Author(s): Yamin, Ruslin, Mistriyani, La Ode Muhammad Fitrawan, Sabarudin, Muhammad Arba

Email(s): muh.arba@uho.ac.id

DOI: 10.52711/0974-360X.2021.00363   

Address: Yamin, Ruslin, Mistriyani, La Ode Muhammad Fitrawan, Sabarudin, Muhammad Arba
Faculty of Pharmacy, Universitas Halu Oleo, Kampus Bumi Tridharma Anduonohu, Kendari - South East Sulawesi, Indonesia.
*Corresponding Author

Published In:   Volume - 14,      Issue - 4,     Year - 2021


ABSTRACT:
Introduction: Okra, which belong to Malvaceae family, is well known used as a food ingredient due to its high fiber content. Objective: In this study, the antiradical strength of okra seed extracts and fractions was investigated. Methodology: Okra seeds were dried and macerated with ethanol. Further, it is fractionated by different solvents based on the level of polarity from the non-polar to the polar, including n-hexane, chloroform, ethyl acetate and water. The extracts and fractions were then tested for antiradical strength using DPPH and analysis of phenolic and flavonoid contents were performed. Results: Ethanol extract and fractions of okra seeds showed very strong free antiradical activity, and ethyl acetate fraction being strongest fraction with IC50 value of 10.097±0.074µg/mL. Meanwhile, the phenolic and flavonoid contents of ethyl acetate fraction were the highest among other fractions with the values of 61.412±0.196 EAG and 97.933±0.222 EQ, respectively, with correlation values (R2) 0.9281 (phenolic) and 0.6229 (Flavonoids). Conclusion: Extract and ethyl acetate fraction of Okra seed had good radical inhibition properties, which indicated its potential for further development assays.


Cite this article:
Yamin, Ruslin, Mistriyani, La Ode Muhammad Fitrawan, Sabarudin, Muhammad Arba. Free Radical Scavenging Activity of extract and fraction of Okra seeds determined by DPPH (2,2-diphenyl-1-picrylhydrazy) method. Research Journal of Pharmacy and Technology. 2021; 14(4):2045-8. doi: 10.52711/0974-360X.2021.00363

Cite(Electronic):
Yamin, Ruslin, Mistriyani, La Ode Muhammad Fitrawan, Sabarudin, Muhammad Arba. Free Radical Scavenging Activity of extract and fraction of Okra seeds determined by DPPH (2,2-diphenyl-1-picrylhydrazy) method. Research Journal of Pharmacy and Technology. 2021; 14(4):2045-8. doi: 10.52711/0974-360X.2021.00363   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-4-39


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