Author(s): Ira Humairah, Lina Lukitasari, Retno Handajani, Mochamad Amin, Citrawati Dyah Kencono Wungu, Edhi Rianto, Arif Nur Muhammad Ansori

Email(s): humairah.ira@gmail.com

DOI: 10.52711/0974-360X.2021.00354   

Address: Ira Humairah1*, Lina Lukitasari1, Retno Handajani1,2, Mochamad Amin1,2, Citrawati Dyah Kencono Wungu1, Edhi Rianto1, Arif Nur Muhammad Ansori3,4
1Department of Physiology and Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
2Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia.
3Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
4Professor, Nidom Foundation, Surabaya, Indonesia.
*Corresponding Author

Published In:   Volume - 14,      Issue - 4,     Year - 2021


ABSTRACT:
Every year, there is an increase in the number Intravenous Drug Users (IDUs) in Indonesia, in which they have a greater risk of getting Hepatitis C Virus (HCV) infection. This study was aimed to determine HCV prevalence and genotypes among IDUs in Surabaya. A total 25 IDUs were tested for anti-HCV. Reverse-transcription PCR in the NS5B region and 5'UTR was carried out on serum with positive anti-HCV, continued with sequencing and HCV genotype/subtype determination. The prevalence of HCV infection in IDUs in Surabaya based on positive anti-HCV was 96%(24/25). Sequencing analysis showed that 92%(22/24) could be determined their genotypes and subtypes. 40.9% (9/22) samples had genotype 1 with subtype 1a 31.82%(7/22) and subtype 1c 9.09%(2/22). 40.9%(9/22) samples had genotype 3, with subtype 3a 18.18%(4/22) and 3k 22.73%(5/22). 13.64%(4/22) samples had genotype 4 with subtype 4a, and 4.55% (1/22) sample had genotype 6 subtype 6n. The prevalence of HCV infection was high among IDUs in Surabaya. In this study, genotypes 6 was found in which it is rarely found in Indonesia, so there is a possibility that there are different HCV genotype patterns in each region of Indonesia.


Cite this article:
Ira Humairah, Lina Lukitasari, Retno Handajani, Mochamad Amin, Citrawati Dyah Kencono Wungu, Edhi Rianto, Arif Nur Muhammad Ansori. Prevalence and Genotype Distribution of Hepatitis C Virus among Intravenous Drug users (IDUs) in Surabaya, Indonesia. Research Journal of Pharmacy and Technology. 2021; 14(4):1999-3. doi: 10.52711/0974-360X.2021.00354

Cite(Electronic):
Ira Humairah, Lina Lukitasari, Retno Handajani, Mochamad Amin, Citrawati Dyah Kencono Wungu, Edhi Rianto, Arif Nur Muhammad Ansori. Prevalence and Genotype Distribution of Hepatitis C Virus among Intravenous Drug users (IDUs) in Surabaya, Indonesia. Research Journal of Pharmacy and Technology. 2021; 14(4):1999-3. doi: 10.52711/0974-360X.2021.00354   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-4-30


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