Author(s): Evaluation of Hepatoprotective activity of Ethanolic Extract of Garuga pinnata Roxburgh leaves against Carbon tetrachloride induced Hepatotoxicity in rats.

Email(s): sdchavan.tkcp@gmail.com

DOI: 10.52711/0974-360X.2021.00419   

Address: Sandeep Chavan1*, Remeth Dias2, Chandrakant Magdum3
1Assistant Professor, Department of Pharmacology, Tatyasaheb Kore College of Pharmacy, Warananagar, Tal- Panhala, Dist- Kolhapur 416113 Maharashtra, India.
2Head of Pharmacy Department, Government Polytechnic Jalgaon, National Highway No. 6, Jalgaon, Pin- 425001 Maharashtra, India.
3Professor and Principal, Rajarambapu College of Pharmacy, Kasegaon, Sangli, Pin- 415404 Maharashtra, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 5,     Year - 2021


ABSTRACT:
In this study we investigated the in vivo Hepatoprotective activity of ethanolic extract of Garuga pinnata (EEGP) leaves in Carbon tetrachloride (CCl4) induced hepatotoxicity using wistar rats of either sex as model. Hepatotoxicity was induced by the administration of CCl4 intraperitoneally (0.125ml CCl4 in liquid paraffin (1:1) per 100g body weight). Garuga pinnata leaves extract at different dose levels (200 and 400mg/kg, p.o.) showed the dose dependant hepatoprotective effect and was compared with well known standard hepatoprotective Silymarain (100mg/kg). When groups were treated with CCl4, significant increase in serum biochemical parameters such as Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Glutamate Pyruvate Transaminase (SGPT), Alkaline phosphate (ALP), Acid Phosphate (ACP), Creatinine and alteration of tissue biochemical parameters such as reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (LPO), and the total proteins were observed. The histopathological examination of the CCl4 treated groups showed sinusoidal congestion, centrilobular necrosis, marked vacuolations and congestion. However, pretreatment with extract of leaves of Garuga pinnata significantly reduced the increased serum levels of biochemical parameters and restored antioxidant defense enzymes level to its normal. Moreover, histopathology of leaves extract treated groups showed normal architecture with minimal sinusoidal congestion. Taken together, our study concludes that EEGP to be a more potential agent for caring liver from CCl4 induced damage.


Cite this article:
Sandeep Chavan, Remeth Dias, Chandrakant Magdum. Evaluation of Hepatoprotective activity of Ethanolic Extract of Garuga pinnata Roxburgh leaves against Carbon tetrachloride induced Hepatotoxicity in rats. Research Journal of Pharmacy and Technology. 2021; 14(5):2375-0. doi: 10.52711/0974-360X.2021.00419

Cite(Electronic):
Sandeep Chavan, Remeth Dias, Chandrakant Magdum. Evaluation of Hepatoprotective activity of Ethanolic Extract of Garuga pinnata Roxburgh leaves against Carbon tetrachloride induced Hepatotoxicity in rats. Research Journal of Pharmacy and Technology. 2021; 14(5):2375-0. doi: 10.52711/0974-360X.2021.00419   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-5-3


