Author(s): Monica Joicy. C, Sivaraj. C, Arumugam. P

Email(s): shivaraj27@gmail.com

DOI: 10.52711/0974-360X.2021.00431   

Address: Monica Joicy. C1, Sivaraj. C2, Arumugam. P3
1Queen Maris College, Chennai - 600004, Tamilnadu, India.
2ARMATS Biotek Training and Research Institute, Chennai – 600032, Tamilnadu, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 5,     Year - 2021


ABSTRACT:
Illicium verum is a small native evergreen tree of northeast Vietnam, India and southwest China. It is the flower of a medium-sized tree and is also known as star anise belongs to the family of Schisandraceae that grows in North America, Atlantic region, and tropical and subtropical zones of Asia. This study investigated the in vitro cytotoxic effects of essential oil obtained from the flowers of Illicium verum is also known as star anise. Steam distillation method was used to extract oil from the flowers of Illicium verum. Antioxidant activities such as DPPH (2,2-diphenyl-1-picrylhydrazyl), and ferric reducing power assays were assessed followed by total phenolic contents was estimated by folin-ciocalteau reagent method. Gas chromatography–mass spectrometry (GC-MS) analysis was used to find out volatile and semi-volatile compounds of essential oil extracted from Illicium verum. Anti-proliferative effect was studied by MTT assay method using MCF 7 and HepG 2 cancer cell lines. The maximum HepG 2 cell death was 25.73 ±0.24 % at 100µg/mL concentration and the IC50 was 194.32±0.24% at 100µg/mL concentration. The maximum MCF 7 cell death was 32.64±0.32% at 100µg/mL concentration and the IC50 was 153.18 ± 0.32% at 100µg/mL concentration.


Cite this article:
Monica Joicy. C, Sivaraj. C, Arumugam. P. In-vitro Antioxidant, Antidiabetic, Antibacterial and Cytotoxic Activities of Essential Oil extracted from Flowers of Illicium verum L. Research Journal of Pharmacy and Technology. 2021; 14(5):2452-8. doi: 10.52711/0974-360X.2021.00431

Cite(Electronic):
Monica Joicy. C, Sivaraj. C, Arumugam. P. In-vitro Antioxidant, Antidiabetic, Antibacterial and Cytotoxic Activities of Essential Oil extracted from Flowers of Illicium verum L. Research Journal of Pharmacy and Technology. 2021; 14(5):2452-8. doi: 10.52711/0974-360X.2021.00431   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-5-15


REFERENCES:
1.    Hanahan. D and Weinberg R. A, “Hallmarks of cancer: the next generation,” Cell, vol. 144, no. 5, pp. 646–674, 2011.
2.    Muhammad Asif D, Ashwaq Hamid Salem Yehya B, Majed Ahmed Al-Mansoub. A, Vageesh Revadigar, Mohammed Oday Ezzat, Mohamed B. Khadeer Ahamed, Chern Ein Oonb, Vikneswaran Murugaiyah, Aman Shah Abdul Majid, Amin Malik Shah Abdul Majid, Anticancer attributes of Illicium verum essential oils against colon cancer. Vol.103, pp.156–161, 2016
3.    Pita J. C. L. R.,.Xavier A. L, De Sousa T. K. G., “In vitro and in vivo antitumor effect of trachylobane-360, a diterpene from Xylopia langsdorffiana,” Molecules, vol. 17, no. 8, pp. 9573–9589, 2012.
4.    Efferth T., “Cancer therapywith natural products and medicinal plants,” Planta Medica, vol. 76, pp. 1035–1036, 2010.
5.    De Sousa D. P., Medicinal Essential Oils: Chemical, Pharmacological and Therapeutic Aspects, Nova Science Publishers, New York, NY,USA, 1st edition, 2012.
6.    Rasoanaivo P., Randriana R. F.,. Maggi F, “Chemical composition and biological activities of the essential oil of Athanasia brownie Hochr. (Asteraceae) endemic to Madagascar,” Chemistry & Biodiversity, vol. 10, no. 10, pp. 1876–1886, 2013.
7.    Zapata B., Betancur L. G., Duran C., and Stashenko E., “Cytotoxic activity of Asteraceae and Verbenaceae family essential oils,” Journal of Essential Oil Research, vol. 6, pp. 50–57, 2014.
8.    Piaru S. P., Mahmud.R., Majid A. M. S. A., Ismail.S., and Man C. N., “Chemical composition, antioxidant and cytotoxicity activities of the essential oils of Myristica fragrans and Morinda citrifolia,” Journal of the Science of Food and Agriculture, vol. 92, pp. 593–597, 2012.
9.    Ferraz R. P. C., Bomfim D. S., Carvalho N. C., Da Silva T.B, Machado W.J, Prata A.P, Costa E.V, Moraes V.R, Nogueira P.C, Bezerra D.P., “Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae),” Phytomedicine, vol. 20, pp. 615–621, 2013.
10.    Maggi F. F., Randriana R. F, Rasoanaivo P., “Chemical composition and in vitro biological activities of the essential oil  of Vepris macrophylla (BAKER) I.VERD. endemic to Madagascar,”Chemistry & Biodiversity, vol. 10, no. 3, pp. 356–365, 2013.
11.    Adorjan, B., Buchbauer, G., 2010. Biological properties of essential oils: an updated review.Flavour Fragance J. 25, 407–426.
12.    Aisha, A.F.A., Abu-Salah, K.M., Ismail, Z., Majid, A.M.S.A., 2012. In vitro and in vivo anticolon cancer effects of Garcinia mangostana xanthones extract. BMC Complement. Altern. Med. 12, 104.
13.    Akrout, A., Gonzalez, L.A., Jani, H.E., Madrid, P.C., 2011. Antioxidant and antitumor activities of Artemisia campestris and Thmelaea hirsuta from southern Tunisia. Food Chem. Toxicol. 49, 342–347.
14.    Al-Harbi, M.M., Qureshi, S., Raza, M., Ahmed, M.M., Giangreco, A.B., Shah, A.H., 1995. Influence of anethole treatment on the tumour induced by Ehrlich ascites carcinoma cells in paw of Swiss albino mice. Eur. J. Cancer Prev. 4, 307–318.
15.    Al-Mansoub, M.A., Asmawi, M.Z., Murugaiyah, V., 2014. Effect of extraction solvents and plant parts used on the antihyperlipidemic and antioxidant effects of Garcinia. atroviridis: a comparative study. J. Sci. Food Agric. 94, 1552–1558.
16.    Amin, A., Gali-Muhtasib, H., Ocker, M., Schneider-Stock, R., 2009. Overview of major classes of plant-derived anticancer drugs. Int. J. Biol. Sci. 11, 1–11.
17.    Asif, M., Jabeen, Q., Abdul Majid, A.M.S., Atif,M., 2014. Diuretic activity of Boswellia serrata Roxb. oleo gum extract in albino rats. Pak. J. Pharm. Sci. 27, 1811–1817.
18.    Benzie, I.F., Strain, J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of ‘antioxidant power’: the FRAP assay. Anal. Biochem. 239, 70–76.
19.    Bhall, Y., Gupta, V.K., Jaitak, V., 2013. Anticancer activity of essential oils: a review. J. Sci. Food Agric. 93, 3643–3653.
20.    Cai, Y., Luo, Q., Sun, M., Corke, H., 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 74, 2157–2184.
21.    Carroll, R.E., Benya, R.V., Turgeon, D.K., Vareed, S., Neuman, M., Rodriguez, L., Kakarala, M.,Carpenter, P.M., McLaren, C., Meyskens Jr., F.L., Brenner, D.E., 2011. Phase IIa clinical trial of curcumin for the prevention of colorectal neoplasia. Cancer Prev. Res.(Phila). 4, 354–364.
22.    Carvalho, A.A., Andrade, L.N., Sousa, É.B.Vd., Sousa, D.Pd., 2015. Antitumor phenylpropanoids found in essential oils. BioMed. Res. Int. 2015, 1–21.
23.    Cha, J.D., Moon, S.E., Kim, H.Y., Cha, I.H., Lee, K.Y., 2009. Essential oil of Artemisia capillaries induces apoptosis in KB cells via mitochondrial stress and caspase activation mediated by MAPK-stimulated signaling pathway. J. Food Sci. 74, 75–81.
24.    Cha, J.D., Kim, Y.H., Kim, J.Y., 2010. Essential oil and 1,8-cineole from Artemisia lavandulaefolia induces apoptosis in KB cells via mitochondrial stress and caspase activation. Food Sci. Biotechnol. 19, 185–191.
25.    Hossan SJ, El-Sayed M, Aoshima H. Antioxidative and anti α-amylase activities of four wild plants consumed by nomads in Egypt. Orient Pharm Exp Med. 2009; 9(3): 217-224.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags