Author(s): M. Sabareesh, J.P. Yanadaiah, K.B. Chandra Sekhar

Email(s): sabareesh85@gmail.com

DOI: 10.5958/0974-360X.2021.00254.7   

Address: M. Sabareesh*1, J.P. Yanadaiah2, K.B. Chandra Sekhar3
1Research Scholar, Jawaharlal Nehru Technological University, Anantapur, Ananthapuramu, Andhra Pradesh, India.
2Professor, Dr. K.V. Subba Reddy Institute of Pharmacy, Kurnool, Andhra Pradesh, India.
3Professor, Department of Chemistry, Krishna University, Machilipatnam, Andhra Pradesh, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 3,     Year - 2021


ABSTRACT:
The transdermal nanoproniosomal gel of Losartan potassium was prepared to treat hypertension that is efficient to deliver the encapsulated drug over extended periods and to provide better bioavailability. In the present study, the nanoproniosomal gel of Losartan potassium was formulated by Lecithin, Cholesterol, Non-ionic surfactants using the Coacervation-phase separation method. The physical mixture of drug, lecithin, and cholesterol were subjected to compatibility study using FTIR spectroscopy. The prepared nanoproniosomal gels were subjected to various evaluation parameters like the determination of pH and viscosity, vesicle size analysis, rate of spontaneity, entrapment efficiency, zeta potential, ex vivo skin permeation studies, skin irritation test, stability studies and in vivo antihypertensive studies. The physical characterization of nanoproniosomal gels was found to be within the acceptable limits. The ex vivo skin permeation studies showed the cumulative permeation of 47.25 % to 82.49% through the albino rat skin in 24 hrs for all the formulations which indicate the zero-order drug permeation with diffusion, non-fickian release as the possible mechanisms of drug release. Among all formulations, NLPG2 was selected as best formulation because it showed better characteristics than other formulations in several aspects like entrapment efficiency, vesicle size, ex vivo permeation studies, zeta potential, stability studies, and other evaluation parameters. The selected formulation NLPG2 showed the highest transdermal flux (28.67µg/cm2/h), with an enhancement ratio of 2.87 when compared to other formulations. In vivo antihypertensive study revealed that the formulation NLPG2 was successful to regress the rat BP to normal values in experimental hypertensive rats. Finally, it could be concluded that the formulation NLPG2 accentuates the flux of Losartan potassium and is an efficient transdermal therapeutic system for the delivery of a drug. The nanoproniosomal gels are suitable for once a day controlled release formulation.


Cite this article:
M. Sabareesh, J.P. Yanadaiah, K.B. Chandra Sekhar. Formulation Development, Ex vivo Evaluation and In vivo Antihypertensive study of Losartan Potassium Loaded Nanoproniosomal Gel: A Novel Vesicular Approach for Transdermal Delivery. Research J. Pharm. and Tech 2021; 14(3):1423-1430. doi: 10.5958/0974-360X.2021.00254.7


REFERENCES:
1.    Radha GV, Sudha Rani T, Sarwani B. A review on proniosomal drug delivery system for targeted drug action. Journal of Basic and Clinical Pharmacy. 2013; 4 (2): 42-48.
2.    Jangam Payal R, Thombre Nilima A, Gaikwad Pallavi N. A Review: Proniosomes as a Novel Drug Delivery System. Asian J. Pharm. Tech. 2017; 7 (3): 166-174.
3.    Srikanth, Anand Kumar Y, Mallikarjuna Setty C. Formulation and Evaluation of Maltodextrin Based Doxorubicin HCl Proniosomes. Research J. Pharm. and Tech. 2019; 12 (6): 2776-2780.
4.    Srikanth K, Nappinnai M and Gupta VRM. Proniosomes: A Novel Drug Carrier System. Research J. Pharm. and Tech. 2010; 3 (3): 709-711.
5.    Sharda Sambharkar, Sarvesh Paliwal, Swapnil Sharma, Bishambar Singh. Formulation of risperidone loaded proniosomes for effective transdermal delivery: An in vitro and in vivo study. Bulletin of Faculty of Pharmacy, Cairo University. 2017; 55: 239-247.
6.    Maryam Khatoon, Kiyafat Ullah Shah, Fakhar Ud Din, Shefaat Ullah Shah, Asim Ur Rehman, Naz Dilawar et al. Proniosomes derived niosomes: Recent advancements in Drug Delivery and targeting. Drug Delivery. 2017; 24 (2): 56-69.
7.    Kondawar MS, Kamble KG, Malusare MK, Waghmare JJ and Shah ND. Proniosome Based Drug Delivery System for Clotrimazole. Research J. Pharm. and Tech. 2011; 4 (8): 1284-1287.
8.    Ankur Gupta, Sunil Kumar Prajapati, Bala Murugan M, Mamta Singh, Daksh Bhatia. Design and development of a proniosomal transdermal drug delivery system for captopril. Trop J Pharm Res. 2007; 6 (2): 687-693.
9.    Waghmode maya and Shruthi Ashar. Proniosomal drug delivery systems: An overview. International Journal of Pharmaceutical and Chemical Sciences. 2012; 1 (3): 1044-1056.
10.    Gopala Krishna Murthy T.E., Sai Kishore V. Formulation and Evaluation of Transdermal Gels of Diltiazem Hydrochloride. Indian Journal of Pharmaceutical Education and Research. 2008; 42 (3): 272-276.
11.    Umekar M.J., Biyani D.M., Amgaonkar Y.M., Bhoyar P.K., Lade U.B. and Kalsait R.P. Formulation Development and Evaluation of Transdermal Drug Delivery System of Antihypertensive Drug. Research J. Pharm. and Tech. 2010; 3 (3): 753-757.
12.    www.drugbank.ca/drugs/DB00678
13.    www.drugs.com/losartan.html
14.    Naga Raju K, Sudheer Babu I, Sunitha T, Sai Ram P. Spectrophotometric Methods for Estimation of Losartan Potassium in Bulk Drug and Its Dosage Form. Asian J. Research Chem. 2012; 5 (3): 355-357.
15.    Nalanda T. Rangari, Prashant K. Puranik, Sanjay R. Chaudhari. Spectrophotometric determination of losartan potassium in pure form and in tablet dosage form. International Journal of Pharm Tech Research. 2015; 8 (1): 142-145.
16.    Rao PLKM, Venugopal V, Anil Kumar G, Rajesh B, Prasad GAL and Ravinder Goud. Quantitative estimation of losartan potassium in pharmaceutical dosage forms by UV spectrophotometry. International Journal of Research in Pharmacy and Chemistry. 2011; 1 (3): 295-302.
17.    Rupali Kirtawade, Pallavi Salve, Chhotaram Seervi, Kiran Patil, Anita Kulkarni and Pandurang Dhabale. Simultaneous UV-Spectrophotometric Method for Estimation of Atenolol and Losartan Potassium in Tablet Dosage Form. Asian J. Research Chem. 2010; 3 (4): 1050-1053.
18.    Prem Kumar Y, Vinod Kumar K, Sai Kishore V. Preparation and Evaluation of Diclofenac Niosomes by Various Techniques. Research J. Pharm. and Tech. 2013; 6 (10): 1097-1101.
19.    Venkata Ramesh Yasam, Satya Lavanya Jakki, Jawahar Natarajan, Senthil Venkatachalam, Gowthamarajan Kuppusamy, Sumeet Sood et al. A novel vesicular transdermal delivery of nifedipine- preparation, characterization and in vitro/in vivo evaluation. Drug Deliv. 2014; 23 (2): 619-630.
20.    Ming Ming Wen, Ragwa M. Farid, and Abeer A. Kassem. Nano-proniosomes enhancing the transdermal delivery of mefenamic acid.  J Liposome Res. 2014; 24 (4): 280-289.
21.    Viney Lather, Dharmpal Sharma and Deepti Pandita. Proniosomal gel- mediated transdermal delivery of bromocriptine: in vitro and ex vivo evaluation. Journal of Experimental Nanoscience. 2016; 11 (13): 1044-1057.
22.    Nadeem Farooqui, Mousumi Kar, Ravindra Pal Singh. Formulation and development of proniosomal gel for transdermal delivery of ketorolac tromethamine. Asian Journal of Pharmaceutics. 2016; 10 (3): S394-S400.
23.    Rishu Kakkar, Rao Rekha, Dahiya Navin Kumar and Nanda Sanju. Formulation and characterisation of valsartan proniosomes. Maejo Int. J. Sci. Technol. 2011; 5 (01): 146-158.
24.    Ashish Kute, Prakash Goudanavar, Doddayya Hiremath, S.R. Reddy. Development and Characterization of Perindopril Erbumine Loaded Proniosomal Gel. Asian J. Pharm. Tech. 2012; 2 (2): 54-58.
25.    Sandeep Gupta, Dheeraj Ahirwar, Neeraj K Sharma, and Deenanath Jhade. Proniosomal Gel as a Carrier for Improved Transdermal Delivery of Griseofulvin: Preparation and In-Vitro Characterization. Research J. Pharma. Dosage Forms and Tech. 2009; 1 (1): 33-37.
26.    OECD Guidelines for Testing of chemicals. 2015. Available from: https://www.oecd.org/env/test-no-404-acute-dermal-irritation-corrosion-9789264242678-en.htm
27.    Singh S, Trivedi S, Jain S. Design and development of proniosome based transdermal delivery of ondansetron hydrochloride. International Journal of Pharmaceutical and Biological Research. 2012; 3 (5): 191-201.
28.    Faiyaz Shakeel, Sanjula Baboota, Alka Ahuja, Javed Ali, Mohammed Aqil and Sheikh Shafiq. Nanoemulsions as Vehicles for Transdermal Delivery of Aceclofenac. AAPS Pharm Sci Tech. 2007; 8(4): E1-E9. (http://www.aapspharmscitech.org).
29.    Sadashivaiah R, Dinesh BM, Uma A Patil, Desai BG, Raghu KS. Design and in vitro evaluation of haloperidol lactate transdermal patches containing ethyl cellulose-povidone as film polymers. Asian Journal of Pharmaceutics. 2008; p. 43-49.
30.    M. Sabareesh, J.P. Yanadaiah, K.B. Chandra Sekhar. A Novel Vesicular Approach for Transdermal Administration of Enalapril Maleate Loaded Nanoproniosomal Gel: Formulation, Ex Vivo Evaluation and In Vivo Antihypertensive Study. International Journal of Applied Pharmaceutics. 2020; 12 (5): 190-202.
31.    Abdul Ahad, Mohd. Aqil, Asgar Ali. Investigation of antihypertensive activity of carbopol valsartan transdermal gel containing 1,8-cineole. International Journal of Biological Macromolecules. 2014; 64: 144-149.

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