Author(s): Neelamma G, Shaik Harun Rasheed, Anjaneyulu Vinukonda, Aruna M

Email(s): nanisony2012@gmail.com

DOI: 10.52711/0974-360X.2021.00356   

Address: Neelamma G1*, Shaik Harun Rasheed2, Anjaneyulu Vinukonda3, Aruna M2
1Department of Pharmacognosy, Srikrupa Institute of Pharmaceutical Sciences, Velikatta, Kondapak, Siddipet, Telangana, India.
2Department of Pharmaceutics, Srikrupa Institute of Pharmaceutical Sciences, Velikatta, Kondapak, Siddipet, Telangana, India.
3Team Lead, MS & T, Technical Operations, Alembic Pharmaceuticals, Vadodara, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 4,     Year - 2021


ABSTRACT:
Clitoria ternatea, Cucurbita maxima, Artemisia vulgaris were the Indian native plants used in the treatment of different diseases. The methanolic extract of Clitoria ternatea, petroleum ether extract of Cucurbita maxima and methanolic extract of Artemisia vulgaris were investigated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. These extracts were assessed using N2a cell line (Neuroblastoma). Cisplatin was used as a positive control based on IC50 values. Artemisia vulgaris methanol extract have highest cytotoxicity (80.81ug/mL) and Cucurbita maxima seed extract have lowest cytotoxicity (57.655ug/mL). The comparison of IC50 of the extracts had shown the following toxicity: Cucurbita maxima petroleum ether extract


Cite this article:
Neelamma G, Shaik Harun Rasheed, Anjaneyulu Vinukonda, Aruna M. In-vitro Screening for Cytotoxic Activity of Herbal Plant Extracts on N2a Cell Lines. Research Journal of Pharmacy and Technology. 2021; 14(4):2009-2. doi: 10.52711/0974-360X.2021.00356

Cite(Electronic):
Neelamma G, Shaik Harun Rasheed, Anjaneyulu Vinukonda, Aruna M. In-vitro Screening for Cytotoxic Activity of Herbal Plant Extracts on N2a Cell Lines. Research Journal of Pharmacy and Technology. 2021; 14(4):2009-2. doi: 10.52711/0974-360X.2021.00356   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-4-32


REFERENCES:
1. Rangarajan Narasimhan, Sathiyamoorthy. Phytochemical Screening and Antioxidant Studies in the Pulp Extracts of Cucurbita maxima. Asian J. Pharm.Res.2016; 6(1): 1-4.
2. Mukesh Kumar Singh, Prathapan A, Kushagra Nagori S, Ishwarya and Raghu K. Cytotoxic and Antimicrobial Activity of Methanolic Extract of Boerhaavia diffusa L. Research J. Pharm. and Tech. 2010; 3(4).
3. Pappachen K, Sreelakshmi KS. Phytochemical Screening and Invitro Cytotoxicity Studies of Mussaenda frondosa Linn Leaves Leena. Research J. Pharm. and Tech. 201; 10(12): 422-4330.
4. Kadiri Sunil Kumar, Avanapu Srinivasa Rao. Evaluation of hepato protective and in-vitro cytotoxic activity of leaves of Chrozophoraplicata Linn, Research Journal of Pharmacology and Pharmacodynamics. 2017; 9(1): 19-26.
5. Rangarajan Narasimhan, Sathiyamoorthy M. Phytochemical Screening and Antioxidant Studies in the Pulp Extracts of Cucurbitamaxima. Asian J. Pharm. Res. 2016; 6(1): 1-4.
6. Durga Devi, Banu N. Anti-Proliferative activity of Chlorophyllin from Phyllanthus emblica L against MCF-7 and Vero Cell line. Research J. Pharm. and Tech. 2017; 10(2): 516-524.
7. Saroj Kumar Raul, Gopal Krishna Padhy, Soudamini Alekha Charan, Neelima Gurubarik. Comparative Evaluation of Anthelmintic Potential of Cucurbita maxima Seeds, Moringa oleifera Seeds and Solanum surattense Fruits, Research Journal of Pharmacognosy and Phytochemistry. 2014; 6(4): 187-189.
8. Anjali Soni, Patel Femida, Preeti Sharma. In-vitro Cytotoxic Activity of Plant Saponin Extracts on Breast Cancer Cell-Line. Research Journal of Pharmacognosy and Phytochemistry. 2017; 9(1): 17-22.
9. Kangralkar VA and Kulkarni AR. In Vitro Cytotoxic Activity of Alcoholic Extract of Aristolochia indica. Research J. Pharm. and Tech. 2013; 6(11): 1240-1244.
10. Manokaran S, Saravanan V.S, Kulanthavel T.M. and Kumarrapan CT. Haematological activity of Cucurbita maxima Linn. Pulp In Phenylhydrazine Induced Anaemic Rats. Research J. Pharmacognosy and Phytochemistry. 2010; 2(5): 395-396.
11. Elisa Vega-Avila, Adolfo Espejo-Serna, Francisco Alarcón-Aguilar and Rodolfo Velasco-Lezama. Proc. West. Pharmacol. Soc. 2009; 52: 78-82.
12. Betts DR, Cohen N, Leibundgut KE, Kuhne T, Caflisch U and Greiner J. Characterization of karyotypic events and evolution in neuroblastoma. Pediatric Blood and Cancer. 2005;44: 147–157.
13. Park JR, Eggert A and Caron H. Neuroblastoma: biology, prognosis, and treatment. Pediatric Clinics of North America. 2008; 55: 97–120.
14. Navarra M, Celano M, Maiuolo J, Schenone S, Botta M and Angelucci A. Antiproliferative and pro-apoptotic effects afforded by novel SRC-kinase inhibitors in human neuroblastoma cells.  BMC Cancer. 2010; 10: 602.
15. KangJ, Kamal A, Burrows FJ, Evers BM and Chung DH. Inhibition of Neuroblastoma. Anticancer Res. 2006; 26(3A): 1903–1908.
16. Svensson L, Lilliehook B, Larsson R and Bucht A. Gammadelta T cells contribute to the systemic immunoglobulin E response and local B-cell reactivity in allergic eosinophilic airway inflammation. Immunology. 2003; 108(1): 98-108.
17. Sithranga Boopathy N, Kathiresan K. Anticancer Drugs from Marine Flora: An overview journal of oncology. 2010; 1-18.
18. Kilan Jaziri Soumaya, Ghedira Zied, Nasr Nouha, Krifa Mounira, Ghedira Kamel, Franca Dijoux Marie Genvieve, Ghedira Chekir Leila. Evaluation of invitro antioxidant and apoptotic activities of Cyperusrotundus. Asian Pacific Journal of Tropical Medicine. 2014; 7(2): 105.
19. Sghaiera MB, Bhouria W, Bouhlela I, Skandrania I, Boubakera J, Chekir- Ghediraa, Ghedira K. Inhibitory effect of Teucrium ramosissimum extracts on aflatoxin B1,benzopyrene, 4-nitro-o-phenylenediamine and sodiumazide induced mutagenicity: Correlation with antioxidant activity. South African Journal of Botany. 2011; 77: 730-740.
20. Lawson-Evi Povi1, Diallo Aboudoulatif, Bakoma Batomayena, Moukha Serge, Eklu-Gadegbeku Kwashie1, Aklikokou Kodjo1, Creppy Edmond and Gbéassor Mensanvi1. Cytotoxicity of plant extract on Neuro-2a, Caco-2 and Vero cell lines by using MTT assay and Neutral Red uptake test. Annals of Pharma Research. 2015; 3(07): 129-131.
21. Diaz P, Jeong SC, Lee S, Koo C and Koyyalamudi SR. Antioxidant and anti-inflammatory activities of selected medicinal plants and fungi containing phenolic and flavonoid compounds. Chinese Medicine. 2012; 7(26): 1-9.

Recomonded Articles:

Author(s): Koushika Das, Pranit Krishna, Avipsha Sarkar, Shanmuga Sundari Ilangovan, Shampa Sen

DOI: 10.5958/0974-360X.2017.00267.0         Access: Open Access Read More

Author(s): Karpagam T, Jannathul Firdous, Revathy, Shanmuga Priya, Varalakshmi B, Gomathi S, Geetha S, Noorzaid Muhamad

DOI: 10.5958/0974-360X.2019.00360.3         Access: Open Access Read More

Author(s): Mukesh Kumar Singh, A. Prathapan, Kushagra Nagori, S. Ishwarya, K.G Raghu

DOI:         Access: Open Access Read More

Author(s): Sumit Phulera, Neelam Gurung, Kavita Maria Arora, Gaurav Kumar, Loganathan Karthik, Kokati Venkata Bhaskara Rao

DOI: Not Available         Access: Open Access Read More

Author(s): Amaq Fadholly, Arif N. M. Ansori, Shara Jayanti, Annise Proboningrat, Muhammad K. J. Kusala, Naimah Putri, Fedik A. Rantam, Sri A. Sudjarwo

DOI: 10.5958/0974-360X.2019.00591.2         Access: Open Access Read More

Author(s): Merin Babu, Subin Varghese Thomas, Sruthi T P, Jipnomon Joseph

DOI: 10.5958/0974-360X.2019.00651.6         Access: Open Access Read More

Author(s): NasimunIslam N1., Jemimah Naine S., Shaik Jameel, S.S. Khora, Venkat Kumar, Mohanasrinivasan V., C. Subathra Devi, Panneerselvam A.

DOI: Not Available         Access: Open Access Read More

Author(s): Sarika R. Deshmukh, Solomon Habtemariam, Prasad A. Wadegaonkar

DOI:         Access: Open Access Read More

Author(s): Rathi M.A., Meenakshi P., Guru Kumar D., Arul Raj C., Sunitha M., Gopalakrishnan V.K.

DOI: Not Available         Access: Open Access Read More

Author(s): Suko Hardjono, Tri Widiandani, Bambang T. Purwanto, Anindi L. Nasyanka

DOI: 10.5958/0974-360X.2019.00359.7         Access: Open Access Read More

Author(s): Ravula Nanditha, Saravanan J, Praveen T K, Deepa S, Emdormi Rymbai

DOI: 10.5958/0974-360X.2020.00733.7         Access: Open Access Read More

Author(s): Abhishek Jain, Varsha Ojha, Gaurav Kumar, Loganathan Karthik, Kokati Venkata Bhaskara Rao

DOI: Not Available         Access: Open Access Read More

Author(s): Karthiga Muralidharan, Punnagai Kumaravelu, Darling Chellathai David

DOI: 10.5958/0974-360X.2020.00165.1         Access: Open Access Read More

Author(s): Arti Majumdar, Nidhi Dubey, Nitin Dubey

DOI: 10.5958/0974-360X.2020.00270.X         Access: Open Access Read More

Author(s): Ratu Shafira Hanifah, Nadia Aida Novitarani, Fadhilah Harmen, Aryo Tedjo, Norma Nur Azizah, Rista Putrianingsih, Wilzar Fachri, Kusmardi Kusmardi

DOI: 10.5958/0974-360X.2019.00489.X         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags