Author(s): V. C. Bhagat, M. S. Kondawar

Email(s): vishwasbhagat@rediffmail.com

DOI: 10.5958/0974-360X.2021.00270.5   

Address: V. C. Bhagat1*, M. S. Kondawar2
1Research Scholar, Appasaheb Birnale College of Pharmacy, Sangli, Maharashtra, India.
2Department of Quality Assurance, Appasaheb Birnale College of Pharmacy, Sangli. Maharashtra, India-416416.
*Corresponding Author

Published In:   Volume - 14,      Issue - 3,     Year - 2021


ABSTRACT:
The present study explored GC-MS analysis and in vitro antioxidant cytotoxicity study of dichloromethane: methanol (DCM-ME) extract of Dendrophthoe falcata plant and fractions (DFDM I-III) against human chronic myelogenous leukemia, bone marrow (k-562) Human lung carcinoma (A-549) cell lines by MTT and SRB cell viability assay method. Phytochemical screening of DCM-ME extract shows the presence of secondary metabolites such as alkaloids, flavonoids, saponins, tannins, sterols and triterpenes. DCM-ME extract shows cell inhibition 84.15±0.12% and 86.11±0.52% at 80µg/ml (IC50 values 20µg/ml, GI50 <20µg/ml) by MTT and SRB assay respect to Cisplatin (IC50<7.5µg/ml), Adriamycin (GI50 <2.5µg/ml). DFDM-I fraction shows significant effect (p< 0.01) with maximum cell inhibition activity 43.93±0.88%, and at 20µg/ml shows moderate activity (p<0.05) with cell inhibition 60.11±0.33% by MTT assay. SRB assay shows that DFDM-I at (10µg/ml) shows significant effect (p<0.01) with 47.72±0.33%, and at 20µg/ml shows moderate effect (p<0.05) with cell inhibition 65.15±0.58%. DCM-ME extract shows cell inhibition 70.72±1.15 at 80µg/ml (IC50 values 19.39µg/ml) against A-549 cell lines DFDM-I shows moderate effect (p<0.05) 51.56± 1.15 at 20µg/ml by MTT assay. Antioxidant activity evaluated by DPPH free radical scavenging activity found to be 87.27±3.76% for extract and 83.02±1.15, 78.03±2.49 76.03±2.33% for fractions respectively and 98.26±2.56% for ascorbic acid. GC-MS analysis DFDM-I fractions by Agilent 7890A GC coupled with Agilent triple quadrupole mass detector shows that the presence of the 17 phytocompounds. The major bioactive compounds of DFDM-I fractions were separated and identified as 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (27.0%), 6,10,14-trimethyl-2-Pentadecanone (52.8%), Hexadecanoic acid, methyl ester (56.4%),1,1-Diphenyl-4-phenylthiobut-3-en-1-ol (40.8%), Hexadecanoic acid butyl ester (57.6%), and 1-Monolinoleoylglycerol trimethylsilyl ether (35.1%). The study showed that the presence phytochemicals in the leaves extracts of D. falcata might be responsible for cell inhibitory potential against k-562 cell lines.


Cite this article:
V. C. Bhagat, M. S. Kondawar. GC-MS analysis and in vitro Antioxidant, Cytotoxicity study of DCM-ME extract of Dendrophthoe falcata (L.F) Ettingsh leave against human lung carcinoma (A-549) and human Chronic Myelogenous leukemia (k-562) cell Line. Research J. Pharm. and Tech 2021; 14(3):1521-1529. doi: 10.5958/0974-360X.2021.00270.5


REFERENCES:
1.    Kayser O, Kidderlen A F, Croft S L. Natural products as potential anti-parasitic drugs. Acta. Trop. 2000; 77, 307–314.
2.    Raskin I, Ribnicky DM, Komarnytsky S, Ilic N, Poulev A, Borinker N, Moreno DA, Ripoll C, Yakoby N. Plants and human health in the twenty-first century. Trends Biotechnology. 2002; 20, 522–531.
3.    Reddy L, Odhav B, Bhoola KD, Natural products for cancer prevention: a global perspective. Pharmacol. Ther. 2003; 99, 1–13.
4.    Saxena H O, Brahmam M. The Flora of Orissa. Capital Business Services and Consultancy, India, 1995; 1578–1580.
5.    Pattanayak SP, Sunita P. Wound healing, anti-microbial and antioxidant potential of Dendrophthoe falcata (L.f.) Ettingsh. J Ethnopharmacol. 2008; 120: 241e247.
6.    Bhattarai NK, Folk herbal medicines of Makawanpur district, Nepal. International Journal of Pharmacognosy.1991; 29, 284–295.
7.    Allekutty NA, Srinivasan KK, Gundu RP, et,al. Diuretic and antilithiatic activity of Dendrophthoe falcata. Fitoterapia. 1993; 64, 325–331.
8.    Mary KT, Kuttan R, Kuttan G. Cytotoxicity and immunomodulatory activity of Loranthus extract. Amala Research Bulletin. 1993; 13, 53–58.
9.    Nair AGR, Krishnakumary P. Flavonoids from Dendrophthoe falcata Ettingsh growing on different host plants. Indian Journal of Chemistry. 1989; 29, 584–585.
10.    Mallavadhani U, Narashimhan K, Mohapatra A, Breeman RB. New pentacyclic triterpenes and some flavanoids from the fruits of Indian ayurvedic plant Dendrophthoe falcata and their receptor binding activity. Chemical Pharmaceutical Bulletin. 2006; 54, 740–744.
11.    R.S. Gupta, J.B.S. Kachhawaa. Evaluation of contraceptive activity of methanol extract of Dendrophthoe falcata stem in male albino rats Journal of Ethnopharmacology.2007; 112, 215–218
12.    Mudgal G, Mudgal B, Arch. Phytopathol. Plant Prot.2011; 44, 186–190.
13.    Trease G E, Evans WC, A Text Book of Pharmacognosy. ELSB Baillere Tindal, Oxford, 1987;
14.    Harborne J B Phytochemical Methods, A Guide to Modern Techniques of Plant Analysis, 3rd ed. Springer Pvt. Ltd., New Delhi, India. 1998
15.    Wagner H, Bladt, Plant Drug Analysis, A thin layer chromatography Atlas, 2nd Edn. Springer-Verlag, Berlin Heidelberg London, New York, 1996; 349–354.
16.    RS Policegoudra, P. Chattopadhyay S M, Aradhya R R, Shiva swami L Singh, VVeer. Inhibitory effect of Tridax procumbens against human skin pathogens R. Journal of Herbal Medicine. 2014; 01.004.
17.    CA Ukwubile, A Ahmed, UA Katsayal, J. Ya’u, S Mejida. GC–MS analysis of bioactive compounds from Melastomastrum capitatum (Vahl) Fern. Leaf methanol extract. An anticancer plant. Scientific African. 2019; 3, e0059.
18.    Mosmann T. Rapid colonmetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 1983; 65, 55-63.
19.    Alan Dolly, J Brayan Griffiths. John Willey and Son’s editors. Textbook of ‘Cell and Tissue Culture for Medical Research. John Wiley and Sons Publishers, 2003; 62-64.
20.    Joseph M. Nguta, Regina Appiah-Opong, Alexander K. Nyarkoet, et.al; 2016. In vitro antimycobacterial and Cytotoxic data on medicinal plants used to treat.
21.    Skehn P, Storeng R, Scudiero A et, al. New colorimetric cytotoxicity assay for anticancer drug screening J. Natl. Cancer Inst., 1990; 82, 1107.
22.    Vanicha Vichai, Kanyawim Kirtikara. Sulforhodamine B colorimetric assay for cytotoxicity screening, Nature Protocols. 2006; 1, 1112 - 1116.
23.    Erum Iqbal, Kamariah Abu Salim, Linda B.L. Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus (Airy Shaw) from Brunei Darussalam. Journal of King Saud University – Science, 2015; 27, 224–232.
24.    Stankovic M.S. Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac J. Sci. 2011; 33, 63–72.
25.    Benbott A, Yahiya A, Belaıdi A. Assessment of the antibacterial activity of crude alkaloids extracted from seeds and roots of the plant Peganum harmala L. J. Nat. Prod. Plant Resour. 2012; 2, 568–573.
26.    Ameyaw Y, Duker-Eshun G. The alkaloid contents of the ethno-plant organs of three antimalarial medicinal plant species in the eastern region of Ghana. Int. J. Chem. Sci., 2009; 7, 48–58.
27.    Kumari M, Jain S. Review paper, Tannins: an ant nutrient with positive effect to manage diabetes. Res. J. Recent Sci., 2012; 1, 70–73.
28.    Mayank Thakur, Matthias F Melzig, Hendrik Fuchs, Alexander Weng. Chemistry and pharmacology of saponins: special focus on cytotoxic properties. Botanics: Targets and Therapy, 2011;1.
29.    Harada H, U Yamashita, H Kurihara, F Fukushi, J Kawabata and Y. Kamei. Antitumor activity of palmitic acid found as a selective cytotoxic substance in a marine red alga. Anticancer Res., 2002; 22: 2587-2590. 26
30.    Hsiao Chi Wang, Jen Hung Yang, Shu Chen Hsieh, Lee Yan Sheen. Allyl Sulfides inhibit cell growth of skin cancer cells through induction of DNA damage mediated G2/M arrest and apoptosis. J. Agric. Food Chem. 2010; 58, (11): 7096-7103

