Mykola А. Komisarenko, Ivan M. Polischuk, Taras V. Upyr, Narzullos B. Saidov
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Mykola А. Komisarenko1*, Ivan M. Polischuk2, Taras V. Upyr3, Narzullos B. Saidov4
1Candidate of Pharmaceutical Sciences, Teaching Assistant, Department of Pharmacognosy, National University of Pharmacy, Pushkinska 53, Kharkiv, 61002 Ukraine.
2Postgraduate Student, Department of Chemistry of Natural Compounds and Nutritiology, National University of Pharmacy, Pushkinska 53, Kharkiv, 61002 Ukraine.
3Candidate of Pharmaceutical Sciences, Teaching Assistant, Department of Pharmacognosy, National University of Pharmacy, Pushkinska 53, Kharkiv, 61002 Ukraine.
4Doctor of Science in Pharmacy, Professor, Director of the Research Institute of the Tajik National University, Research Institute of the Tajik National University, Rudaki str. 17, 734025, Dushanbe, Tajikistan.
Volume - 14,
Issue - 3,
Year - 2021
Raspberry (Rubus idaeus L.) is a shrub up to 2m high, the fruits of which have long been used in folk medicine in the treatment of colds, influenza, as well as to restore the body after a long illness. From the fruits of raspberries harvested in Ukraine an alcohol extract was obtained. Studies of the amino acid composition of the raspberry alcohol extract were performed by HPLC on an Agilent Technologies chromatograph (model 1100). Substances were identified by the retention time of the standards. Therefore, 22 free and 21 bound amino acids were determined. The highest levels for free amino acids were found for cysteine and for glutamic acid among the bound amino acids. The immunomodulatory effect of the extract was studied in vitro at dilutions 1/200, 1/20 and 1/10 in the reaction of blast transformation of lymphocytes. Mononuclear cells (lymphocytes) were used as the test material for study activity of the raspberry extract. The lymphocytes were extracted from venous heparinized blood. The intensity of the proliferative reaction was determined. As a result, numerical data of the total number of cells and the percentage of blast forms in the samples were obtained. As a control, mitogenic stimulation of lymphocytes by phytohemagglutinin at a concentration of 2.5µg/ml was performed. Studies have shown that raspberry fruit extract has a dose-dependent immunomodulatory effect. The highest activity was shown by the extract in a dilution of 1/10, increasing the transformation activation of immunocompetent cells by 50.0 % compared to the intact control and by 10.4 % compared to the phytohemagglutinin.
Cite this article:
Mykola А. Komisarenko, Ivan M. Polischuk, Taras V. Upyr, Narzullos B. Saidov. Study of Amino Acid Composition and Immunomodulatory Activity of Rubus idaeus Alcoholic Extract. Research J. Pharm. and Tech. 2021; 14(3):1329-1332. doi: 10.5958/0974-360X.2021.00236.5
1. Zapadnyuk V.I. et al. Amino acids in medicine. Edited by Zdorov’ya, Kiev. 1982; pp. 200. (in Russian)
2. Lastukhin Yu. O. Chemistry of natural organic compounds: a textbook. Edited by Lviv Polytechnic National University, “Intellect-West”, Lviv. 2005; pp 560. (in Ukrainian)
3. Dawson R. et al. Handbook of Biochemist: translated from English. Edited by Mir, Moscow. 1991; pp 544. (in Russian)
4. Colman J. Visual Biochemistry: translated from German. Edited by Mir, Moscow. 2000. pp 469. (in Russian)
5. Mashkovsky M.D. Medicines: in 2 vol. Edited by Medicine, Moscow. 2003; Vol.1. pp 539; Vol.2. pp.608. (in Russian)
6. Murray R, Grenner D. Human biochemistry: a manual in 2 vol. Edited by Mir, Moscow., 1993. Vol.1., pp. 384; Vol.2., pp. 415. (in Russian)
7. Kretovich V.L. Fundamentals of Plant Biochemistry. Edited by Vysshaya Shkola, Moscow. 1980; pp. 503. (in Russian)
8. Barnaulov O. D. Phytotherapy of patients with bronchopulmonary diseases. Saint Petersburg: Publisher N-L. 2008; pp. 304. (in Russian).
9. Salem M. L. Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. Intern. Immunopharmacol. 2005; 5: 1749–1770.
10. A. Jámbor, I. Molnár-Perl. Quantitation of amino acids in plasma by high performance liquid chromatography: Simultaneous deproteinization and derivatization with 9-ﬂuorenylmethyloxycarbonyl chloride. Journal of Chromatography A. 2009; 1216: 6218–6223.
11. A. Jámbor, I. Molnár-Perl. Amino acid analysis by high-performance liquid chromatography after derivatization with 9-ﬂuorenylmethyloxycarbonyl chloride. Literature overview and further study. Journal of Chromatography A, 2009,1216: 3064–3077.
12. Kiseleva E.P., Zweibakh A.S., Goldman E.I. et al. Using a micromethod for blast transformation of human and animal lymphocytes. Immunology. 1985; 1: 76–78. (in Russian).
13. Nikitin V.M. Immunology Method Reference. Edited by Štiinca, Kishinev. 1982; pp. 304. (in Russian).
14. Frolov V. M., Peresadin N. A., Pshenichny I. Ya. Determination of the phagocytic activity of monocytes of peripheral blood in patients. Laboratory profession. 1990; 9: 27–29. (in Russian).
15. Kvatchoff V. G. Application de subculture de leycocytes en test-systeme devalution immunomodule de vims in vitro. Abstracts of IV International Congress Cell Biology. Montreal (Canada) 1988; P. 205.
16. Garnik T.P., Frolov V.M., Peresadin N. A. Efficacy of herbal drugs deprivation and immunoplus in the treatment of patients with chronic fatigue syndrome. Ukrainian Medical Almanac. 2008; 11 (5): 50-56. (in Ukrainian).
17. Movafagh A., Heydary Н., Mortazavi-Tabatabaei S. A., Azargashb E. The Significance Application of Indigenous Phytohemagglutinin (PHA) Mitogen on Metaphase and Cell Culture Procedure. Iran. J. Pharm. Res. 2011; 10 (4): 895-903
18. Lapach S. N., Chubenko A. V., Babich P. N. Statistical methods in biomedical research using Excel. Киев: Edited by Morion 2000, Kiev; pp. 320. (in Russian).