Author(s): Diah Purwaningsari, Jusak Nugraha, Sri Puji Astuti Wahyuningsih, Suhailah Hayaza, Raden Joko Kuncoroningrat Susilo, Hunsa Punnapayak, Ruey-An Doong, Win Darmanto

Email(s): windarmanto@fst.unair.ac.id

DOI: 10.5958/0974-360X.2021.00242.0   

Address: Diah Purwaningsari1,3, Jusak Nugraha3, Sri Puji Astuti Wahyuningsih2, Suhailah Hayaza2, Raden Joko Kuncoroningrat Susilo2, Hunsa Punnapayak2,4, Ruey-An Doong2,5, Win Darmanto2*
1Faculty of Medicine, University of Hang Tuah, Surabaya, Indonesia.
2Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya 60115, Indonesia.
3Department of Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
4Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
5Institute of Analytical and Environmental Sciences, National Tsing Hua University, Sec. 2 Kuang Fu Road, Hsinchu 30013, Taiwan.
*Corresponding Author

Published In:   Volume - 14,      Issue - 3,     Year - 2021


ABSTRACT:
Rheumatoid arthritis (RA) is a chronic inflammatory disease that can lead to joint destruction, deformity, and loss of function. The aim of this study is to investigate the therapeutic effect of polysaccharides krestin from Cariolus versicolor grown in Indonesia against complete Freund's adjuvant arthritis rat. Sixty-six male Wistar white rats (Rattus norvegicus) were induced by adjuvant arthritis. Samples were divided into 6 groups, 3 control (KP1, KP2, KP3) and 3 treatment groups (KP4, KP5, KP6). There were 3 different times of data collection: 1 week, 2 weeks, and 3 weeks after booster administration. Intragastric injection of polysaccharides krestin was given at a dose of 50 mg/kg body weight per day in all treatment groups. Flowcytometry blood tests were performed to examine the TGF-ß1 level and T regulator percentage. The joint scale was observed using Elisa and histopathological analysis. The analysis of various arthritic assessment parameters used in this study revealed that polysaccharides krestin from Cariolus versicolor has a considerable effect in preventing the inflammation and severe damage in adjuvant arthritis rat’s joint. The correlation between polysaccharides krestin-TGF-ß1, levels-CD4+CD25+FoxP3+Treg cells expression, and histopathological joint scale during the second weeks showed a strong correlation (p =0.05). These findings suggest that the administration of PSK from C. versicolor appears to be promising as immunomodulator and medication in chronic arthritis, therefore preclinical and clinical studies in human are highly recommended.


Cite this article:
Diah Purwaningsari, Jusak Nugraha, Sri Puji Astuti Wahyuningsih, Suhailah Hayaza, Raden Joko Kuncoroningrat Susilo, Hunsa Punnapayak, Ruey-An Doong, Win Darmanto. Immunomodulating effect of Polysaccharide Krestin from Cariolus versicolor grown in Indonesia against Rheumatoid arthritis in Rat. Research J. Pharm. and Tech. 2021; 14(3):1360-1364. doi: 10.5958/0974-360X.2021.00242.0


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