Author(s): Venkata Padmini M., Gowri Sankar D.

Email(s): padmajapriya39@gmail.com

DOI: 10.5958/0974-360X.2021.00252.3   

Address: Venkata Padmini M.*, Prof. Gowri Sankar D.
Department of Pharmaceutical Sciences, A. U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 3,     Year - 2021


ABSTRACT:
The present study was aimed to develop a novel, simple, rapid accurate and precise, stability-indicating reversed-phase Ultra-performance liquid chromatography method for the simultaneous estimation of Atazanavir and Cobicistat in bulk and tablet dosage forms.The chromatographic elution was achieved in isocratic mode using the combination of Atazanavir and Cobicistat in the ratio of 0.01N KH2PO4 and Acetonitrile (70:30%v/v) using a HHS C18 column which has specification (100× 2.1mm, 1.8µ) and the flow rate of 0.3ml/min and wavelength detection at 237.0nm. The retention time obtained for Atazanavir and Cobicistat was 1.309 min, 1.607 min respectively. Atazanavir and Cobicistat their combination drug product were exposed to acidic, alkali, thermal, photolytic, and oxidative stress conditions. The current method was validated according to the ICH guidelines for accuracy, precision, linearity, specificity, and sensitivity.


Cite this article:
Venkata Padmini M., Gowri Sankar D. Stability Indicating RP-UPLC Method for Simultaneous Estimation of Atazanavir and Cobicistat in Bulk and Tablet Dosage Forms. Research J. Pharm. and Tech 2021; 14(3):1412-1416. doi: 10.5958/0974-360X.2021.00252.3

Cite(Electronic):
Venkata Padmini M., Gowri Sankar D. Stability Indicating RP-UPLC Method for Simultaneous Estimation of Atazanavir and Cobicistat in Bulk and Tablet Dosage Forms. Research J. Pharm. and Tech 2021; 14(3):1412-1416. doi: 10.5958/0974-360X.2021.00252.3   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-3-40


REFERENCES:
1.    Atazanavir, Drug available at https://www.drugbank.ca/drugs/ DBO1072
2.    Cobicistat, Drug available at https://www.drugbank.ca/drugs/ DBO9065
3.    Devailal J, Narottam Pal, Sumalatha K, Srinivasa Rao A. New method development and validation for simultaneous determination of Atazanavir and Cobicistat in bulk and tablet dosage form by UPLC. Scholars Research Library. 2018; 10(6):1-9.
4.    Leela Madhuri D. Bio analytical method development and validation for simultaneous estimation of Atazanavir and Cobicistat by RP-HPLC in human plasma. World Journal of Pharmaceutical Sciences. 2016; 4(7): 2.
5.    Sindu Priya D, Gowri Sankar D. Stability indicating RP-HPLC method development and validation for simultaneous determination of Atazanavir and Cobicistat in bulk and pharmaceutical formulation. American Journal of Pharmatech Research.2018; 2249:3387.
6.    ICH guidelines Q2 (R1), Validation of Analytical procedures, Text and Methodology 1995.

Recomonded Articles:

Author(s): A. S. K. Sankar, B. Datchayani, N. Balakumaran, Mohammed Rilwan, R. Subaranjani

DOI: 10.5958/0974-360X.2017.00047.6         Access: Open Access Read More

Author(s): Peethala Prathyusha, Raja Sundararajan, Palyam Bhanu, Mathrusri Annapurna Mukthinuthalapati

DOI: 10.5958/0974-360X.2020.00507.7         Access: Open Access Read More

Author(s): V. Shirisha, Somsubhra Ghosh, B. Rajni, David Banji

DOI: Not Available         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Narendra Chotai, Vishnu Patel, Harsha Patel, Uren Patel, Rajendra Kotadiya

DOI:         Access: Open Access Read More

Author(s): Shrivastava Alankar, Jain R., Agrawal R.K., Ahirwar D.

DOI:         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj Charde, Rajesh Shukla, Rita M. Charde

DOI: Not Available         Access: Open Access Read More

Author(s): Mukta D. Naykode, Durgacharan A. Bhagwat, Swapnil D. Jadhav, Harinath N. More

DOI: 10.5958/0974-360X.2017.00133.0         Access: Open Access Read More

Author(s): Vijaykumar T. Pawar, Shubhangi V. Pawar, Harinath N. More, Anita S. Kulkarni, Dinanath T. Gaikwad

DOI: 10.5958/0974-360X.2017.00724.7         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags