Subhajit Ghosh, Padala Narasimha Murthy, Hanumanthachar Joshi
Subhajit Ghosh1*, Padala Narasimha Murthy2, Hanumanthachar Joshi1
1Sarada Vilas College of Pharmacy, Mysore-570004, Karnatata, India.
2Royal College of Pharmacy and Health Sciences, Andhapasara Road, Berampur-760002, Odisha, India.
Volume - 14,
Issue - 5,
Year - 2021
Kashayam are unique Ayurvedic Formulation, most of them Polyherbal oral dosage form, used as a medicinal rationale. One of them Kokilaksha Kashayam, Quality of Kokilaksha Kashayam depends only on quality of starting materials, processing of ingredients, meticulous crushing, heating cycle.In traditional system of medicine like Ayurveda, Kokilaksha Kashayam is one of the traditional Indian medicine which is a polyherbal preparation treated with plant extract. It is generally used in the treatments of disorders related to Anti-inflammatory, Analgesic,heart, cancer etc. however detailed characterization studies after preparation & comparison with marketed formulation are significant which can express authenticity of the product. Here the research work deals with the preparation of Kokilaksha Kashayam as per the procedure mentioned in the Ayurvedic text and modern method of preparation. Prepared Kokilaksha Kashayam was characterized and identified by different Analytical techniques, then evaluate preclinical pharmacological studies and also compared with Marketed formulations. Special steps concerned in the preparation of Kokilaksha Kashayam include Upakara (Preparation), Jaran (Heating & Stirring), Shoshan (Purification), Dravyaguna parlksa (Pharmacological experiments), tulana parlksa (comparison Study) by traditional or conventional as well as modern methods of preparation. These products obtained from Jaran (Heating & Stirring), Shoshan (Purification) by water extraction (Decoction process) and marketable sample were analyzed for quality control checks, the parameters mentioned in Ayurvedic texts as well as in modern techniques of evaluation, and pre-clinical pharmacological studies such as Assay of lipid per- oxidation, Hypolipidemic Activity etc were done to find out nature and form of the prepared formulation. Analyzed the in-vitro with in-vivo Anti-hyperlipidemic activity, bio-accessibility of Kokilaksha Kashayam were also determined. The Study reveals that the prepared Kokilaksha Kashayam-T and Kokilaksha Kashayam-M was shown the antioxidant activity was expanded in focus subordinate way. Kokilaksha Kashayam -T and Kokilaksha Kashayam-M repressed the ferrous sulfate prompted lipid per-oxidation in a measurements subordinate way and demonstrated inhibitory focus (IC50) esteem 166.173 and 170.284 µg/ml individually observed from graphical representation. By Oral Administration of Kokilaksha Kashayam-T and Kokilaksha Kashayam-M for nine weeks at the measurement of 2 ml/kg fundamentally decreased serum cholesterol, serum LDL and serum triglycerides while indicated critical ascent in serum HDL when contrasted with high fat eating routine encouraged control gathering. Marketed Kokilaksha Kashayam additionally demonstrated critical decline in serum cholesterol, serum LDL, serum triglycerides and indicated noteworthy ascent in serum HDL. Atorvastatin (1.2 mg/kg, p.o.) was utilized as standard antihyperlipidemic drug.The Study confirmed prepared two kinds of Kokilaksha Kashayam as Kokilaksha Kashayam-T and Kokilaksha Kashayam-M and Compared with Marketed Formulation indicated noteworthy decrease in atherogenic record when contrasted with high fat eating routine bolstered control amass which emphatically underpins antiatherosclerotic property of Kokilaksha Kashayam. Hence, it is concluded that Kokilaksha Kashayam can be very useful as cardiac disorder and extending the freshly prepared more active than Marketed formulation.
