Author(s): Md. Tanvir Kabir, Mayesha Samiha, Hasina Yasmin, Mohammed S. Rahman, Md. Sohanur Rahman, Ghulam Md Ashraf, Raushanara Akter

Email(s): tanvir_kbr@yahoo.com , tanvir.kabir@bracu.ac.bd

DOI: 10.52711/0974-360X.2021.00391   

Address: Md. Tanvir Kabir1*, Mayesha Samiha1, Hasina Yasmin1, Mohammed S. Rahman2, Md. Sohanur Rahman3, Ghulam Md Ashraf4,5, Raushanara Akter1
1Department of Pharmacy, Brac University, 66 Mohakhali, Dhaka 1212, Bangladesh.
2Department of Pharmacy, Daffodil International University, 102 Mirpur Road, Dhaka 1207, Bangladesh.
3Department of Biochemistry and Molecular Biology, Trust University, Barishal, Ruiya, Nobogram Road, Barishal-8200, Bangladesh.
4King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
5Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
*Corresponding Author

Published In:   Volume - 14,      Issue - 4,     Year - 2021


ABSTRACT:
Crassocephalum crepidioides belongs to the Asteraceae family and the leaves of the plant were used in the phytochemical screening. The objective of this study was phytochemical analysis of C. crepidioides in order to evaluate its antioxidant, thrombolytic and cytotoxic effects. In this study, DPPH assay and total phenolic content (TPC) were used to evaluate the antioxidant property. Brine shrimp lethality test was performed to find the cytotoxicity. The TPC of methanolic extract of C. crepidioides was 637.22 mg/gm and gallic acid was standard in this procedure. The IC50 value of the plant extract of DPPH assay was 136.016 µg/mL obtained by using ascorbic acid as reference standard. Furthermore, the obtained IC50 value of ascorbic acid was 94.12 µg/mL. In this study, lethal concentrations of vincristine sulfate and the plant extract were 3.064 µg/mL and 69.245 µg/mL respectively. Indeed, the plant exhibited significant antioxidant, cytotoxic and thrombolytic activity.


Cite this article:
Md. Tanvir Kabir, Mayesha Samiha, Hasina Yasmin, Mohammed S. Rahman, Md. Sohanur Rahman, Ghulam Md Ashraf, Raushanara Akter. Free radical scavenging, Thrombolytic and cytotoxic effects of the medicinal herb, Crassocephalum crepidioides. Research Journal of Pharmacy and Technology. 2021; 14(4):2205-0. doi: 10.52711/0974-360X.2021.00391

Cite(Electronic):
Md. Tanvir Kabir, Mayesha Samiha, Hasina Yasmin, Mohammed S. Rahman, Md. Sohanur Rahman, Ghulam Md Ashraf, Raushanara Akter. Free radical scavenging, Thrombolytic and cytotoxic effects of the medicinal herb, Crassocephalum crepidioides. Research Journal of Pharmacy and Technology. 2021; 14(4):2205-0. doi: 10.52711/0974-360X.2021.00391   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-4-67


