Author(s): Mariia Shanaida, Izabela Jasicka-Misiak, Marietta Bialon, Olha Korablova, Piotr P. Wieczorek

Email(s): shanayda-mi@ukr.net

DOI: 10.52711/0974-360X.2021.00385   

Address: Mariia Shanaida1*, Izabela Jasicka-Misiak2, Marietta Bialon2, Olha Korablova3, Piotr P. Wieczorek2
1Department of Pharmacognosy and Medical Botany, I. Horbachevsky Ternopil National Medical University, Voli 1, Ternopil, Ukraine.
2Faculty of Chemistry, University of Opole, Kopernika 11, Opole, Poland.
3M.M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine, Kyiv, Ukraine.
*Corresponding Author

Published In:   Volume - 14,      Issue - 4,     Year - 2021


ABSTRACT:
Two different methods of chromatographic analysis have been used in this study for the phytochemical evaluation of main secondary metabolites in the aerial part of bee balm (Monarda fistulosa L.) as the non-officinal medicinal plant of the Lamiaceae Martinov family. The high performance thin layer chromatography (HPTLC) fingerprinting method was developed for the qualitative analyses of phenolic and non-polar compounds in the bee balm herb after its maceration in the solvents of different polarity. Such polyphenols as rosmarinic, caffeic and chlorogenic acids were authentically identified in the methanol extract of herb using HPTLC. Aromatic monoterpenoid thymol was identified by the HPTLC method in the extracts obtained with non-polar solvents (toluene, methylene chloride, and chloroform). 38 volatile compounds were determined in the methylene chloride extract of M. fistulosa herb by gas chromatography mass spectrometry (GC/MS); it was taken into account only components with the content more than 0.2 %. The GC/MS analysis showed that thymol (23.73 %), followed by carvacrol (10.09 %), p-cymene (9.74 %), and thymoquinone (8.52 %) were the major constituent of methylene chloride extract. Used chromatographic techniques may be recommended for the reliable phytochemical authentication of the M. fistulosa herb.


Cite this article:
Mariia Shanaida, Izabela Jasicka-Misiak, Marietta Bialon, Olha Korablova, Piotr P. Wieczorek. Chromatographic Profiles of the main Secondary Metabolites in the Monarda fistulosa L. Aerial Part. Research Journal of Pharmacy and Technology. 2021; 14(4):2179-4. doi: 10.52711/0974-360X.2021.00385

Cite(Electronic):
Mariia Shanaida, Izabela Jasicka-Misiak, Marietta Bialon, Olha Korablova, Piotr P. Wieczorek. Chromatographic Profiles of the main Secondary Metabolites in the Monarda fistulosa L. Aerial Part. Research Journal of Pharmacy and Technology. 2021; 14(4):2179-4. doi: 10.52711/0974-360X.2021.00385   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-4-61


REFERENCES: 
1. Bourgaud F, Gravot A, Milesi S, Gontier E. Production of plant secondary metabolites: a historical perspective. Plant Science. 2001;161 (5): 839-851.
2. Hussein RA, El-Anssary AA. Plants secondary metabolites: the key drivers of the pharmacological actions of medicinal plants. Herbal medicine. 2018. 21 p.
3. Wolfender J-L, Kubo MD, Queiroz EF, and Allard PM. Contextualized metabolomics transforms pharmacognosy – a paradigm shifts in natural product research. The 66th Annual meeting of the Society for Medicinal and Natural Product Research GA-2018 (Shanghai, Chine, August 26-29, 2018). 
4. Raksha Mukhia, Bharat Basistha, Dhani Raj Chhetri. Variation in antioxidant activity of a rattan species, Plectocomia himalayana Griff. by DPPH assay based on two different methods of methanol extraction. Res. J. Pharmacognosy and Phytochem. 2018; 10(2): 175-178.
5. Sampathkumar Y, Mahadevan SG, Jayaraman R. Physicochemical, Phytochemical screening and HPTLC Fingerprinting Analysis of Ethanolic extract of Mimusops elengi Linn. leaves. Research J. Pharm. and Tech 2020; 13(5): 2091-2095. 
6. Gontova TM, Sokolova OO, Kotov AG, Kutsenko SA, Mashtaler VV. Determination of essential oil component composition of common sunflower marginal flowers. Research J. Pharm. and Tech 2018; 11(5): 1971-1973.
7. Koshovyi O, Raal A, Kovaleva A, Myha M, Ilina T, Borodina N, Komissarenko A. The phytochemical and chemotaxonomic study of Salvia spp.  growing in Ukraine. J Appl Biol Biotech 2020;8(03): 029–036. 
8. Mattarelli P, Epifano F, Minardi P, Di Vito M, Modesto M, Barbanti L, Bellardi MG. Chemical composition and antimicrobial activity of essential oils from aerial parts of Monarda didyma and Monarda fistulosa cultivated in Italy. J. of Ess. Oil Bear. Plants. 2017; 20(1): 76–86.
9. Mazza G, Chubey BB, Kiehn F. Essential oil of Monarda fistulosa L. var. menthaefolia, a potential source of geraniol. Flavour Frag. 1987; 2: 129–132. 
10. Shanayda MI. Phytochemical research of Monarda fistulosa L. aerial part. Farmatsevtychnyi zhurnal. 2010; 5: 89-93.
11. Native plants, native healing: traditional Muskogee way / Ed. by T. M. Crow. BPC, 2001: 39–52. 
12. Shanaida M, Jasicka-Misiak I, Makowicz E, Stanek N, Shanaida V, Wieczorek PP. Development of the HPTLC method for identifications of phenolic compounds and quantification of rosmarinic acid content in some Lamiaceae Martinov species. J. Pharm. Bioall. Sci. 2020; 12: 139-145.
13. Sovova H, Sajfrtova M, Topiar M. Supercritical CO2 extraction of volatile thymoquinone from Monarda didyma and M. fistulosa herbs. J. of Supercrit. Fluids. 2015; 105: 29–34. 
14. Thompson T, Kiehne P, Maroko J, Kapsner TR, Angerhofer CK. Seasonal variation in chemistry and biological activity of Monarda fistulosa. Planta Med. 2013; 79: 11–17. 
15. The Plant List. http://www.theplantlist.org
16. Shilpa M, Kuppast IJ, Virupaksha JH, Vanajakshi M, Prasad K. Glossary of Callistemon lanceolatus D.C. – an ornamental plant with marvellous properties. Research Journal of Pharmacology and Pharmacodynamics. 2015; 7(4): 165-170.
17. European Pharmacopoeia. 9th Ed. 2016: https://www.edqm.eu/en/european-pharmacopoeia-ph-eur-9th-edition.
18. Stanek N, Jasicka-Misiak I. HPTLC phenolic profiles as useful tools for the authentication of honey. Food Analytical Methods. 2018; 11(11): 2979–2989.
19. Ashutosh P. Q-markers or chemical markers: a new insight towards quality control of herbal medicines. Organic & Med Chem I J. 2017; 3(2): 1–2.
20. Asha D, Mathew L, Kalappurakkal R. Evaluation of HPTLC fingerprints of flavonoids and antioxidant activity of selected medicinal plants of Lamiaceae family. Int. J. of Pharmacognosy and Phytochemical Res. 2015; 7(2): 240-245.
21. Benedec D, Hanganu I, Oniga I, Tiperciuc B, Olah NK, Raita O, Bischin C, Silaghi-Dumitrescu R, Vlase L. Assessment of rosmarinic acid content in six Lamiaceae species extracts and their antioxidant and antimicrobial potential. Pak. J. Pharm. Sci. 2015; 28 (6): 2297–2303.
22. Jakovljević M, Vladić J, Vidović S, Pastor K, Jokić S, Molnar M, Jerković I. Application of deep eutectic solvents for the extraction of rutin and rosmarinic acid from Satureja montana L. and evaluation of the extracts antiradical activity. Plants. 2020; 26, 9 (2): 153. doi: 10.3390/plants9020153.
23. Janicsák G, Háznagy-Radnai E, Engel R, Blunden G, Máthé I. TLC-densitometry of rosmarinic and caffeic acids in the evaluation of Lamiaceae species growing in Central Europe. Journal of Planar Chromatography - Modern TLC. 2013; 26 (2): 132–136. 
24. Jasicka-Misiak I, Poliwoda A, Petecka P, Buslovych O, Shlyapnikov V, Wieczorek PP. Antioxidant phenolic compounds in Salvia officinalis L. and Salvia sclarea L. Ecol. Chem. Eng S. 2018; 25(1): 133-142. 
25. Long W, Yuan-Yua J, Li Z, Tao W, Rui-Wu Y, Chun-Bang D, Xiao-Li W, Yong-Hong Z. High-Performance Liquid Chromatography Fingerprints and simultaneous quantification of bioactive compounds in Salvia przewalskii Maxim. Acta Chromatographica. 2017; 29 (3): 291–308.
26. Shanaida M, Golembiovska O, Hudz N, Wieczorek PP. Phenolic compounds of herbal infusions obtained from some species of the Lamiaceae family. Current Issues in Pharmacy and Medical Sciences. 2018; 31 (4): 194-199. 
27. Staszek D, Orłowska M, Waksmundzka-Hajnos M, Sajewicz M, Kowalska T. Marker fingerprints originating from TLС and HPLC for selected plants from the Lamiaceae family. J. Chromatography. 2013; 36 (17): 2463–2475.
28. Canrong W, Yang L, Yueying Y, Zhang P, Zhong W et al. Analysis of therapeutic targets for SARS-CoV-2 and discovery of potential drugs by computational methods. Acta Pharmaceutica Sinica B. 2020. Available at: https://doi.org/10.1016/j. apsb.2020.02.00
29. Huai Cao, Wei-Xian Cheng, Cong Li, Xu-Lin Pan, Xiao-Guang Xie, Tao-Hong Li. DFT study on the antioxidant activity of rosmarinic acid. Journal of Molecular Structure: THEOCHEM. 2005; 719 (1–3): 177-183.
30. Dhanya Rajan, Jisna K, Anas Hamza, Fathimathul Rishana. Comparative Review on Pharmacognostical and Pharmacological activities of Ocimum Species. Res. J. Pharmacognosy and Phytochem. 2020; 12(1): 37-46.
31. Hudz N, Yezerska O, Shanaida M, Horčinová Sedláčková V, Wieczorek PP. Application of the Folin-Ciocalteu method to the evaluation of Salvia sclarea extracts. Pharmacia. 2019; 66(4): 209-215.
32. Jaydeokar AV, Bandawane DD, Nipate SS, Chaudhari PD. Natural antioxidants: a review on therapeutic applications. Research J. Pharmacology and Pharmacodynamics. 2012; 4(1): 55-61.
33. Khomdram S, Potsangbam K. Polyphenolic сompounds and free radical scavenging activity in eight Lamiaceae herbs of Manipur. Not. Sci. Biol. 2011; 3: 108-113. 
34. Mohammed Yahya Hadi, Imad Hadi Hameed, Israa Adnan Ibraheam. Mentha pulegium: medicinal uses, anti-hepatic, antibacterial, antioxidant effect and analysis of bioactive natural compounds: a review. Research J. Pharm. and Tech 2017; 10(10): 3580-3584. 
35. Priya Dongare, Swati Dhande, Vilasrao Kadam. A Review on Pogostemon patchouli. Res. J. Pharmacognosy & Phytochem. 2013; 6(1): 41-47.
36. Rytsyk O, Soroka Y, Shepet I, Vivchar Z, Andriichuk I. et al. Experimental evaluation of the effectiveness of resveratrol as an antioxidant in colon cancer prevention. Natural Product Communications. 2020. 15(6):1934578X2093274
37. Posokhova K, Stechyshyn I, Krynytska I, Marushchak M, Birchenko I, Klishch I. Comparative study of the effect of various forms of quercetin on experimental diabetes. Rom J Diabetes Nutr Metab Dis. 2018. 25(4): 383-388.
38. Frolova N, Uktainets A, Korablova O, Voitsekhivskyi V. Plants of Nepeta cataria var. citriodora Beck. and essential oils from them for food industry. Potravinarstvo: Slovak Journal of Food Sciences. 2019; 13(1): 449-455. 
39. Gursain AI, Anwar F, Sherazi HS, Przybylski R. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chem. 2008; 108 (3): 986–995.
40. Rim M Harfouch, Manal Darwish, Wisam Al-Asadi, Ali F. Mohammad, Nour M. Gharib, Mohammad Haroun. Antibacterial activity of essential oils of Rosmarinus officinalis, Salvia officinalis and Anthemis nobilis widespread in the Syrian Coast. Research J. Pharm. and Tech. 2019; 12(7): 3410-3412.
41. Shanaida M, Pryshlyak A, Golembiovska O. Determination of triterpenoids in some Lamiaceae species. Research J. Pharm. and Tech. 2018; 7: 3113–3118. 
42. Deniz GY. The protective effects of thymol against ketoprofen induced damages on pancreatic acinar and islet of langerhans cells in rats. J. of Ess. Oil Bear. Plants. 2019; 22(3): 604-613.
43. Games E, Guerreiro M, Fernanda RS, Pinheiro NM, De Oliveira EA. Structurally related monoterpenes p-cymene, carvacrol and thymol isolated from essential oil from leaves of Lippia sidoides Cham. (Verbenaceae). Protect Mice against Elastase-Induced Emphysema. Molecules. 2016; 21(10): 1390.
44. Lee JH, Kim YG, Lee J. Carvacrol-rich oregano oil and thymol-rich thyme red oil inhibit biofilm formation and the virulence of uropathogenic Escherichia coli. J. Appl. Microbiol. 2017; 123 (6): 1420-1428.
45. Sharifi-Rad M, Varoni EM, Iriti M, Martorell M, Setzer WN, et al. Carvacrol and human health: a comprehensive review. Phytother. Res. 2018; 32 (9): 1675-1687.
46. Cobourne-Duval MK, Taka E, Mendonca P, Soliman KF. Thymoquinone increases the expression of neuroprotective proteins while decreasing the expression of pro-inflammatory cytokines and the gene expression NFκB pathway signaling targets in LPS/IFNγ-activated BV-2 microglia cells. J. Neuroimmunol. 2018; 15 (320): 87-97. 
47. Bule M, Nikfard S, Amini M, Abdollahi M. The antidiabetic effect of thymoquinone: A systematic review and meta-analysis of animal studies. Food Research International. 2020; 127: 108736.
48. Khan Md. A., Tania M., Fu S., Fu J. Thymoquinone, as an anticancer molecule: from basic research to clinical investigation. Oncotarget. 2017. 1; 8(31): 51907-51919.
49. Crocoll C. Biosynthesis of the phenolic monoterpenes, thymol and carvacrol, by terpene synthases and cytochrome P450s in oregano and thyme. Dissertation. Max-Planck-Institut für chemische Ökologie, 2010. 143 p.
50. Pandey AK, Nijendra PS, Tripathi N. Chemistry and bioactivities of essential oils of some Ocimum species: an overview. Asian Pacific Journal of Tropical Biomedicine. 2014; 4 (4): 682-694.
51. Shanaida M, Hudz N, Korzeniowska K, Wieczorek P. Antioxidant activity of essential oils obtained from aerial part of some Lamiaceae species. International Journal of Green Pharmacy. 2018; 12 (3): 200-204.
52. Yang Y, Zhang Z, Li S, Ye X, Li X, He K. Synergy effects of herb extracts: pharmacokinetics and pharmacodynamic basis. Fitoterapia. 2014; 92:133–147.

Recomonded Articles:

Author(s): A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani

DOI: 10.5958/0974-360X.2017.00047.6         Access: Open Access Read More

Author(s): Peethala Prathyusha, Raja Sundararajan, Palyam Bhanu, Mathrusri Annapurna Mukthinuthalapati

DOI: 10.5958/0974-360X.2020.00507.7         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): R. P. Bhole, S. R. Jagtap, K. B. Chadar, Y. B. Zambare

DOI: 10.5958/0974-360X.2020.00189.4         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj Charde, Rajesh Shukla, Rita M. Charde

DOI: Not Available         Access: Open Access Read More

Author(s): Mukta D. Naykode, Durgacharan A. Bhagwat, Swapnil D. Jadhav, Harinath N. More

DOI: 10.5958/0974-360X.2017.00133.0         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): Rituraj Singh Chundawat, Y.S. Sarangdevot, R.P.S. Rathore, Dharmendra Singh Sisodiya, Udaibhan Singh Rathore

DOI:         Access: Open Access Read More

Author(s): Anket Sharma, Vinod Kumar, Parminder Kaur, Ashwani Kumar Thukral, Renu Bhardwaj

DOI: 10.5958/0974-360X.2015.00299.1         Access: Open Access Read More

Author(s): Manisha N. Trivedi, Archana Khemani, Urmila D. Vachhani, Charmi P. Shah, D.D. Santani

DOI: Not Available         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags