Noha Ibrahim, Eman S. Elzanfaly, Ahmed E. El Gendy, Said A. Hassan
Noha Ibrahim1, Eman S. Elzanfaly2, Ahmed E. El Gendy1, Said A. Hassan2*
1Analytical Chemistry Department, Faculty of Pharmacy, MISR International University, Cairo, Egypt.
2Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Volume - 14,
Issue - 4,
Year - 2021
Green chemistry is an emerging field concerned with safe practices in chemical method development. The purpose of the study is to develop a sensitive, environmentally friendly spectrofluorimetric method by studying and optimizing variables affecting the native fluorescence intensity of Moxifloxacin using experimental design approach. The analysis was divided into three phases: studying and screening the critical factors using 2-level full factorial design, optimization using central composite design and validation according to International Conference on Harmonization (ICH) guidelines. The optimal experimental conditions obtained from this study were 0.05 M phosphate buffer with an adjusted buffer pH of 9.7 and a temperature of 7.4°C±0.5, providing a sensitive measurement of MOX. The validated method providing to be linear over a range of 5-40ng/mL (r= 0.9999), precise, accurate, and robust (demonstrated by the Plackett-Burman design). It can be concluded that the validated method can be safe, optimum, and environmentally friendly alternative for the analysis of MOX.
Cite this article:
Noha Ibrahim, Eman S. Elzanfaly, Ahmed E. El Gendy, Said A. Hassan. Development, Optimization, and Validation of a Green Spectrofluorimetric method for the determination of Moxifloxacin using an Experimental design approach. Research Journal of Pharmacy and Technology. 2021; 14(4):1880-6. doi: 10.52711/0974-360X.2021.00332
Noha Ibrahim, Eman S. Elzanfaly, Ahmed E. El Gendy, Said A. Hassan. Development, Optimization, and Validation of a Green Spectrofluorimetric method for the determination of Moxifloxacin using an Experimental design approach. Research Journal of Pharmacy and Technology. 2021; 14(4):1880-6. doi: 10.52711/0974-360X.2021.00332 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-4-8
1. Patel PM, Patil AA, Patil MD, Patil PS, Borse SL. Green chemistry -An overview. Asian J. Research Chem. 2013; 6(7): 705-709.
2. Matlack AS, Introduction to green chemistry. CRC Press, 2010.
3. Thangavelu C, Raymond PP, Rajendran S, Sundaravadivelu M. Sustainability of potable water system: toward green chemistry and green technologies. Asian J. Research Chem. 2011; 4(7):1033-1037.
4. Gupta SP, Upmanyu N, Garg G. Rising importance of green chemistry in India: A study. Research J. Pharm. and Tech. 2010; 3(2): 627-628.
5. Sharma S, Bansal T, Radhika, Kaur S, Jyoti. Green chemistry: An overview. Asian J. Research Chem. 2013; 6(11): 1075-1084.
6. Guillory JK. The Merck Index: An Encyclopedia of Chemicals, Drugs, and Biologicals. John Wiley and Sons, Hoboken, 2006, p.78.
7. Brunton LL, Chabner BA and Knollmann BC. Goodman and Gilman's The Pharmacological Basis of Therapeutics, McGraw-Hill, New York, 2011.
8. Warner IM, Patonay G, Rollie ME, Thomas M, Nelson G. Optimization of fluorescence measurments. In Progress in analytical luminescence, Edited by Eastwood D and Love LC. American society for testing and materials. Philadelphia.1988; pp 1-11.
9. Hassan SA, Ahmed SA, Helmy AH, Youssef NF. Spectrofluorimetric study on fluorescence quenching of tyrosine and l ‐tryptophan by the aniracetam cognition enhancer drug: quenching mechanism using Stern–Volmer and double‐log plots. Luminescence. 2020; 35 (5): 728–737.
10. Fisher R.A. The arrangement of field experiments. In Breakthroughs in statistics. Springer, New York, 1992; pp 82-91.
11. Youden WJ. The Fisherian revolution in methods of experimentation. Journal of the American Statistical Association. 1951; 46 (253): 47–50.
12. Wold S. Chemometrics, why, what and where to next?. Journal of Pharmaceutical and Biomedical Analysis. 1991; 9(8): 589-96.
13. Dalvi SD, Nanda RK, Chitlange SS. Full Factorial design for optimization, development, validation of RPHPLC method and stability-indicating method for tamsulosin and dutastaride. Asian Journal of Research in Chemistry. 2017; 10(4):504-512.
14. Amina BB, Roukia H, Mahfoud HA, Ahlem T, Sabrina B, Chahrazed B, Houria M. Optimization of extraction conditions of the polyphenols, flavonoids and the antioxidant activity of the plant Ammosperma cinereum (Brassicaceae) through the response surface methodology (RSM). Asian Journal of Research in Chemistry. 2020;13(1):01-06.
15. Mittal A, Parmar S, Gilani SJ, Imam SS, Taleuzzaman M. Optimization and validation for simultaneous estimation of citicoline and piracetam in bulk and tablet formulations using RP-HPLC method: Analytical quality by design approach. Asian Journal of Research in Chemistry. 2017; 10(2):198-205.
16. Abou‐Taleb NH, El‐Wasseef DR, El‐Sherbiny DT, El‐Ashry SMJL. Optimizing the spectrofluorimetric determination of cefdinir through a Taguchi experimental design approach. Luminescence. 2016; 31 (3) 856-864.
17. Ibrahim N, Elzanfaly ES, Elgendy AE, Hassan SA. Quality by design approach for the simultaneous determination of amlodipine and Olmesartan using HPLC with fluorescence detection. Pharmaceutical Chemistry Journal. 2020. (In press).
18. Ocaña JA, Barragán FJ, Callejón M. Spectrofluorimetric determination of moxifloxacin in tablets, human urine and serum. Analyst. 2000;125 (12): 2322-2325.
19. Kamruzzaman M, Alam AM, Lee SH, Ragupathy D, Kim YH, Park SR, Kim SH. Spectrofluorimetric study of the interaction between europium (III) and moxifloxacin in micellar solution and its analytical application, Spectrochim. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2012, 86, 375.
20. Shah J, Jan MR, Ullah I, Shah S. Sensitive spectrofluorimetric method for determination of fluoroquinolones through charge-transfer complex formation. American Journal of Analytical Chemistry. 2013; 4(10): 521.
21. Patel SH, Raval MA, Shah UM. Development and validation of spectrofluorometric method for the estimation of moxifloxacin in pharmaceutical dosage form. International Journal of Pharmacy and Pharmaceutical Sciences. 2013; 5(4): 252-54.
22. Chintawar PP, Pawar PN, Harde MT, Joshi SV, Chaudhari PD. Spectrophotometric methods for simultaneous estimation of moxifloxacin HCl and ketorolac tromethamine. Asian Journal of Research in Chemistry. 2010; 3(3):767-771.
23. Seethamma M, Vijayakumar B, Sai Prasad T, Venkateshwarlu G. Extractive spectrophotometric methods for determination of moxifloxacin hydrochloride using acidic triphenylmethane dyes. Asian Journal of Research in Chemistry. 2011; 4(8): 1297-1301.
24. Stass H, Dalhoff A. Determination of BAY 12-8039, a new 8-methoxyquinolone, in human body fluids by high-performance liquid chromatography with fluorescence detection using on-column focusing. Journal of Chromatography B: Biomedical Sciences and Applications. 1997; 702(1-2): 163-74.
25. Patel D, Patel M, Patel K. Simultaneous RP-HPLC estimation of moxifloxacin hydrochloride and ketorolac tromethamine in ophthalmic dosage forms. Asian Journal of Research in Chemistry. 2012; 5(5): 697-699.
26. Ba BB, Etienne R, Ducint D, Quentin C, Saux MC. Determination of moxifloxacin in growth media by high-performance liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications. 2001;754(1): 107-12.
27. Patel HP, Akhtar J. RP-HPLC method for the simultaneous estimation of moxifloxacin hydrochloride and cefixime trihydrate in synthetic mixture. Asian J. Research Chem. 2013; 6(5): 498-501.
28. Nguyen HA, Grellet J, Ba BB, Quentin C, Saux MC. Simultaneous determination of levofloxacin, gatifloxacin and moxifloxacin in serum by liquid chromatography with column switching. Journal of Chromatography B. 2004; 810(1): 77-83.
29. Khan GJ, Trivedi C, Soni K, Khan IJ, Namjoshi DR, Saraf MN. Determination of moxifloxacin in plasma by RP HPLC with fluorescence detection for bioequivalence studies in healthy human subjects. Indian Drugs-Bombay. 2005; 42(6): 375.
30. Laban-Djurdjević A, Jelikić-Stankov M, Djurdjević P. Optimization and validation of the direct HPLC method for the determination of moxifloxacin in plasma. Journal of Chromatography B. 2006; 844(1): 104-11.
31. Ulu ST. High-performance liquid chromatography assay for moxifloxacin: pharmacokinetics in human plasma. Journal of pharmaceutical and biomedical analysis. 2007; 43(1):320-24.
32. Kumar AH, Ramachandran G. Simple and rapid liquid chromatography method for determination of moxifloxacin in plasma. Journal of Chromatography B. 2009; 877(11-12): 1205-08.
33. ICH (Q1 R1) Validation of Analytical Procedures: Text and Methodology, In proceedings of International Conference on Harmonization, Geneva, 1996.
34. Telford JK. A brief introduction to design of experiments. Johns Hopkins Apl Technical Digest. 2007; 27(3): 224-32.
35. Williams RT, Bridges JW. Fluorescence of solutions: A review. Journal of clinical pathology. 1964; 17(4):371.
36. Montgomery DC. Design and analysis of experiments. John wiley and sons, Hoboken, 2017.
37. Hargis LG, Analytical chemistry: principles and techniques. Prentice Hall College Book Division, Unites states, 1988.