REFERENCES:
1. Senthilkumar, R.; Ahmedjohn, S.; Archunan, G.; Manoharan, N. Antioxidant activity of Wedelia chinensis in alloxan induced diabetic rats. Pharmacol. 2008, 640–651.
2. Yuan, H.; Ma, Q.; Ye, L.; Piao, G. The Traditional Medicine and Modern Medicine from Natural Products. Molecules 2016, 21.
3. Saha, S.; Hossain, F.; Anisuzzman, M.; Islam, M.K. Pharmacological Evaluation of MUSA Seminifera Lour. fruit. J. Chinese Integr. Med. 2013, 11, 253–261.
4. Sharmin, S.; Kabir, M.T.; Islam, M.N.; Jamiruddin, M.R.; Rahman, I.; Rahman, A.; Hossain, M. Evaluation of antioxidant, Thrombolytic and Cytotoxic Potentials of Methanolic Extract of Aporosa Wallichii Hook. f. leaves: An Unexplored Phytomedicine. J. Appl. Pharm. Sci. 2018, 8.
5. Uddin, M.S.; Al Mamun, A.; Kabir, M.T.; Jakaria, M.; Mathew, B.; Barreto, G.E.; Ashraf, G.M. Nootropic and Anti-Alzheimer’s Actions of Medicinal Plants: Molecular Insight into Therapeutic Potential to Alleviate Alzheimer’s Neuropathology. Mol. Neurobiol. 2019, 56, 4925–4944.
6. Herbal Drugs: Ethnomedicine to Modern Medicine; Springer Berlin Heidelberg, 2009.
7. Hossain, M.S.; Uddin, M.S.; Kabir, M.T.; Begum, M.M.; Koushal, P.; Herrera-Calderon, O.; Akter, R.; Asaduzzaman, M.; Abdel-Daim, M.M. In vitro Screening for Phytochemicals and Antioxidant Activities of Syngonium Podophyllum L.: An Incredible Therapeutic Plant. Biomed. Pharmacol. J. 2017, 10, 1267–1277.
8. Bhattacharjee, S.; Waqar, A.; Barua, K.; Das, A.; Bhowmik, S.; Debi, S.R. Phytochemical and pharmacological evaluation of Methanolic Extract of Lathyrus Sativus L. Seeds. Clin. Phytoscience 2018, 4, 1–9.
9. Hasan, M.M.; Hossain, A.; Shamim, A.; Rahman, M.M. Phytochemical and pharmacological evaluation of Ethanolic Extract of Lepisanthes Rubiginosa L. leaves. BMC Complement. Altern. Med. 2017, 17, 496.
10. Rosenthal, J.P. Plants, People and Culture: the Science of Ethnobotany. In Journal of Natural Products; American Chemical Society (ACS), 1997; Vol. 60, pp. 428–429.
11. Adjatin, A.; Dansi, A.; Yêvidé, A.; A.A.- R.J. of; 2013, Undefined of Gbolo (Crassocephalum crepidioides (benth.) S. Moore and C. Rubens (Juss. Jacq.) S. Moore), an Aromatic Herb Consumed as Leafy Vegetable in Benin. Cabdirect.Org 2013.
12. Arawande, J.O.; Komolafe, E.A.; Imokhuede, B. Nutritional and Phytochemical Compositions of Fireweed (Crassocephalum crepidioides). J. Agric. Technol. 2013, 9, 439–449.
13. Akah, P.A. Antidiarrheal Activity of Kigelia Africana in Experimental Animals. J. Herbs, Spices Med. Plants 1996, 4, 31–38.
14. Güllüce, M.; Adigüzel, A.; Öǧütçü, H.; Şengül, M.; Karaman, I.; Şahin, F. Antimicrobial Effects of Quercus ilex L. Extract. Phyther. Res. 2004, 18, 208–211.
15. Degu, A.; Engidawork, E.; Shibeshi, W. Evaluation of the anti-Diarrheal Activity of the leaf extract of Croton Macrostachyus Hocsht. ex Del. (Euphorbiaceae) in Mice Model. BMC Complement. Altern. Med. 2016, 16.
16. Viana, G.S.B.; Bandeira, M.A.M.; Matos, F.J.A. Analgesic and anti-inflammatory effects of chalcones isolated from Myracrodruon urundeuva Allemão. Phytomedicine 2003, 10, 189–195.
17. Akakpo, A.D.M.; Achigan-Dako, E.G. Nutraceutical Uses of Traditional Leafy Vegetables and Transmission of Local Knowledge from Parents to Children in Southern Benin. Agronomy 2019, 9, 805.
18. Kongsaeree, P.; Prabpai, S.; Sriubolmas, N.; Vongvein, C.; Wiyakrutta, S. Antimalarial dihydroisocoumarins Produced by Geotrichum sp., an Endophytic Fungus of Crassocephalum Crepidioides. J. Nat. Prod. 2003, 66, 709–711.
19. Yen, G.C.; Chen, H.Y.; Peng, H.H. Evaluation of the Cytotoxicity, Mutagenicity and Antimutagenicity of Emerging Edible plants. Food Chem. Toxicol. 2001, 39, 1045–53.
20. Mahdi-Pour, B.; Jothy, S.L.; Latha, L.Y.; Chen, Y.; Sasidharan, S. Antioxidant activity of methanol extracts of different parts of Lantana camara. Asian Pac. J. Trop. Biomed. 2012, 2, 960–965.
21. Uddin, M.S.; Hossain, M.S.; Kabir, M.T.; Rahman, I.; Tewari, D.; Jamiruddin, M.R.; Mamun, A. Al Phytochemical Screening and Antioxidant Profile of Syngonium podophyllum Schott Stems: A Fecund Phytopharmakon. J. Pharm. Nutr. Sci. 2018, 8, 120–128.
22. Aniya, Y.; Koyama, T.; Miyagi, C.; Miyahira, M.; Inomata, C.; Kinoshita, S.; Ichiba, T. Free Radical Scavenging and Hepatoprotective Actions of the Medicinal Herb, Crassocephalum crepidioides from the Okinawa Islands. Biol. Pharm. Bull. 2005, 28, 19–23.
23. L, L.Y. Phytochemical screening and toxicity studies of Crassocephalum rubens (Juss. ex Jacq.) S. Moore and Crassocephalum crepidioides (Benth.) S. Moore consumed as Vegetable in Benin. Available online www.jocpr.com J. Chem. Pharm. Res. 2013, 160–167.
24. Aniya, Y.; Koyama, T.; Miyagi, C.; Miyahira, M.; Inomata, C.; Kinoshita, S.; Ichiba, T. Free Radical Scavenging and Hepatoprotective actions of the Medicinal Herb, Crossocephalum Crepidioides from the Okinawa Islands. Biol. Pharm. Bull. 2005, 28, 19–23.
25. Hossain, M.I.; Mahmood, A. Al Study on in-vitro Thrombolytic activity of methanolic extract of Mesua ferrea leaves. 2015, 52–55.
26. Ayodele, O.O.; Onajobi, F.D.; Osoniyi, R.O. In vitro anticoagulant effect of Crassocephalum crepidioides leaf methanol extract and fractions on human blood. J. Exp. Pharmacol. 2019, Volume 11, 99–107.
27. Vardin, H.; Fenercioǧlu, H.; Ruban, A. V.; Horton, P.; Young, A.J.; Hoepffner, N.; Sathyendranath, S.; Standard, T.; Stan, C.; Stan, C.; et al. Folin Ciocalteau Phenolic Content Quantification Assay Kit 400 tests (96 well plate). Innov. Food Sci. Emerg. Technol. 2005, 9, 272–279.
28. Blainski, A.; Lopes, G.C.; De Mello, J.C.P. Application and analysis of the Folin Ciocalteu Method for the Determination of the total Phenolic content from Limonium Brasiliense L. Molecules 2013, 18, 6852–6865.
29. Kedare, S.B.; Singh, R.P. Genesis and development of DPPH method of antioxidant assay. J. Food Sci. Technol. 2011, 48, 412–422.
30. Ramjan, A.; Hossain, M.; Runa, J.F.; Md, H.; Mahmodul, I. Evaluation of Thrombolytic Potential of three Medicinal Plants available in Bangladesh, as a Potent source of Thrombolytic Compounds. Avicenna J. Phytomedicine 2014, 4, 430–6.
31. Sarah, Q.S.; Anny, F.C.; Misbahuddin, M. Brine shrimp Lethality assay. Bangladesh J. Pharmacol. 2017, 12, 186–189.
32. Pimentel Montanher, A.B.; Pizzolatti, M.G.; Costa Brighente, I.M. An application of the Brine Shrimp Bioassay for general Screening of Brazilian Medicinal plants. Acta Farm. Bonaer. 2002, 21, 175–178.
33. Brine shrimp lethality assay of Sesbania grandiflora ethanolic extract – in vitro study. J. Adv. Pharm. Educ. Res. 2023, 7.
34. Awang-kanak, F.; Fadzelly, M.; Bakar, A. Ethnobotanical note, total Phenolic content, total Flavonoid content, and antioxidative activities of Wild edible Vegetable, Crassocephalum crepidioides from Kota Belud, Sabah Ethnobotanical note, total Phenolic content, total flavonoid content, a. 2019.
35. R. Hamidi, M.; Jovanova, B.; Kadifkova Panovska, T. Toxicological Evaluation of the plant products using Brine Shrimp (Artemia salina L.) model. Maced. Pharm. Bull. 2014, 60, 9–18.

