Author(s): Anju Elza Joseph, Shajan Abraham, Steffy P Raju, Haritha H Pillai, Feba Jose, Elessy Abraham

Email(s): steffypallithadathil@gmail.com

DOI: 10.5958/0974-360X.2021.00265.1   

Address: Anju Elza Joseph1*, Dr. Shajan Abraham2, Steffy P Raju3, Haritha H Pillai4, Feba Jose5, Dr. Elessy Abraham6
1M. Pharm Delegate, Nazareth College of Pharmacy, Othera P.O Thiruvalla.
2Professor, Nazareth College of Pharmacy, Othera P.O Thiruvalla.
3M. Pharm Delegate, Nazareth College of Pharmacy, Othera P.O Thiruvalla.
4M. Pharm Delegate, Nazareth College of Pharmacy, Othera P.O Thiruvalla.
5M. Pharm Delegate, Nazareth College of Pharmacy, Othera P.O Thiruvalla.
6Principal, Nazareth College of Pharmacy, Othera P.O Thiruvalla.
*Corresponding Author

Published In:   Volume - 14,      Issue - 3,     Year - 2021


ABSTRACT:
Levofloxacin is an antibacterial agent used to treat various urinary tract infection as well as pneumonia. Levofloxacin was formulated as liposome using soya lecithin and cholesterol by thin film hydration method. It is then formulated as stealth liposome to increase the efficacy and reduce the toxicity and target the organs RES and thus prolonging its circulation time thereby reducing clearance. Two polymers are used to prepare Levofloxacin as stealth liposome i.e., PEG and PVP and their effects are compared. The formulated liposomes and stealth liposomes were evaluated for various parameters like surface morphology, zeta potential, poly dispersity index, drug content, % drug encapsulation and invitro drug release. The optimized formulation (F2) containing minimum concentration of cholesterol showed moderate stability and good entrapment efficiency. The stealth liposomes showed more stability and sustained drug release for 24hr compared to conventional liposomes. The size of stealth liposomes was slightly enhanced compared to conventional liposomes due to the polymers forming thick surface layer on surface of the vesicles. When compared to conventional liposomes, stealth liposomes showed more zeta potential values due to the effect of PEG 4000 and PVP K30.The value of zeta potential showed that stealth liposomes had sufficient charge to inhibit aggregation of liposomes due to electric repulsion. The kinetic data analysis of formulations indicated that it fits to Higuchi model and follows zero order release kinetics. The mechanism of drug release from the stealth liposomes followed non-fickian diffusion. The stability studies of the optimized stealth liposome were subjected as per ICH guidelines.


Cite this article:
Anju Elza Joseph, Shajan Abraham, Steffy P Raju, Haritha H Pillai, Feba Jose, Elessy Abraham. Formulation and Evaluation of Levofloxacin Stealth Liposome using different Polymers. Research J. Pharm. and Tech 2021; 14(3):1493-1498. doi: 10.5958/0974-360X.2021.00265.1

Cite(Electronic):
Anju Elza Joseph, Shajan Abraham, Steffy P Raju, Haritha H Pillai, Feba Jose, Elessy Abraham. Formulation and Evaluation of Levofloxacin Stealth Liposome using different Polymers. Research J. Pharm. and Tech 2021; 14(3):1493-1498. doi: 10.5958/0974-360X.2021.00265.1   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-3-54


