Ranjitha Dhevi V. Sundar, Saranya Shankar, Lokesh Ravi, Sathiavelu Arunachalam
Ranjitha Dhevi V. Sundar1, Saranya Shankar1, Lokesh Ravi2, Sathiavelu Arunachalam1*
1School of Bio Sciences and Technology, VIT, Vellore - 632014, Tamil Nadu.
2Department of Botany, St Joseph’s College (Autonomous), Bengaluru, Karnataka, India.
Volume - 14,
Issue - 3,
Year - 2021
Aim: The study aimed to analyze the antibacterial, antioxidant and anti-inflammatory potential of two different extracts (Methanol and petroleum ether) of Annona reticulata leaves. Materials and Methods: Dried leaves powder of Annona reticulata was used to study the phytochemical analysis. Methanol and Petroleum ether extracts derived from leaves of A. reticulata were assessed for antioxidant by 2,2-diphenyl-1-picrylhydrazyl scavenging assay. Agar well diffusion method was used to study the antibacterial activity and in vitro anti-inflammatory activity was evaluated using the albumin denaturation method. And the extracts were subjected to gas chromatography-mass spectrometry (GC-MS) analysis to identify its bioactive components. Results: Qualitative phytochemical screening of leaf powder confirmed the presence of tannins, alkaloids, phenols, flavonoids and saponins. Both the extracts demonstrated significant antibacterial activity against the test organisms, methanol extract showed the highest inhibition zone of about 28mm at 10mg concentration Whereas in petroleum ether extract showed maximum against E.coli with a zone of inhibition of 25mm. Petroleum ether leaf extract demonstrated a maximum anti-inflammatory activity of 83.4% at 150µg/mL concentration and also demonstrated significant antioxidant activity in DPPH assay of 75% at 150µg/mL concentration. In silico docking studies revealed the interaction of ligands from petroleum ether extracts with the seven common antibacterial proteins.
Cite this article:
Ranjitha Dhevi V. Sundar, Saranya Shankar, Lokesh Ravi, Sathiavelu Arunachalam. Bioactivity of Annona reticulata-An in vitro and in silico Approach. Research J. Pharm. and Tech 2021; 14(3):1542-1548. doi: 10.5958/0974-360X.2021.00281.X
Ranjitha Dhevi V. Sundar, Saranya Shankar, Lokesh Ravi, Sathiavelu Arunachalam. Bioactivity of Annona reticulata-An in vitro and in silico Approach. Research J. Pharm. and Tech 2021; 14(3):1542-1548. doi: 10.5958/0974-360X.2021.00281.X Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-3-62
1. Kumar S, Paul S, Walia Y. K, Kumar A, Singhal P. Therapeutic Potential of Medicinal Plants: A Review. J. Biol. Chem. Chron 2015; 1: 46–54.
2. Singh R. Medicinal plants: A review. Journal of Plant Sciences 2015; 3: 50–55.
3. Acta A, Abdul B, Hassan R. Medicinal Plants (Importance and Uses). Pharmaceutica Analytica Acta 2012; 3.
4. Dogra K. S, Chauhan S, Jalal J. S. Assessment of Indian medicinal plants for the treatment of asthma. Journal of Medicinal Plants Research 2015; 9: 851–862.
5. Ravi L, V M, B, P. L. Antibacterial and Antioxidant Activity of Saponin From Abutilon Indicum Leaves. Asian Journal of Pharmaceutical and Clinical Research 2016; 9: 344–347.
6. Rani D. J, Rahinidevi R, Vidyashri M. Phytochemical screening and antimicrobial activity of various solvent extracts of Annona reticulata leaves. International Journal of Science Inventions Today 2013; 2: 347–358.
7. Kavitha M, Srinivasan P. T, Priya S. L, Jayakumar L. V. Evaluation of Nutrient Composition and Antibacterial Potential of Leaf and Stem of Mukia Maderaspatana L. International Journal of Pharmaceutical Research & Allied Sciences 2013; 2: 68–76.
8. Wadood A, Ghufran M, Jamal S. B, Naeem M, Khan A, Ghaffar R, Asnad. Biochemistry & Analytical Biochemistry Phytochemical Analysis of Medicinal Plants Occurring in Local Area of Mardan. Biochemistry and Analytical Biochemistry 2013; 2: 1–4.
9. Zaman K, Pathak K. Pharmacognostical and Phytochemical Studies on the Leaf and Stem Bark of Annona reticulata Linn.Journal of Pharmacognosy and Phytochemistry 2013; 1: 1–7.
10. Thang T. D, Kuo P, Huang G, Hung N. H, Huang B, Yang M, Wu T. Chemical Constituents from the Leaves of Annona reticulata and Their Inhibitory Effects on no Production. Molecules 2013; 18: 4477–4486.
11. DSVGK K, Rayavarapu K. A. Phytochemical analysis, antioxidant and antimicrobial activities of Annona reticulata raw fruit peel extracts. World Journal of Pharmacy and Pharmaceutical Sciences 2014; 3: 1226–1234.
12. Chandrashekar C, Kulkarni V. Isolation characterization and Antimicrobial activity of Annona squamosa leaf. Journal of Pharmacy Research 2011; 4: 1831–1832.
13. Nagham Mahmood Alijamali. Study effect of medical plant extracts in comparison with antibiotic against bacteria. Journal of Scientific and Innovative Research 2013; 2: 843–845.
14. Sundar R. D. V, Segaran G, Shankar S, Settu S, Ravi L. Bioactivity of Phoenix dactylifera seed and its phytochemical analysis. International Journal of Green Pharmacy 2017; 11: 1–6.
15. Subedi L, Timalsena S, Duwadi P, Thapa R, Paudel A, Parajuli K. Experimental study Antioxidant activity and phenol and flavonoid contents of eight me- dicinal plants from Western Nepal. Journal of Traditional Chinese Medicine 2014; 34: 584–590.
16. Sahu R. K, Kar M, Routray R. DPPH Free Radical Scavenging Activity of Some Leafy Vegetables used by Tribals of Odisha, India. Journal of Medicinal Plant Studies 2013; 1: 21–27.
17. De S, Das D.C, Mandal T. In vitro anti-inflammatory and anti-diabetic activity of Methanolic Extract of Cardanthera Difformis. International Research Journal of Pharmacy 2016; 7: 56–60.
18. Ravi L, Krishnan K. Cytotoxic Potential of N-hexadecanoic Acid Extracted from Kigelia pinnata leaves. Asian Journal of Cell Biology 2017; 12: 20–27.
19. Ravi L, Krishnan K. Benzoyloxy-ethyl-carbamic acid: A novel anticancerous secondary metabolite produced by Streptomyces globosus VITLGK011. Indian Journal of Experimental Biology 2017; 55: 411–420.