Author(s): Sridatla V.V.S.S.N. Raju, S. Venkat Rao, A. Manikandan

Email(s): dvsraju70@gmail.com , svr.org@gmail.com

DOI: 10.52711/0974-360X.2021.00432   

Address: Sridatla V.V.S.S.N. Raju1*, S. Venkat Rao2, A. Manikandan1
1Bharath Institute of Higher Education and Research, Selaiyur, Chennai - 600073, India.
2Department of Chemistry, SMS Pharmaceuticals Ltd, R&D Centre (A DSIR Approved), Gagillapur, Telangana - 500047, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 5,     Year - 2021


ABSTRACT:
A delicate, fast, Accurate, exact and steadiness, showing isocratic RP-UPLC method was developed for the concurrent assurance of the Ceftazidime and Avibactam in bulk and formulation. To optimize, a column HSS C18 100 x 2.1mm, 1.8µm, Mobile phase containing water: acetonitrile choose in the ratio 75:25v/v was pumped through column at a flow rate of 0.3ml/min at 260nm, initiate to be an efficient method for elution of drug with good peak shapes as well as retention times. Rt of Avibactam and Ceftazidime were initiate to be 1.463 min and 2.109 min. %Recovery was obtained as 100.07% and 100.08% for Avibactam and Ceftazidime separately. LOD, LOQ values got from relapse conditions of Avibactam and Ceftazidime were 0.85, 2.56 and 3.53, 10.70 correspondingly. Regression equation of Ceftazidime is y = 7883.2x + 12277, and y = 3279.1x + 1137 of Avibactam.


Cite this article:
Sridatla V.V.S.S.N. Raju, S. Venkat Rao, A. Manikandan. Estimation of Ceftazidime and Avibactam in their Bulk and Formulations by a newly Developed and Validated of Stability Indicating RP-UPLC Method. Research Journal of Pharmacy and Technology. 2021; 14(5):2459-3. doi: 10.52711/0974-360X.2021.00432

Cite(Electronic):
Sridatla V.V.S.S.N. Raju, S. Venkat Rao, A. Manikandan. Estimation of Ceftazidime and Avibactam in their Bulk and Formulations by a newly Developed and Validated of Stability Indicating RP-UPLC Method. Research Journal of Pharmacy and Technology. 2021; 14(5):2459-3. doi: 10.52711/0974-360X.2021.00432   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-5-16


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2.    David Yuxin Wang, Martine I Abboud, Marios S Markoulides, Jürgen Brem and Christopher J Schofield. The road to avibactam: the first clinically useful non-β-lactam working somewhat like a β-lactam. Future medicinal chemistry.2016; 8(10).
3.    Hayes MV, Orr DC. Mode of action of ceftazidime: affinity for the penicillin-binding proteins of Escherichia coli K12, Pseudomonas aeruginosa and Staphylococcus aureus. J Antimicrob Chemother. 1983;12(2): 119-26.
4.    Syeda Saniya Fatima, R. Vani. Stability indicating analytical method development and validation for estimation of Ceftazidime and Avibactam in bulk and pharmaceutical dosage form using RP-HPLC. Int. J. of Farmacia, 2016; Vol-(2) 1: 70-78.
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