Author(s): B. Srivastava, Sheeja V.K, Y. Haribabu, Ebin C.J

Email(s): sheejasureshsree@yahoo.com

DOI: 10.52711/0974-360X.2021.00439   

Address: B. Srivastava, Sheeja V.K*, Dr. Y. Haribabu, Ebin C.J
Department of Pharmaceutical Analysis, Grace College of Pharmacy, Palakkad, Kerala, 678004, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 5,     Year - 2021


ABSTRACT:
A selective, sensitive, rugged and high throughput high performance liquid chromatography tandem mass spectrometric method was developed for the simultaneous estimation of Emtricitabine and Tenofovir Alafenamide in Bulk and Tablet Dosage form. Liquid chromatography system has been coupled with tandem quadrupole mass spectroscopy with LC-20AD pump, electrospray ionization (ESI) interface, PDA detector (SPD-M20), column oven (CTO-20AC), CEM-20 alite controller and SIL-20AC auto sampler. Lab solution data station software was used. The separation of both Tenofovir and Emtricitabine was achieved by Gemini C18 column (4.6×100mm, 5µm) as a stationary phase with 0.1 % formic acid: methanol at the ratio of 20:80, v/v at a flow rate of 0.5ml/min and injection volume of 10 µl. The percentage Recovery of the Drug Tenofovir Alafenamide and Emtricitabine was 96.59 and 96.84% respectively. The linearity range was 20-120 ng/ml for both the drugs and the LOD was found to be 5 and 10 ng/ml and LOQ were 15 and 20 ng/ml for Tenofovir and Emtricitabine Respectively. The developed method was precise, accurate, sensitive and can be used for the routine quality control of the drug formulation.


Cite this article:
B. Srivastava, Sheeja V.K, Y. Haribabu, Ebin C.J. Method Development and Validation of Simultaneous Estimation of Emtricitabine and Tenofovir Alafenamide in Bulk and tablet Dosage form using LC-MS/MS. Research Journal of Pharmacy and Technology. 2021; 14(5):2493-6. doi: 10.52711/0974-360X.2021.00439

Cite(Electronic):
B. Srivastava, Sheeja V.K, Y. Haribabu, Ebin C.J. Method Development and Validation of Simultaneous Estimation of Emtricitabine and Tenofovir Alafenamide in Bulk and tablet Dosage form using LC-MS/MS. Research Journal of Pharmacy and Technology. 2021; 14(5):2493-6. doi: 10.52711/0974-360X.2021.00439   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-5-23


REFERENCE:
1.    Stephen Weller, Julie Borland, Shuguang Chen, Mark Johnson, Paul Savina, Brian Wynne, Toshihiro Wajima, Amanda F. Peppercorn, Stephen C. Piscitelli. European Journal of Clinical Pharmacology, 2014, 70; 29–35.
2.    Guidance for Industry, Bioanalytical Method Validation, U.S. Department of Health and Human Services, Food and Drug Administration Center for Drug Evaluation and Research, 2001.
3.    Bennetto-Hood C, Tabolt G, Savina P, Acosta EP. J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jan 15;945-946: 225-32.
4.    J. X. de Vries, A. Voss. Biomedical Chromatography, 1993, 7(1), 12-14
5.    Taomin Huang, Zhong He, Bei Yang, Luping Shao, Xiaowei Zheng, Gengli Duan. Journal of Pharmaceutical and Biomedical Analysis, 2006, 41(2), 644-648.
6.    Nevin Erk. Journal of Chromatography B, 2003, 784(1), 195-201.
7.    Fei Liu, Yu Xu, Shu Gao, Jundong zhangb, Qingxiang Guo , Journal of Pharmaceutical and Biomedical Analysis, 2007, 44(5), 1187-1191.
8.    Dragica Zendelovska, Trajče Stafilov, and Petar Miloševski. Biomedical Chromatography, 2004, 18(2), 71-76.
9.    Connie T. Azumaya. Journal of Chromatography B: Biomedical Sciences and Applications, 1990, 532, 168-174.
10.    N. V. S. Ramakrishna, K. N. Vishwottam, S. Manoj, M. Koteshwara, S. Wishu and D. P. Varma. Biomedical Chromatography, 2005, 19(10), 751–760.
11.    Hao Li, Yingwu Wang, Yao Jiang, Yunbiao Tang, Jiang Wang, Limei Zhao, Jingkai Gu. Journal of Chromatography B, 2007, 852(1-2), 436-442.
12.    Dongyang Liu, Ji Jiang, Peng Wang, Sheng Feng, Pei Hu, . Journal of Chromatography B, 2010, 878(9-10), 743-748.
13.    Sagar A. Parekh, Ashutosh Pudage, Santosh S. Joshi, Vikas V. Vaidya, Noel A. Gomes, Sudhir S. Kamat Journal of Chromatography B, 2008, 873(1), 59-69.
14.    Maha F. Tutunji , Husam M. Ibrahim, Manal H. Khabbas, Lara F. Tutunji . Journal of Chromatography B, 2009, 877(16-17), 1689-1697.
15.    Leena R. Bhata, Rahul K. Godgea, Asfak T. Voraa and Mrinalini C. Damlea. Journal of Liquid Chromatography and Related Technologies, 2007, 30(20), 3059-3067.
16.    Ritesh Sharma, Shyam Pancholi. Acta Pharmaceutica, 2012, 62( 1), 45-58.
17.    Samya M. El-Gizawy, Osama H. Abdelmageed, Mahmoud A. Omar, Sayed M. Deryea, Ahmed M. AbdelMegied. American Journal of Analytical Chemistry, 2012, 3(6), 422-430.
18.    D Vijaya Bharathi, Kishore Kumar Hotha, Pankaj K Chatki, Satyanarayana V, Venkateswarlu V. Bioanalysis, 4(10), 1195-1204.


Recomonded Articles:

Author(s): Niha Naveed, Karthikeyan Murthykumar, Subasree Soundarajan, Sripradha Srinivasan

DOI: Not Available         Access: Open Access Read More

Author(s): Umesh Kumar, Rakesh Narang, Surendra Kumar Nayak, Sachin Kumar Singh, Vivek Gupta

DOI: 10.5958/0974-360X.2017.00425.5         Access: Open Access Read More

Author(s): S. Subasree, Karthikeyan Murthykumar, Dhanraj

DOI: 10.5958/0974-360X.2016.00116.5         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Hayat M. Mukhtar, Vandna Kalsi

DOI: 10.5958/0974-360X.2018.00395.5         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj Charde, Rajesh Shukla, Rita M. Charde

DOI: Not Available         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): Pankaj Kumar, Jane Jacob, Khadjeeth Ajina, Jazeela, Fathima

DOI: 10.5958/0974-360X.2016.00101.3         Access: Open Access Read More

Author(s): Rituraj Singh Chundawat, Y.S. Sarangdevot, R.P.S. Rathore, Dharmendra Singh Sisodiya, Udaibhan Singh Rathore

DOI:         Access: Open Access Read More

Author(s): Anket Sharma, Vinod Kumar, Parminder Kaur, Ashwani Kumar Thukral, Renu Bhardwaj

DOI: 10.5958/0974-360X.2015.00299.1         Access: Open Access Read More

Author(s): Mahadeva Rao U.S., Suganya M. Utharkar, C. Shanmuga Sundaram

DOI: 10.5958/0974-360X.2015.00307.8         Access: Open Access Read More

Author(s): Oruganti Sai Koushik, V. Himaja, P. Srinivasa Babu, Ramadoss Karthikeyan

DOI: 10.5958/0974-360X.2015.00298.X         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags