Sixty sample for hospital auditors of medical city hospital laboratories were collected of burn, skin, urine, sinus, spinal fluid and blood samples. Twelve isolates of these samples were isolated and identified depends on cultural, morphological, biochemical and VITEK tests, the results indicated that these isolates belong to S. aureus bacteria. Further studies were performed to determine their susceptibility for antibiotics involved Methicillin, Oxacillin, Bacitracin, Aztreanam and Hippurate. The isolates appeared varied susceptibility against these antibiotics, when they appeared three isolates out of eleven were resist to methicillin and oxacillin, while the all isolates were resist to other antibiotics. The three resist isolates subjected to antibacterial agents of turmeric and verbena oils with different concentrations of 25, 50, 75 and 100%, to determine their susceptibility against these substances, the results indicated these isolates were resistant to these oils. The same three isolates that were gained multi resist to antibiotics and oils was subjected to determine their MIC against oxacillin by using VITEK kit, and the results appeared that these isolates was resist to this antibiotic with MIC equal to = 4. Also the genomic DNA was extracted, then seven of housekeeping genes were amplified in order to study the relationships and comparisons of these isolates with others of the same species in NCBI database, in addition to achieve confirmation diagnosis depends on the specific genes of seven genes named with their roles arcC (Carbamate kinase), aroE(Shikimate dehydrogenase), glpF(Glycerolkinase), gmk(Guanylate ki nase), pta (Phosphate acetyltransferase), tpi (Triosephosphate isomerase) and yqi (Acetyle coenzyme A acetyltransferase).
Cite this article:
Mohmmed Lefta Atala. Bacteriological and Molecular study on S. aureus bacteria. Research J. Pharm. and Tech 2021; 14(3):1380-1384. doi: 10.5958/0974-360X.2021.00246.8