Recomonded Articles:

Author(s): U.S Mahadeva Rao, Khamsah Suryati Mohd, Abdurrazaq Muhammad, Bashir Ado Ahmad, Mohaslinda Mohamad, Rosmawati Mat Ali

DOI: Not Available         Access: Open Access Read More

Author(s): Deepak Karki, Gururaj S. Kulkarni, Shivakumar Swamy, Sheeba FR

DOI: 10.5958/0974-360X.2017.00750.8         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Hayat M. Mukhtar, Vandna Kalsi

DOI: 10.5958/0974-360X.2018.00395.5         Access: Open Access Read More

Author(s): Som K. Madhvi, Manik Sharma, Javaid Iqbal, Mohd Younis

DOI: 10.5958/0974-360X.2019.00785.6         Access: Open Access Read More

Author(s): Praveen D, Ranadheer Chowdary P, Gandikota Thanmayi, Gangabathina Poojitha, Vijey Aanandhi M

DOI: 0.5958/0974-360X.2016.00047.0         Access: Open Access Read More

Author(s): Mythili. L, GNK. Ganesh, C. Monisha, Kayalvizhi. R

DOI: 10.5958/0974-360X.2019.00426.8         Access: Open Access Read More

Author(s): Siripurapu Sriram, Venkata Ravibabu Mandla, Chaithanya Sudha M, Saladi S.V. Subba Rao, Nagaveni Ch, Vani V., Gobinath P., Raj Kumar KVG., Jaydeep Lella, Sannidhi Krishna Praveen, Debjit Datta

DOI: 10.5958/0974-360X.2017.00078.6         Access: Open Access Read More

Author(s): Fathima Mariyam Niyas

DOI: 10.5958/0974-360X.2015.00182.1         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): Archana Kushwaha, Jayanti Jaiswal, Priya Singh, Neha Rathore, Jai Prakash Dhruw, Ishu Sahu, Chhaya Singh, Krinsha Kumar Sahu, Mukta Agrawal, D. K. Tripathi, Ajazuddin, Amit Alexander

DOI: 10.5958/0974-360X.2017.00270.0         Access: Open Access Read More

Author(s): Gopinath G, Thirumal M, P. R. Kumar

DOI: 10.5958/0974-360X.2020.00362.5         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags