Rima Khalil, Moufid Yassin, Sundus J. Yaseen
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Rima Khalil1, Moufid Yassin2, Sundus J. Yaseen3
1Master Student in Analytical and Food Chemistry Department, Faculty of Pharmacy, Tishreen University, Latakia, Syria and a Member of a Technical Committee at the University of Kalamoon University, Deir Atieh, Syria.
2Professor in Analytical and Food Chemistry Department, Faculty of Pharmacy, Tishreen University, Latakia, Syria.
3Doctor in Biochemistry and Microbiology, Faculty of Pharmacy, Kalamoon University, Deir Atieh, Syria.
Volume - 14,
Issue - 3,
Year - 2021
Aflatoxins are toxic secondary metabolites which are produced by some fungi that invade many different crops before and after harvest especially peanuts. In this study twenty samples of raw peanut kernels were randomly collected from different supermarkets in Latakia city, Syria for detection of their contamination with aflatoxins. The levels of aflatoxins in collected samples were determined using high performance liquid chromatography equipped with post-column photochemical reactor for enhanced detection and fluorescence detector. Extraction and cleanup of aflatoxins were achieved by 70% methanol and immunoaffinity column (IAC). A detection limit of (0.02-0.04ng/ml) and a quantification limit of (0.06-0.132 ng/ml) were obtained for the studied types of aflatoxins. The linearity was in the range (0.156-20ng/ml) for AFB1, AFB2, AFG1 and AFG2. 55% and 25% of the samples were contaminated with Total aflatoxins and AFB1, respectively. The concentrations in all investigated samples ranged from not detected to 149.96µg/kg with mean concentration ±SD (16.49±34.08 µg/kg) for total aflatoxins and from not detected to 37.10µg/kg with mean ±SD (3.46±8.94µg/kg) for AFB1. 25% and 50% of tested samples exceeded the maximum acceptable limits set by the European commission.
Cite this article:
Rima Khalil, Moufid Yassin, Sundus J. Yaseen. Detection some Aflatoxins in some locally marketed Raw Peanuts. Research J. Pharm. and Tech 2021; 14(3):1431-1437. doi: 10.5958/0974-360X.2021.00255.9
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