REFERENCE:
1.    Guyton AC and Hall JE. Textbook of Medical Physiology. Elsevier Saunders. 2006; 11th Edn: 859-860.
2.    Sumeet KA et al. Burden of liver diseases in the world. Journal of Hepatology. 2019; 70: 151-171.
3.    Jadhav AG et al.  Hepatoprotective Activity of Oxalis corniculata Leaves against Carbon Tetrachloride Induced Hepatotoxicity in Female Albino Wistar Rats. Research Journal of Pharmacognosy and Phytochemistry. 2010; 2(2): 136-139.
4.    Sathish R et al.  Hepatoprotective and Anti Pyretic Activities of Methanolic Extract of Butea monosperma Lam Stem Bark in Wister Rats. Asian Journal of Pharmaceutical Research. 2011; 1(4): 130-133.
5.    Sonia Zaman. In vitro evaluation of antimicrobial, antioxidant and cytotoxic activities of Garuga pinnata leaves. Indo American Journal of Pharmaceutical Sciences. 2016; 3(3): 294-299.
6.    B. Lavanya and S. Thangamalathi. Garuga pinnata Roxburgh –An Update. International Journal of Institutional Pharmacy and Life Sciences. 2016; 6(2): 6-11.
7.    The Organisation for Economic Co-operation and Development. Guidelines on acute Oral toxicity. Revised Document. October 2005.
8.    Suneetha B et al. Hepatoprotective and Antioxidant Activity of Methanolic Extract of Actinodaphne madraspatana against Carbon tetrachloride Induced Hepatotoxicity. Research Journal of Pharmacognosy and Phytochemistry. 2014; 6(4): 176-180.  
9.    Ghawate VB, Shrivastava P, Bhambar RS. Hepatoprotective activity of Bridelia retusa bark extracts against carbon tetrachloride -induced liver damage in rats. Research Journal of Pharmacognosy and Phytochemistry. 2017; 9(2): 121-124
10.    Saleh I. Alqasoumi et al. Evaluation of Hepatoprotective effect of Aloe Vera, Clematis hirsute, Cucumis prophetarum and Bee Propolis against experimentally induced liver injury in rats. International Journal of Pharmacology. 2008; 4(3): 213-217.
11.    Leonard V Crowley. The Reitman-Frankel Colorimetric Transaminase Procedure in Suspected Myocardial Infarction. Clinical Chemistry, 1 June 1967; 13(6): 482–487.
12.    King EJ et al. King-Armstrong Phosphatase Estimation by the Determination of Liberated Phosphate. Journal of Clinical Pathology. 1951 Feb; 4(1): 85–91.
13.    Hansen PW. A simplification of Kind and King's method for determination of serum phosphatase. Scandinavian Journal of Clinical and Laboratory Investigation. 1966; 18(3): 353-356.
14.    Vaishya R. et al. Modification of Jaffe’s Kinetic Method decreases bilirubin interference: a preliminary report. Indian Journal of Clinical Biochemistry. 2010; 25(1): 64-66.
15.    Ohkawa H. et al. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry. Jun 1979; 95(2): 351-358.
16.    Davies MH et al. Direct Enzymatic Assay for Reduced and Oxidized Glutathione. Journal of Pharmacological Methods. 1984; 12: 191-194.
17.    Aebi, H. Catalase. In: Bergmeyer, H.U., Ed., Methods of Enzymatic Analysis, Verlag Chemie/Academic Press Inc., Weinheim/New York, 1974; 673-680.
18.    Marklund S. L. Human copper-containing superoxide dismutase of high molecular weight. Proceedings of the National Academy of Sciences of the United States of America. December 1, 1982; 79 (24): 7634-7638.
19.    Lowry OH et al. Protein Measurement with the Folin Phenol Reagent. Journal of Biological Chemistry. 1951; 193: 265-275.
20.    Samal PK. Hepatoprotective Activity of Gloriosa superba on Liver Damage Caused by CCl4 in Rats. Asian Journal of Pharmaceutical Research. 2013; 3(4): 194-197.
21.    Patel J, Reddy V, Kumar GS. Evaluation of hepatoprotective activity of ethanolic extract of Diospyros melanoxylon (Roxb) leaves against CCl4 induced hepatotoxicity in albino rats. Research Journal of Pharmacy and Technology. 2015; 8(5): 571-574.
22.    Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants. CSIR, New Delhi. 1959; p. 123.
23.    Venkatraman G. et al. A tetracyclic triterpinoid from Garuga pinnata. Phytochemistry. 1994: 36; 417- 419.
24.    Venkatraman G et al. Diarylheptanoids from Garuga pinnata. Phytochemistry. 1993: 33; 1221- 1225.
25.    Kuamwat RS, Mruthunjaya K, Gupta MK. Hepatoprotective effect of Gallic acid and Gallic acid Phytosome against Carbon Tetrachloride induced damage in albino rats. Research Journal of Pharmacy and Technology. 2012; 5(5): 677-681.
26.    Thirumalai T et al. Restorative effect of Eclipta alba in CCl4 induced hepatotoxicity in male albino rats. Asian Pacific Journal of Tropical Disease. 2011; 304-307.
27.    Kanchana N and Sadiq AM. Hepatoprotective effect of Plumbago zeylanica on paracetamol induced liver toxicity in rats. International Journal of Pharmacy and Pharmaceutical Sciences. 2011; 3(1): 151 -154.
28.    Venkatesh P, Hepcy Kalarani D, Dinakar A. Hepatoprotective Activity of an Aqueous Extract of Stem and Leaves of Boerhaavia diffusa Against Carbon Tetra Chloride Induced Hepatotoxicity in Rats. Research Journal of Pharmacy and Technology. 2010; 3(3): 840-842.
29.    Goel Ajay et al. Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in chlorpyrifosinduced toxicity. Chemico‐Biological Interactions. 2005; 156: 131–140.
30.    Patil SA et al. Hepatoprotective and Antioxidant Potential of Extracts from Whole Plant of Solanum surattense Burm. Research Journal of Pharmacognosy and Phytochemistry. 2011; 3(1): 17-21.
31.    Wu YH et al. Effect of Laggera alata on hepatocytes damage induced by carbon tetrachloride in vitro and in vivo. Journal of Ethnopharmacology. 2009; 126: 50–56.
32.    Shukla A, Bigoniya P. Hepatoprotective Effect of Lepidium Sativum Linn (Cruciferae) Total Alkaloid Fraction against CCl4 Induced Hepatotoxicity on Rats. Research Journal of Pharmacognosy and Phytochemistry. 2013; 5(2): 94-99.