Recomonded Articles:

Author(s): Rupali Deshmukh, Roshni Agrawal, Sarita Chauragde, Swati Lilhare, M. U. Mishra

DOI: 10.5958/0974-360X.2018.00831.4         Access: Open Access Read More

Author(s): Koushika Das, Pranit Krishna, Avipsha Sarkar, Shanmuga Sundari Ilangovan, Shampa Sen

DOI: 10.5958/0974-360X.2017.00267.0         Access: Open Access Read More

Author(s): U.S Mahadeva Rao, Khamsah Suryati Mohd, Abdurrazaq Muhammad, Bashir Ado Ahmad, Mohaslinda Mohamad, Rosmawati Mat Ali

DOI: Not Available         Access: Open Access Read More

Author(s): Deepak Karki, Gururaj S. Kulkarni, Shivakumar Swamy, Sheeba FR

DOI: 10.5958/0974-360X.2017.00750.8         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Hayat M. Mukhtar, Vandna Kalsi

DOI: 10.5958/0974-360X.2018.00395.5         Access: Open Access Read More

Author(s): Praveen D, Ranadheer Chowdary P, Gandikota Thanmayi, Gangabathina Poojitha, Vijey Aanandhi M

DOI: 0.5958/0974-360X.2016.00047.0         Access: Open Access Read More

Author(s): Som K. Madhvi, Manik Sharma, Javaid Iqbal, Mohd Younis

DOI: 10.5958/0974-360X.2019.00785.6         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): Fathima Mariyam Niyas

DOI: 10.5958/0974-360X.2015.00182.1         Access: Open Access Read More

Author(s): Surendra Nath Pandeya, Rajeev Kumar, Arun Kumar, Ashish Kumar Pathak

DOI: Not Available         Access: Open Access Read More

Author(s): J.S. Vaghela, S.S. Sisodia

DOI: Not Available         Access: Open Access Read More

Author(s): Krishnendu Acharya*, Somanjana Khatua, Salman Sahid

DOI: Not Available         Access: Open Access Read More

Author(s): Anket Sharma, Vinod Kumar, Parminder Kaur, Ashwani Kumar Thukral, Renu Bhardwaj

DOI: 10.5958/0974-360X.2015.00299.1         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags