Author(s): Karthikeyan Sekar, Rajeswary Hari, P. Ramya, N. Pusphavalli, R. Savitha

Email(s): rajihar@gmail.com

DOI: 10.52711/0974-360X.2021.00455   

Address: Karthikeyan Sekar, Rajeswary Hari*, P. Ramya, N. Pusphavalli, R. Savitha
Department of Biotechnology, Dr. M.G.R. Educational and Research Institute, Deemed to be University, Chennai - 95 Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 5,     Year - 2021


ABSTRACT:
In the present investigation an attempt was made to evaluate the in vitro and in silico anti-gout arthritic activity of ethanolic (EECF) and aqueous extracts (AECF) of leaves of Cadaba fruticosa. The in vitro anti-gout arthritic activity of EECF and AECF was evaluated in terms of their inhibitory potential of xanthine oxidase, proteinase enzymes as well as protein denaturation and membrane stabilization using standard protocols. For the analysis of in silico anti-gout arthritic activity, molecular docking was performed for the GC–Ms derived 15 phyto constituents using patch dock server to find a suitable antagonistic ligand for the enzymes cyclooxygenase I and matrix metalloproteinase IV since they are the key enzymes responsible for pain and degenerative changes. Among the EECF and AECF extracts the EECF extract exhibited higher inhibitory activity of the xanthine oxidase and proteinase enzyme. At the concentrations of 800 and 1000µg/ml the observed inhibitory activity was almost similar to the positive drug Allopurinol and Acetyl salicylic acid. Based on the docking score and activation energy the two phyto constituents Quercetin and Cadabicinediacetate inhibited the enzymes cyclooxygenase I and matrix metalloproteinase IV and serves as a better antagonistic ligand to suppress the pain and joint destruction. It may be concluded that the leaves of Cadaba fruticosa may further developed into a effective drug for the management of gouty arthritis due to its multi targeted inhibitory activity of several inflammatory mediators.


Cite this article:
Karthikeyan Sekar, Rajeswary Hari, P. Ramya, N. Pusphavalli, R. Savitha. Anti-gout arthritic activities of Ethanolic and Aqueous leaf extracts of Cadaba fruticosa- An In vitro and In silico studies. Research Journal of Pharmacy and Technology. 2021; 14(5):2587-2. doi: 10.52711/0974-360X.2021.00455

Cite(Electronic):
Karthikeyan Sekar, Rajeswary Hari, P. Ramya, N. Pusphavalli, R. Savitha. Anti-gout arthritic activities of Ethanolic and Aqueous leaf extracts of Cadaba fruticosa- An In vitro and In silico studies. Research Journal of Pharmacy and Technology. 2021; 14(5):2587-2. doi: 10.52711/0974-360X.2021.00455   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-5-39


REFERENCE:
1.    Sherwood ER, Toliver-Kinsky T. Mechanisms of the inflammatory response. Best Practice and Research Clinical Anaesthesiology. 2004; 18(3): 385-405.
2.    Nathan C, Ding A. Nonresolving Inflammation. Cell. 2010; 140(6): 871-882.
3.    Bieber JD, Terkeltaub RA. Gout: on the brink of novel therapeutic options for an ancient disease. Arthritis & Rheumatism.2004; 50(8): 2400–2414.
4.    Choi HK, Atkinson K, Karlson EW, Willett W, Curhan G. Purine-rich foods, dairy and protein intake, and the risk of gout in men. New England Journal of Medicine.2004; 350(11): 1093–1103.
5.    Akahoshi T, Murakami Y, Kitasato H. Recent advances in crystal-induced acute inflammation. Current Opinion inRheumatology. 2007; 19(2): 146–150.
6.    Terkeltaub R. Pathogenesis and treatment of crystal-induced inflammation in Arthritis and Allied Conditions. Lippincott Williams and Wilkins, Philadelphia, Pa, USA WJ. Koopmanand LW. Moreland Eds; 20042357–2372.
7.     Sabina EP, Rasool M, Mathew L, EzilRani P, Indu H.6-Shogaol inhibits monosodium urate crystal-induced inflammation-an in vivo and in vitro study. Food and Chemical Toxicology. 2010; 48(1): 229–235.
8.    Robinson PC. Gout – an update of aetiology, genetics, co-morbidities and management. An international Journal of Midlife Health and Beyond. 2018; 118(1): 67-73.
9.    Abhishek A, Roddy E, Doherty M. Gout – a guide for the general and acute physicians. Clinical Medicine 2017; 17(1): 54–59.
10.    Terkeltaub R. Gout. Novel therapies for treatment of gout and hyperuricemia. Arthritis Res Therapy. 2009;11(4): 236.
11.    Arokiyaraj S, Radha R, Martin S, Perinbam K.Phytochemical analysis and anti-diabetic activity of Cadabafruticosa. Indian Journal of Science and Technology. 2008; 1(6): 1-4.
12.    Yoganarasimhan SN. Medicinal Plants of India. Bangalore: Interline Publishing; 1996.p. 8.
13.    Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants (Raw Materials), New Delhi: CSIR Publications; 1979.p.43.
14.    Owen P, JohnsT. Xanthine oxidase inhibitory activity of north eastern North American plant remedies used for gout. Journal of Ethnopharmacology. 1999; 64(2): 149-160
15.    Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins especially with some biologically active proteins. Journal of Pharmacy and Pharmacology. 1968; 20(3): 169-173.
16.    Sakat SS, Archana RJ, Manoj NG. In¬vitro antioxidant and anti-inflammatory activity of methanol extract of oxalis corniculatalinn. international Journal of Pharmacy and Pharmaceutical Sciences. 2010; 2(1); 146-155.
17.    Oyedapo OO, Famurewa AJ. Anti-protease and membrane stabilizing activities of extracts of Fagrazanthoxiloides, Olaxsubscorpioides and Tetrapleuratetraptera. International Journal of Pharmacognosy.1995; 33(1): 65-69.
18.    Amudha M, Rani S. Assessing the bioactive constituents of Cadabafruticosa (L.) Druce through GC-MS. International Journal of Pharmaceutics and Pharmaceutical Science. 2014;6(2): 383-385.
19.    Telrandhe UB, Uplanchiwar V. Phyto-pharmacological perspective of Cadaba farinosa forsk. American Journal of Phytomedical Clinical Therapy. 2013;1(1): 11-22.
20.    Umapathy E, Ndebia EJ, Meeme A, Adam B, Menziwa P, Nkeh-Chungag BN, Iputo JE. An experimental evaluation of Albucasetosa aqueous extract on membrane stabilization, protein denaturation and white blood cell migration during acute inflammation, Journal of Medicinal Plants Research. 2010; 4(9): 789-795.
21.    Meyer MC, Rastogi P, Beckett CS, Mc Howat J. Phospholipase A2 Inhibitors as Potential Anti-Inflammatory Agents. Current Pharmaceutical Design.2005; 11(10): 1301-1312.
22.    Yousif G, Iskander GM and Eisa EB. Alkaloids of cadaba farinose and cadabarotundifolia. Fitoterapia.  1984: 55(1): 117-118.
23.    Koeberle A, Werz O. Multi-target approach for natural products in inflammation. Drug Discovery Today. 2014;19(12): 1871-1882

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags