Author(s): Amir Alhaj Sakur, Firas Mannaa, Mahamed Yahia Zein Eddin

Email(s): profsakur@gmail.com

DOI: 10.52711/0974-360X.2021.00375   

Address: Amir Alhaj Sakur1*, Firas Mannaa1, Mahamed Yahia Zein Eddin2
1Department of Analytical and Food Chemistry, Faculty of Pharmacy, University of Aleppo, Syria.
2Department of Chemistry, Faculty of Sciences, University of Aleppo, Syria.
*Corresponding Author

Published In:   Volume - 14,      Issue - 4,     Year - 2021


ABSTRACT:
A new chromatographic support was prepared from Local Syrian Clay (Bentonite), using thermal and acid treated Clay (B500AW) for utilizing it in thin layer chromatography (0.25mm thickness) to separate and determine of Paracetamol, Caffeine, and Aspirin in raw material and in tablets. The separation carried out using mobile phase consisted of Cyclohexane-Chloroform- Methanol - acetic acid (14:5: 0.25:0.75) v/v. The specific surface area of treated bentonite was 45m2/g. Quantification was carried out densitometerically at ? = 250nm for Paracetamol, ? = 275nm for Caffeine, and at ? = 200 nm for Aspirin. The retardation factors (Rf) of Paracetamol, Caffeine, and Aspirin were 0.10, 0.21, and 0.40 respectively. Calibration curves were obtained in the concentration ranges of 5.0-40.0µg/spot, 2.0-16.0µg/spot and 3.0-24.0µg/spot for standard solutions of Paracetamol, Caffeine and Aspirin respectively. The New Prepared Chromatographic Thin Layers were successfully applied for analysis of commercial dosage forms (tablets) containing the drugs with average recovery 98.33 –101.83% with RSD not more than 3.86%.


Cite this article:
Amir Alhaj Sakur, Firas Mannaa, Mahamed Yahia Zein Eddin. Preparing a New Chromatographic support from Local Syrian Clay and using it in Thin Layer Chromatography for Simultaneous Determination of Paracetamol, Caffeine and Aspirin in Tablets. Research Journal of Pharmacy and Technology. 2021; 14(4):2119-4. doi: 10.52711/0974-360X.2021.00375

Cite(Electronic):
Amir Alhaj Sakur, Firas Mannaa, Mahamed Yahia Zein Eddin. Preparing a New Chromatographic support from Local Syrian Clay and using it in Thin Layer Chromatography for Simultaneous Determination of Paracetamol, Caffeine and Aspirin in Tablets. Research Journal of Pharmacy and Technology. 2021; 14(4):2119-4. doi: 10.52711/0974-360X.2021.00375   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-4-51


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