Author(s): Lokesh Ravi, Krishnan Kannabiran

Email(s): kkb@vit.ac.in

DOI: 10.52711/0974-360X.2021.00326   

Address: Lokesh Ravi1, Krishnan Kannabiran2*
1Department of Botany, St. Joseph’s College (Autonomous), Bengaluru - 27.
2Department of Biomedical Sciences, School of Biosciences and Technology, Vellore Institute of Technology, Vellore-14.
*Corresponding Author

Published In:   Volume - 14,      Issue - 4,     Year - 2021


ABSTRACT:
Aqueous extract of bark of Kigelia pinnata was used as reducing source for the biological green synthesis of AgNPs. The synthesized particles were characterized by UV-Visible spectrum, XRD, SEM, dynamic light scattering (DLS). The anti-fungal activity of AgNPs was evaluated against Aspergillus niger (MTCC:281), Aspergillus flavus (MTCC:277) and Candida albicans (MTCC:227) by agar diffusion method. Particle size of AgNPs was measured as 76.4nm ± 6.3nm and Zeta potential was stable at -43.2mV using DLS analysis. SEM analysis measured the size of AgNPs as ~75nm. XRD analysis confirmed that the synthesized NPs were silver (Ag), based on the JCPDS entry 85-1355 and it is crystalline in nature. The size of the crystal was calculated as 10nm using the Scherrer formula. The AgNPs demonstrated significant anti-fungal activity against C. albicans (MIC:15.6µg/ml), A. niger (MIC:62.4µg/ml) and A. flavus (MIC:31.2µg/ml). These results confirm that AgNPs synthesized using K. pinnata barks could be used as potential antifungal agent against fungal pathogens.


Cite this article:
Lokesh Ravi, Krishnan Kannabiran. Antifungal potential of green synthesized silver nanoparticles (AgNPS) from the stem bark extract of Kigelia pinnata. Research Journal of Pharmacy and Technology. 2021; 14(4):1842-6. doi: 10.52711/0974-360X.2021.00326

Cite(Electronic):
Lokesh Ravi, Krishnan Kannabiran. Antifungal potential of green synthesized silver nanoparticles (AgNPS) from the stem bark extract of Kigelia pinnata. Research Journal of Pharmacy and Technology. 2021; 14(4):1842-6. doi: 10.52711/0974-360X.2021.00326   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-4-2


REFERENCES:
1.    Jackson S, Beckett K. Sausage Tree Kigelia pinnata: An Ethnobotanical and Scientific Review. Am Bot Counc. 2012;(94): 48-59.
2.    Kumar S, Kumar V, Prakash OM. Antidiabetic and hypolipidemic activities of Kigelia pinnata flowers extract in streptozotocin induced diabetic rats. Asian Pac J Trop Biomed. 2012;2(7):543-546. doi:10.1016/S2221-1691(12)60093-8
3.    Namita P, Mukesh R, Tirath K. Evaluation of anti-inflammatory potential of Kigelia pinnata leaf extract in wistar rats. Asian J Pharm Clin Res. 2012;5(1): 95-97.
4.    Ahmed S, Ahmad M, Swami BL, Ikram S. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise. J Adv Res. 2016. doi:10.1016/j.jare.2015.02.007
5.    Sharma VK, Yngard RA, Lin Y. Silver nanoparticles: Green synthesis and their antimicrobial activities. Adv Colloid Interface Sci. 2009;145(1-2): 83-96. doi:10.1016/j.cis.2008.09.002
6.    Ge L, Li Q, Wang M, Ouyang J, Li X, Xing MMQ. Nanosilver particles in medical applications: Synthesis, performance, and toxicity. Int J Nanomedicine. 2014;9(1): 2399-2407. doi:10.2147/IJN.S55015
7.    Yih TC, Al-Fandi M. Engineered nanoparticles as precise drug delivery systems. J Cell Biochem. 2006;97(6):1184-1190. doi:10.1002/jcb.20796
8.    Ponarulselvam S, Panneerselvam C, Murugan K, Aarthi N, Kalimuthu K, Thangamani S. Synthesis of silver nanoparticles using leaves of Catharanthus roseus Linn. G. Don and their antiplasmodial activities. Asian Pac J Trop Biomed. 2012;2(7):574-580. doi:10.1016/S2221-1691(12)60100-2
9.    Logeswari P, Silambarasan S, Abraham J. Ecofriendly synthesis of silver nanoparticles from commercially available plant powders and their antibacterial properties. Sci Iran. 2013;20(3):1049-1054. doi:10.1016/j.scient.2013.05.016
10.    Ramya M, Sylvia Subapriya M. Green synthesis of silver nanoparticles.; 2012.
11.    Naveen PBS, Padmesh T. Common Duckweed (Lemna minor) Assisted Green Synthesis of Silver Nanoparticles as Potent Anti-Fungal Nanomaterial. Res J Pharm Technol. 2014;7(9): 955-958.
12.    Dhanaraj S, Bharathiraja P, Dhandapani R, Subbaiya R, Kathiresha AK. Biosynthesis and Characterization of Silver Nanoparticles from Aspergillus niger and its Antibacterial Activity. Res J Pharm Technol. 2018;11(12): 5282-5286.
13.    S AP, S K, R M. Biosynthesis of Silver Nanoparticles by Aspergillus niger. Asian J Res Chem. 2011;4(1): 31-34.
14.    Savithramma N, Bhumi G. Biological Synthesis of Zinc oxide Nanoparticles from C atharanthus roseus (l.) G. Don. Leaf extract and validation for antibacterial activity. 2014;6(1): 208-214.
15.    Venkatesan B, Subramanian V, Tumala A, Vellaichamy E. Rapid synthesis of biocompatible silver nanoparticles using aqueous extract of Rosa damascena petals and evaluation of their anticancer activity. Asian Pac J Trop Med. 2014. doi:10.1016/S1995-7645(14)60249-2
16.    Holder IA, Boyce ST. Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture. Burns. 1994;20(5):426-429. doi:10.1016/0305-4179(94)90035-3
17.    Reddy VB, K R, G SVB, Shenoy GG. Synthesis and Antimicrobial Studies of Some Novel Benzimidazole Derivatives. Asian J Res Chem. 2009;2(2):162-167.
18.    Singh A, Rana AC. Synthesis of Some New 5-(4-(Phenylamino) Phenyl)-1, 3, 4-Thiadiazol-2-Amine Derivatives as Potent Antifungal Agents. Asian J Res Chem. 2011;4(2): 221-226.
19.    Owolabi O, Omogbai E, Obasuyi O. Antifungal and antibacterial activities of the ethanolic and aqueous extract of Kigelia africana (Bignoniaceae) stem bark. African J Biotechnol. 2007;6(14): 1677-1680.
20.    Semwal DK, Semwal RB, Ahmad A, Kamatou GP, Viljoen AM. Inhibitory Effects of Extracts and Isolates from Kigelia africana Fruits against Pathogenic Bacteria and Yeasts. Int J Biol Vet Agric Food Eng. 2014;8(12): 1169-1174.
21.    Dhanasekaranm, Abrahamgc, Mohans. The evaluation of pharmacological potential on Kigelia pinnata DC. Int J Pharma Sci Res. 2014;5(8): 489-494.
22.    Kalainila P, Ravindran RS, Rohit R, Renganathan S. Anti-Bacterial Effect of Biosynthesized Silver Nanoparticles Using Kigelia Africana. 2015.
23.    Gopinath V, Priyadarshini S, Meera Priyadharsshini N, Pandian K, Velusamy P. Biogenic synthesis of antibacterial silver chloride nanoparticles using leaf extracts of Cissus quadrangularis Linn. Mater Lett. 2013;91: 224-227. doi:10.1016/j.matlet.2012.09.102
24.    Nezamdoost T, Bagherieh-Najjar MB, Aghdasi M. Biogenic synthesis of stable bioactive silver chloride nanoparticles using Onosma dichroantha Boiss. root extract. Mater Lett. 2014;137:225-228. doi:10.1016/j.matlet.2014.08.134
25.    Awwad AM, Salem NM, Ibrahim QM, Abdeen AO. Phytochemical fabrication and characterization of silver/silver chloride nanoparticles using Albizia julibrissin flowers extract. Adv Mater Lett. 2015;6(8): 726-730. doi:10.5185/amlett. 2015.5816
26.    Patra JK, Baek K-H. Green synthesis of silver chloride nanoparticles using Prunus persica L. outer peel extract and investigation of antibacterial, anticandidal, antioxidant potential. Green Chem Lett Rev. 2016;9(2):132-142. doi:10.1080/17518253.2016.1192692
27.    Patil MP, Seo YB, Kim G Do. Morphological changes of bacterial cells upon exposure of silver-silver chloride nanoparticles synthesized using Agrimonia pilosa. Microb Pathog. 2018;116:84-90. doi:10.1016/j.micpath.2018.01.018
28.    Providence BA, Chinyere AA, Ayi AA, Charles OO, Elijah TA, Ayomide HL. Green synthesis of silver monometallic and copper-silver bimetallic nanoparticles using Kigelia africana fruit extract and evaluation of their antimicrobial activities. Int J Phys Sci. 2018;13 (3):24-32. doi:10.5897/ijps2017.4689

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