REFERENCES:
1.    Reddy PD, Swarnalatha D. Recent advances in novel drug delivery Systems. Int J Pharm Tech Res. 2010; 25-27.
2.    Available from: https://www.pharmatutor.org/articles/sustainedrelease drug delivery system concise review. [Last accessed on May 2019 16th]
3.    Shailesh S, Neelam S, Sandeep K, Gupta GD. Liposomes: A review. J Pharm Res. 2009; 2(7): 1163-67.
4.    Wasankar SR, Faizi SM, Deshmuk AD. Formulation and development of liposomal gel for topical drug delivery system. Int J Pharm Sci Res. 2012;3(11): 4461-4470.
5.    Immordino ML, Dosio F, Cattel L. Stealth liposomes: Review of the basic science, rationale and clinical applications, existing and potential. Int J Nanomed. 2006; 1(3): 297–315.
6.    Sahil K, Premjeet S, Ajay B, Middha A, Bhawna K. Stealth liposomes: A review. Int J Res Ayurveda Pharm.2011; 2(5): 1534-38.
7.    Assanhou GA, Alolga NR, Onoja V, Agbokponto EJ, Kassim AS, Sabi-mouka EM. Polymers used for surface modifications in stealth liposomes preparations: A review. World J of Pharma Res.2015; 4(4): 2064-86.
8.    Available from: en.wikipedia.org/wiki/Quinolone antibiotic. [Last accessed on April 2019 20th]
9.    Indian Pharmacopoeia 1996 Vol 2
10.    Available from: https://en.wikipedia.org/wiki/meltingpoint. [Last accessed on April 2019 2nd]
11.    Available from: https://Shodhganga.ac.in/bitstream/10603/9423/12/12chapter [Last accessed on April 2019 4th]
12.    Shahwal VK, Dubey BK, Bhoumick M. Preformulation study of Levofloxacin. Int J Adv Pharm.2012; 1(1): 1-8
13.    Begum MY, Abbulu K, Sudhakar M. Flurbiprofen-loaded stealth liposomes: Studies on the development, characterization, pharmacokinetics and biodistribution. J Young Pharm.2012; 4(4): 209-19.
14.    Prabhu P, Shetty R, Koland M, Vijayanarayana, K, Vijayalakshmi KK, Nairy HM and Nisha GS. Investigation of Nano lipid vesicles of Methotrexate for anti-rheumatoid activity. Int J Nanomed.2012; 7: 177-86.
15.    Mansoori MA, Jawade S., Agrawal S, Khan MI. Formulation development of Ketoprofen liposomal gel. Int J Pharma Cosmetol.2012; 2(10): 23-9.

Recomonded Articles:

Author(s): Niha Naveed, Karthikeyan Murthykumar, Subasree Soundarajan, Sripradha Srinivasan

DOI: Not Available         Access: Open Access Read More

Author(s): Umesh Kumar, Rakesh Narang, Surendra Kumar Nayak, Sachin Kumar Singh, Vivek Gupta

DOI: 10.5958/0974-360X.2017.00425.5         Access: Open Access Read More

Author(s): Rakhi Mehra, Renu Makhija, Neera Vyas

DOI: Not Available         Access: Open Access Read More

Author(s): Sandeep D. S, Prashant Nayak, Jobin Jose, Rishal Relita M, Sumana D. R.

DOI: 10.5958/0974-360X.2017.00317.1         Access: Open Access Read More

Author(s): Hayat M. Mukhtar, Vandna Kalsi

DOI: 10.5958/0974-360X.2018.00395.5         Access: Open Access Read More

Author(s): Gopinath G, Thirumal M, P. R. Kumar

DOI: 10.5958/0974-360X.2020.00362.5         Access: Open Access Read More

Author(s): Dhanalekshmi UnniKrishnan Nair, MP Saraswathy, Narra Kishore, Neelakanta Reddy Pully

DOI: Not Available         Access: Open Access Read More

Author(s): L. Srinivas, V. Manikanta, M. Jaswitha

DOI: 10.5958/0974-360X.2019.00230.0         Access: Open Access Read More

Author(s): Amutha K*, Ashok Kumar A, Godavari A

DOI: 10.5958/0974-360X.2017.00100.7         Access: Open Access Read More

Author(s): Yasmin A, Vijaya Bharathi R, Radha R

DOI: 10.5958/0974-360X.2019.00429.3         Access: Open Access Read More

Author(s): S. Syed Shihaab E Noor, Pradeep

DOI: 10.5958/0974-360X.2016.00353.X         Access: Open Access Read More

Author(s): R. Manikandan, A. Vijaya Anand

DOI: 10.5958/0974-360X.2015.00056.6         Access: Open Access Read More

Author(s): Moghal. Roohi Shabreen, S. Sangeetha

DOI: 10.5958/0974-360X.2020.00355.8         Access: Open Access Read More

Author(s): S. Krishnakumar, R. Divya, N.R. Kanchana Devi, G. Keerthana, A. Ancy Judi

DOI: 10.5958/0974-360X.2017.00234.7         Access: Open Access Read More

Author(s): A. Khuntia, S.K. Mohanty

DOI: Not Available         Access: Open Access Read More

Author(s): Pankaj Kumar, Jennifer Fernandes, Abhishek Kumar

DOI: 10.5958/0974-360X.2017.00023.3         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags