Author(s): Silvester Maximus Tulandi, Lisawati Tanzil, Dian Maria Ulfa


DOI: 10.5958/0974-360X.2021.00289.4   

Address: Silvester Maximus Tulandi*, Lisawati Tanzil, Dian Maria Ulfa
Department of Pharmaceutical and Food Analysis, Health Polytechnic Jakarta II, Ministry of Health, Jakarta, Indonesia.
*Corresponding Author

Published In:   Volume - 14,      Issue - 3,     Year - 2021

This study aimed to determine the bioactive component of gonads of the sea urchins Diadema setosum from Indonesian waters using Gas Chromatography Mass Spectrometry. Phytochemical test results qualitatively showed that the methanol extract of D. setosum gonads contained steroids-triterpenoids, saponins and alkaloids while the results of analysis using GC-MS illustrated that the largest contents were Hexadecanoic acid, methyl ester (29.53%) and Tetradecanoic acid, methyl ester (14.86%), followed by other components in small quantity including Methyl 13-methyltetradecanoate (0.55%), Pentadecanoic acid, methyl ester ss Methyl n-pentadecanoate (2.08%), Hexadecadienoic acid, methyl ester (0.48%), 9-Hexadecenoic acid, methyl ester (4.68%), Methyl 10-methyl-hexadecanoate (0.56%), Hexadecanoic acid (4.05%), Heptadecanoic acid, Methyl ester (4.17%), 6,9,12-Octadecatrienoic acid, Methyl ester (1.33%), 9-Octadecenoic acid (Z)-, Methyl ester (5.42%), Octadecanoic acid, Methyl ester (1.91%), 9,12-Octadecadienoic acid (Z, Z) -, Lineloic acid (8.58%) , 5,8,11,14-Eicosatetraenoic acid, Methyl ester (3.21%), 9,12-Octadecadienoic acid, Methyl ester (4.71%), Cis-11-Eicosenoic acid, Methyl ester (1.86%), (1S, 15S) -Byciclo [13.10] hexadecan-2-one (4.82%), Cholesta -3.5-diene (0.67%), Cholest-5-en-3-ol (3ß) - (4.20%). Components of the compound were reported to possess pharmacological function as antimicrobial, antifungal, antioxidant, anticancer, hipercholesterolemic agent, lubricant, antiinflammatory, nematicide, hepatoprotective, antihistaminic, pesticide, larvacidal activities, antiacne, anemiagenic, antiandrogenic, 5-alpha reduce inhibitor, antiarthritic and anticoronary properties.

Cite this article:
Silvester Maximus Tulandi, Lisawati Tanzil, Dian Maria Ulfa. Analysis of Bioactive Compounds from Methanol Extract of Diadema setosum Sea Urchin Gonads using Gas Chromatography – Mass Spectrometry. Research J. Pharm. and Tech 2021; 14(3):1629-1634. doi: 10.5958/0974-360X.2021.00289.4

Silvester Maximus Tulandi, Lisawati Tanzil, Dian Maria Ulfa. Analysis of Bioactive Compounds from Methanol Extract of Diadema setosum Sea Urchin Gonads using Gas Chromatography – Mass Spectrometry. Research J. Pharm. and Tech 2021; 14(3):1629-1634. doi: 10.5958/0974-360X.2021.00289.4   Available on:

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