of bioactive compounds of Curculigo orchioides gaertn. Science Research Reporter 2012; 2(3): 198-201.
29. Sarada K, Margret RJ, Mohan V. GC–MS Determination of Bioactive Components of Naringi crenulata (Roxb) Nicolson. Int J Chem Tech Research 2011; 3(3): 1548-1555.
30. Griffiths MJD, Messent M and Macallister RJ and Evans TW. Aminoguanidine selectively inhibits inducible nitric oxide synthase. Br. J. Pharmacol., 1993; 110(3): 963 – 968.
31. Laszlo F, Evans SM and Whittle BJR. Aminoguanidine inhibits both constitutive and inducible nitric oxide synthase isoforms in rat intestinal microvasculature in vivo. Eur. J. Pharmacol., 1995; 272: 169 – 175.
32. Sorrentino R, Sautebin L and Pinto A. Effect of methylguanidine, guanidine and structurally related compounds on constitutive and inducible nitric oxide synthase activity. Life Sci., 1997; 61: 1283 – 1291.
33. Kujumgiev A, Bankova V, Ignatova A and Popov S Antibacterial activity of propolis, some of its components and their analogs. Pharmazie.1993; 48(10): 785 – 786.
34. Anand Gideon V.GC-MS analysis of phytochemical components of Pseudoglochidion anamalayanum Gamble: An endangered medicinal tree. Asian Journal of Plant Science and Research, 2015; 5(12): 36-41.