Evaluation of Hepatoprotective activity of Ethanolic Extract of Garuga pinnata Roxburgh leaves against Carbon tetrachloride induced Hepatotoxicity in rats.
Sandeep Chavan1*, Remeth Dias2, Chandrakant Magdum3
1Assistant Professor, Department of Pharmacology, Tatyasaheb Kore College of Pharmacy, Warananagar,
Tal- Panhala, Dist- Kolhapur 416113 Maharashtra, India.
2Head of Pharmacy Department, Government Polytechnic Jalgaon, National Highway No. 6, Jalgaon,
Pin- 425001 Maharashtra, India.
3Professor and Principal, Rajarambapu College of Pharmacy, Kasegaon, Sangli, Pin- 415404 Maharashtra, India.
Volume - 14,
Issue - 5,
Year - 2021
In this study we investigated the in vivo Hepatoprotective activity of ethanolic extract of Garuga pinnata (EEGP) leaves in Carbon tetrachloride (CCl4) induced hepatotoxicity using wistar rats of either sex as model. Hepatotoxicity was induced by the administration of CCl4 intraperitoneally (0.125ml CCl4 in liquid paraffin (1:1) per 100g body weight). Garuga pinnata leaves extract at different dose levels (200 and 400mg/kg, p.o.) showed the dose dependant hepatoprotective effect and was compared with well known standard hepatoprotective Silymarain (100mg/kg). When groups were treated with CCl4, significant increase in serum biochemical parameters such as Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Glutamate Pyruvate Transaminase (SGPT), Alkaline phosphate (ALP), Acid Phosphate (ACP), Creatinine and alteration of tissue biochemical parameters such as reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (LPO), and the total proteins were observed. The histopathological examination of the CCl4 treated groups showed sinusoidal congestion, centrilobular necrosis, marked vacuolations and congestion. However, pretreatment with extract of leaves of Garuga pinnata significantly reduced the increased serum levels of biochemical parameters and restored antioxidant defense enzymes level to its normal. Moreover, histopathology of leaves extract treated groups showed normal architecture with minimal sinusoidal congestion. Taken together, our study concludes that EEGP to be a more potential agent for caring liver from CCl4 induced damage.
Cite this article:
Sandeep Chavan, Remeth Dias, Chandrakant Magdum. Evaluation of Hepatoprotective activity of Ethanolic Extract of Garuga pinnata Roxburgh leaves against Carbon tetrachloride induced Hepatotoxicity in rats. Research Journal of Pharmacy and Technology. 2021; 14(5):2375-0. doi: 10.52711/0974-360X.2021.00419
Sandeep Chavan, Remeth Dias, Chandrakant Magdum. Evaluation of Hepatoprotective activity of Ethanolic Extract of Garuga pinnata Roxburgh leaves against Carbon tetrachloride induced Hepatotoxicity in rats. Research Journal of Pharmacy and Technology. 2021; 14(5):2375-0. doi: 10.52711/0974-360X.2021.00419 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-5-3
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