Author(s): Ashaq Hussain Rather, Rekha Rao

Email(s): drashaqhrather21919@gmail.com

DOI: 10.52711/0974-360X.2021.00342   

Address: Ashaq Hussain Rather1*, Rekha Rao2
1Algal Biotechnology Laboratory, Department of Post Graduate Studies and Research in Biological Sciences, Rani Durgavati University, Jabalpur - 482001, (M.P.), India.
2Regional Ayurveda Research Institute for Urinary Disorder, Jammu - 181123, (J&K), India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 4,     Year - 2021


ABSTRACT:
In the present investigation, effect of biomass, crude astaxanthin of Haematococcus pluvialis on the different hepatic and antioxidant enzymes against toxin, carbon tetrachloride (CCl4) induced toxicity on male Albino Wistar rat, weighing 200-220g which were compared with the commercially available astaxanthin and ascorbic acid all are mixed in liquid paraffin and feeding (orally) of 100µg/kg b.w of commercially available astaxanthin, biomass, crude extract, and ascorbic acid was started 2 weeks before to CCl4 treatment. The animals were sacrificed after 24 h of CCl4 treatment, collected samples, for the evaluation of hepatic and antioxidant enzymes Biomass (100µg/kg b.w) treated groups restored maximum damage induced by the CCl4, when results were compared with the toxin treated group. These results indicate that H. pluvialis biomass and crude extract showed umpteen hepatoprotective and antioxidant activity and thus can be used for various food formulations for human welfare.


Cite this article:
Ashaq Hussain Rather, Rekha Rao. Evaluation of Antioxidant and Hepatoprotective Activity of Biomass and Crude Astaxanthin extract of Green Alga Haematococcus pluvialis. Research Journal of Pharmacy and Technology. 2021; 14(4):1933-7. doi: 10.52711/0974-360X.2021.00342

Cite(Electronic):
Ashaq Hussain Rather, Rekha Rao. Evaluation of Antioxidant and Hepatoprotective Activity of Biomass and Crude Astaxanthin extract of Green Alga Haematococcus pluvialis. Research Journal of Pharmacy and Technology. 2021; 14(4):1933-7. doi: 10.52711/0974-360X.2021.00342   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-4-18