Recomonded Articles:

Author(s): Nimbalkar V.V., Pansare P.M., Nishane B.B.

DOI: 10.5958/0974-360X.2015.00310.8         Access: Open Access Read More

Author(s): Trophimus Gnanabagyan Jayakaran, Vignesh R, Shankar P

DOI: 10.5958/0974-360X.2019.00700.5         Access: Open Access Read More

Author(s): Vijayalakshmi. S , Disalva. X, Chittaranjan Srivastava, Arun A

DOI: 10.5958/0974-360X.2019.00520.1         Access: Open Access Read More

Author(s): Muhammad Hamdan, Noorhamdani AS, Masruroh Rahayu, Mohammmad Hasan Machfoed

DOI: 10.5958/0974-360X.2019.01020.5         Access: Open Access Read More

Author(s): Hayat M. Mukhtar, Vandna Kalsi

DOI: 10.5958/0974-360X.2018.00395.5         Access: Open Access Read More

Author(s): Vaseeha Banu T.S., Sandhya K.V., K.N. Jayaveera

DOI: Not Available         Access: Open Access Read More

Author(s): Vandana Devi Sahu, Mukta Agrawal, Mukesh Sharma, Sandhya Chandrakar, Sujata Gupta, Garima Sharma, Kalyani Dewangan, Harsha Solanki, Manisha Majumdar, D. K. Tripathi, Amit Alexander, Ajazuddin

DOI: 10.5958/0974-360X.2016.00145.1         Access: Open Access Read More

Author(s): M. Sumithra, Avantika Prabhabanik

DOI: 10.5958/0974-360X.2017.00036.1         Access: Open Access Read More

Author(s): Raj Kumar R, Praveen D, Palani Shanmugasundaram

DOI: 10.5958/0974-360X.2016.00164.5         Access: Open Access Read More

Author(s): Pande S. D., Wagh A.S., Bhagure L.B., Patil S.G., Deshmukh A.R.

DOI: 10.5958/0974-360X.2015.00070.0         Access: Open Access Read More

Author(s): V Rajesh Babu, Syeda Rana Nikhat, P Nivethithai, SH Areefulla

DOI: Not Available         Access: Open Access Read More

Author(s): Loveleen Preet Kaur, Rajeev Garg, GD Gupta

DOI: Not Available         Access: Open Access Read More

Author(s): H.B. Pagar, U.P. Shinde, Y.S. Agrawal, S.D. Barhate, T.S. Luhade, R.O. Sonawane

DOI: Not Available         Access: Open Access Read More

Author(s): Barokar A.A., Wagh R.D., Baviskar D.T., Shaikh T.J.

DOI: Not Available         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags