Author(s): Solomon Yeabyo, Zenebe Teka M, Gopalakrishnan V. K, Kamalakararao K, Muthulingam M, Krishna Chaithanya K

Email(s): krishanchaitanyawc@gmail.com

DOI: 10.52711/0974-360X.2021.00400   

Address: Solomon Yeabyo1, Zenebe Teka M2, Gopalakrishnan V. K1, Kamalakararao K3, Muthulingam M4, Krishna Chaithanya K1*
1Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Ethiopia.
2Departments of Biology, College of Natural and Computational Sciences, Aksum University, Axum, Ethiopia.
3Departments of Biochemistry, Adikavi Nannaya University, Rajahmundry, Andhra Pradesh, India.
4Department of Zoology, Faculty of Science, Annamalai University, Chidambaram, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 4,     Year - 2021


ABSTRACT:
Objective: The present study was aimed to evaluate the in vivo immune protective potential of chloroform root extract of Sida schimperiana on E.coli 018:K1 induced peritonitis in albino Wistar rats. Methods: Acute toxicity of was performed by oral administration of S. schimperiana chloroform root extract (SSRCH) 5, 50, 300 and 2000mg/kg body of male albino Wistar and mortality was monitored for 14 days. Based on LD50, 1/10th, 1/5th cut-off values of the (SSRCH) plant extract was selected as a dose for E. coli induced peritonitis in albino Wistar rats. Wistar rats were pre-treated with 200 and 400mg/kg/bwt of SSRCH and Standard antibiotic Ofloxacin 5mg/Kg body weight was given oraly for a period of 7 days. The dosing regimens were started on day -1,-2, -3, 0, 1, 2 and 3 relative to the day of challenge (day 0) with 2×104 CFU of E. coli CFU/ml (i.p.) and mortality was monitored for 14 days. After the monitoring the mortality, the treated (Groups I-IV) rats were sacrificed, and assess the in vivo antibacterial activity of S. schimperiana chloroform root extract by determination of CFU/ml in peritoneal lavage fluid. Further SSRCH extract (400mg /kg bw) was analyzed by the neutrophil adhesion in Wistar rats for evaluated immunomodulatory activity. Results: In acute toxicity studies no mortality was observed for 24-48 hours. SSRCH extract 400mg (Group-IV) showed protection against E. coli induced peritonitis in albino Wistar rats by showing 60% survivability and also exhibited significantly increased percentage of neutrophils adhesion. i.e (25.74±2.351and 36.14±5.609) at 200 and 400mg/kg respectively. Conclusion: The present study concluded that the chloroform root extract of S. schimperiana had a significant amount of polyphenolic compounds and could serve as a potential source of natural antibacterial and Immunomodulatory agents for the development of therapeutic antibiotics with immunostimulatory activity in the treatment of intraperitoneal infections.


Cite this article:
Solomon Yeabyo, Zenebe Teka M, Gopalakrishnan V. K, Kamalakararao K, Muthulingam M, Krishna Chaithanya K. Immuno protective potential of Sida schimperiana chloroform root extract against E. coli 018: K1 induced peritonitis in albino Wistar rats. Research Journal of Pharmacy and Technology. 2021; 14(4):2262-9. doi: 10.52711/0974-360X.2021.00400

Cite(Electronic):
Solomon Yeabyo, Zenebe Teka M, Gopalakrishnan V. K, Kamalakararao K, Muthulingam M, Krishna Chaithanya K. Immuno protective potential of Sida schimperiana chloroform root extract against E. coli 018: K1 induced peritonitis in albino Wistar rats. Research Journal of Pharmacy and Technology. 2021; 14(4):2262-9. doi: 10.52711/0974-360X.2021.00400   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-4-76


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