REFERENCES:
1. Higuera-Ciapara, I, Felix-Valenzuela L and Goycoolea, FM. Astaxanthin: A review of its chemistry and applications. Cri. Reviews in Food Sci. and Nutri. 2006; 46: 1851–96.
2. Johnson EA and An GH. Astaxanthin from Microbial Sources. Crit. Reviews in Biotechnol. 1991; 4: 297–326.
3. Ravinder P, Sangha K, Patel T and Martin AM. Growth and pigmentation in Rhodotorula rubra and Phqffia rhodozyma. J. Basic Microbiol. 1995; 35(l): 535–6.
4. Lee RG, Neamtu GG, Lee KC and Simpson KL. Pigmentation of rainbow trout with extracts of floral parts from Tagetes erecta and Circubita maxima marica. Rev. Roum. Biochem. 1978; 15(4): 287–293.
5. Bell ER and White EB. Flowers and plants as sources of food colourants. Intl. Ind. Biotechnol. 1989; 9(3): 609–614.
6. Kamata T, Tanaka Y, Yamada S and Simpson KL. Study of carotenoid composition and fatty acids of astaxanthin diester in rainbow trout (Salmo gairdneiri) fed the Adonis extract. Nippon. Suisan Gakkaishi. 1990; 56(5): 7897–94.
7. Jyonouchi H, Sun S and Gross M Effect of carotenoids on in vitro immunoglobulin production by human peripheral blood mononuclear cells: astaxanthin, a carotenoid without vitamin A activity, enhances in vitro immunoglobulin production in response to a T-dependent stimulant and antigen. Nutr. Cancer 1995; 23: 171–183.
8. Papas AM. Antioxidant Status, Diet, Nutrition, and Health, CRC Press 1999.
9. Palozza P and Krinsky NI Astaxanthin and canthaxanthin are potent antioxidants in a membrane model. Arch. Biochem. Biophys. 1992; 297: 291–295.
10. Naguib YMA. Antioxidant activities of astaxanthin and related carotenoids. J. Agric. Food Chem. 2000; 48: 1150–1154.
11. Miki W. Biological functions and activities of animal carotenoids; Pure and Appl. Chem. 1991; 63(1): 1411–46. 
12. Borowitzka MA, Huisman JM and Osborn A. Culture of the astaxanthin-producing green alga Haematococcus pluvialis 1. Effects of nutrients on growth and cell type J. Appl. Phycol. 1991; 3: 295–304.
13. Boussiba S and Vonshak A. astaxanthin Accumulation in the Green Alga Haematococcus pluvialis. Plant Cell Physiol. 1991; 32, 1077–1082.
14. Kobayashi M, Kakizono T and Nagai S. Astaxanthin production by a green algal, Haematococcus pluvialis accompanied with morphological changes in acetate media. J. Ferment. Bioeng. 1991; 71: 335–339.
15. Lee YK and Soh CW. Accumulation of astaxanthin in Haematococcus lacustris (Chlorophyta). J. Phyco. 1991; 27, 575–577.
16. Rather AH and Singh S Preliminary evaluation of impact of monochromatic light on the biosynthesis of astaxanthin in green alga Haematococcus pluvialis. World News of Nat. Sci. 2018; 19, 455–0.
17. Singh S and Rather AH. Impact of light and dark (L/D) period on the biosynthesis of astaxanthin in green alga Haematococcus pluvialis. J. App. Biol. Biotech. 2018; 6(06): 58–60.
18. Sarada R, Vidhyavathi R, Usha D and Ravishankar GA. An efficient method for extraction of astaxanthin from green alga Haematococcus pluvialis. J. of Agri. and Food Chem. 2006; 54: 7585–7588.
19. Ohkawa H, Ohishi N and Yagi K. Assay for Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction. Analytical Biochem. 1979; 95: 35l3–58.
20. Sapakal VD, Shikalgar TS, Ghadge RV, Adnaik RS, Naikwade NS and Magdum CS. In vivo Screening of Antioxidant Profile, A Review. J. of Herbal Medi. and Toxi.2008; 2 (2): 1–8.
21. Paoletti F, Aldinucci D, Mocali A and Caparrini A. A Sensitive Spectrophotometric Method for the Determination of Superoxide Dismutase Activity in Tissue Extracts. Anal Biochem 1986; 153: 536–541.
22. Ellman GL. Tissue Sulfydryl Groups. Arch. of Biochem. and Biophy. 1959; 82: 70–77.
23. Rao AR, Sarada R, Shylaja MD and Ravishankar GA Evaluation of hepatoprotective and antioxidant activity of astaxanthin and astaxanthin esters from microalga Haematococcus pluvial. J. of food sci. and tech. 2015; 52(10): 67036–710.
24. Jaeschke H, McGill MR and Ramachandran A. Oxidant stress, mitochondria, and cell death mechanisms in drug-induced liver injury: lessons learned from acetaminophen hepatotoxicity. Drug Metab. Rev. 2012; 44: 88–106.
25. Negre‐Salvayre A, Coatrieux C, Ingueneau C and Salvayre R. Advanced lipid peroxidation end products in oxidative damage to proteins. Potential role in diseases and therapeutic prospects for the inhibitors. Br. J. Pharmacol. 2008; 153: 6–20.
26. Sanda A, Gal A, Pintea A, Bedecean I, Arion A and Baba AI. Influence of astaxanthin administration on hepatic oxidative stress markers in rats injected with methylnitrosurea. Bull. UASVM. Vet.Med. 2008; 65(1): 1843– 5270.
27. Fassett RG and Combes JS. Astaxanthin: a potential therapeutic agent in cardiovascular disease. Mar. Drugs. 2011; 9: 447–465.
28. Turkez H, Geyikoglu F and Yousef MI. Beneficial effect of astaxanthin on 2, 3, 7, 8-tetrachloro dibenzo-p-dioxin-induced liver injury in rats. Toxicol. Ind. Health. 2012; 29: 591–599.
29. Kang JO, Kim SJ and Kim H. Effect of astaxanthin on the hepatotoxicity, lipid peroxidation and antioxidative enzymes in the liver of CCl4-treated rats. Methods Find Exp. Clin. Pharmacol. 2001; 23: 79–84.
30. Sindhu ER, Firdous AP, Preethi KC and Kuttan R. Carotenoid lutein protects rats from paracetamol, carbon tetrachloride and ethanol induced hepatic damage. J. Pharm. Pharmacol. 2010; 62:1054–1060
31. Hussein G, Goto H, Oda S, Sankawa U, Matsumoto K and Watanabe H. Antihypertensive potential and mechanism of action of astaxanthin: III. Antioxidant and histopathological effects in spontaneously hypertensive rats. Biol. Pharm. Bull. 2006; 29: 684–688.
32. Reagan-Shaw S, Nihal M and Ahmad N. Dose translation from animal to human studies revisited. FASEB J. 2008; 22: 6596–61.
33. Roche Vitamins Fine Chemicals. Astaxanthin as a pigmenter in salmon feed. http://www.fda.gov/ohrms/dockets/ dockets/95s0316/95s-0316-rpt0237-11Tab-I-vol172. 1987.