Cite this article:
Subhajit Ghosh, Padala Narasimha Murthy, Hanumanthachar Joshi. Different Methods of Preparation, Evaluation and Comparison of One Traditional Oral Liquid Formulation for Potential Antihyperlipidemic Activity in Hyperlipidemic Wistar Rats. Research Journal of Pharmacy and Technology. 2021; 14(5):2426-3. doi: 10.52711/0974-360X.2021.00427
Subhajit Ghosh, Padala Narasimha Murthy, Hanumanthachar Joshi. Different Methods of Preparation, Evaluation and Comparison of One Traditional Oral Liquid Formulation for Potential Antihyperlipidemic Activity in Hyperlipidemic Wistar Rats. Research Journal of Pharmacy and Technology. 2021; 14(5):2426-3. doi: 10.52711/0974-360X.2021.00427 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-5-11
1. Tripathi. K. D. Essentials of Medical Pharmacology. 4th Edn, published by Jaypee Brothers, New Delhi, 2002; 612-614.
2. Singh N, Kapur KK and Singh SP. Mechanism of cardiovascular action of Terminalia arjuna. J Med Plant Res. 1982; 45:102-104.
3. The Ayurvedic Formulary of India Part-I. Delhi, India: Controller of Publications; 2000. p. 15-16.
4. Baydar NG, Ozkan G, Sagdic O. Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control 2004; 15:335-339.
5. Frankel EN, Kanner J, German JB, Parks E, Kinsella JE. Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. The Lancet 1993; 341(20): 454-457.
6. Mayer AS, Yi OS, Person DA, Waterhouse DL, Frankel EN. Inhibition of human low-density lipoprotein oxidation in relation to composition of phenolic antioxidants in grapes (Vitis vinifera). J Agri Food Chem 1997; 45:1638-1643.
7. Teissedre PL, Frankel EN, Waterhouse AL, Peleg H, German GB. Inhibition of in vitro human LDL oxidation by phenolic antioxidants from grapes and wines. J Sci Food Agri 1996; 70: 55- 61.
8. Waterhouse AL. Wine antioxidants may reduce heart disease and cancer. Presentation of American Chemical Society, Washington; 1994
9. Mishra S. Bhaisazya Kalpana Vigyan. Varanasi, India: Chaukambha Surbharati Prakashan; 2005. p. 253-254.
10. Alam M, Radhamani S, Ali U, Purushottam KK. Microbiological Screening of Dhataki Flowers. J Res Ayurveda Siddha. 1984; 2(4):371-375.
11. Braugghler JM, Duncan CA and Chase IR. The involvement of iron in lipid peroxidation. J Biol Chem. 1986; 261(22): 10282-89.
12. Lowery OH, Rosenbrough NJ, Farr AL and Randall RJ. Protein estimation with Folin phenol reagent. Biol Chem. 1951; 193:265- 275.
13. Khanna AK, Chander R and Kapoor NK. Terminala arjuna: an Ayurvedic cardiotonic, Regulates lipid metabolism in Hyperlipidemic rats. Phytother Res. 1996;10: 663-665
14. Allain CC, Poon LS, Chan CS and Richmond W. Enzymatic Determination of Total Serum cholesterol. Clin Chem. 1974; 20::447-475.
15. Friedewald WT, Levy RI and Fredrickson DS. Estimation of concentration of low density cholesterol in plasma without use of ultracentrifuge. J Clin Chem.1972;18: 449-502.
16. Muller PH, Schmulling RM, Liebich HM and Eggstein M. A fully Enzymatic Triglyceride Determination. J Clin Chem. 1977;15:457- 464.
17. Anne SM, Ock SY, Debra AP, Andrew LW and Edwin NF. Inhibition of Human Low Density Lipoprotein oxidation in relation to composition of Phenolic antioxidants in Grapes (Vitis vinifera). J Agri Food Chem. 1997;45 (5):1638-1643
18. S Renaud, and M De Lorgeril. Wine, alcohol, Platelets and the French Paradox for Coronary Heart Disease. The Lancet. 1992; 339: 1523-1526
19. Pederson TR. Low- density lipoprotein cholesterol lowering is and will be the key to the future of lipid management. American J Cardiol. 2001; 87(5A): 8B-12B.
20. Boden WE and Pearson TA. Raising low levels of High Density Lipoprotein Cholesterol is an important target of therapy. American J Cardiol. 2000;