Recomonded Articles:

Author(s): Nimbalkar V.V., Pansare P.M., Nishane B.B.

DOI: 10.5958/0974-360X.2015.00310.8         Access: Open Access Read More

Author(s): U.S Mahadeva Rao, Khamsah Suryati Mohd, Abdurrazaq Muhammad, Bashir Ado Ahmad, Mohaslinda Mohamad, Rosmawati Mat Ali

DOI: Not Available         Access: Open Access Read More

Author(s): Deepak Karki, Gururaj S. Kulkarni, Shivakumar Swamy, Sheeba FR

DOI: 10.5958/0974-360X.2017.00750.8         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Hayat M. Mukhtar, Vandna Kalsi

DOI: 10.5958/0974-360X.2018.00395.5         Access: Open Access Read More

Author(s): Som K. Madhvi, Manik Sharma, Javaid Iqbal, Mohd Younis

DOI: 10.5958/0974-360X.2019.00785.6         Access: Open Access Read More

Author(s): Praveen D, Ranadheer Chowdary P, Gandikota Thanmayi, Gangabathina Poojitha, Vijey Aanandhi M

DOI: 0.5958/0974-360X.2016.00047.0         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): Fathima Mariyam Niyas

DOI: 10.5958/0974-360X.2015.00182.1         Access: Open Access Read More

Author(s): J.S. Vaghela, S.S. Sisodia

DOI: Not Available         Access: Open Access Read More

Author(s): Gopinath G, Thirumal M, P. R. Kumar

DOI: 10.5958/0974-360X.2020.00362.5         Access: Open Access Read More

Author(s): Ankita Chatterjee, Jayanthi Abraham

DOI: 10.5958/0974-360X.2016.00125.6         Access: Open Access Read More

Author(s): Trilokchandran. B, Vijayakumar G, Thippareddy K S

DOI: 10.5958/0974-360X.2019.00612.7         Access: Open Access Read More

Author(s): Krishnendu Acharya*, Somanjana Khatua, Salman Sahid

DOI: Not Available         Access: Open Access Read More

Author(s): Neha Rajput, Dinesh Sachan, Shikha Gangwar, Dipti Sachan

DOI: Not Available         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags