Nguyen Pham Quynh Anh, Tran Thi Hai Yen, Doan Thi Thanh Vinh, Tran Phu Tien, Nguyen Anh Dung, Nguyen Hoang Khue Tu
Nguyen Pham Quynh Anh1,2, Tran Thi Hai Yen1,2, Doan Thi Thanh Vinh1,2, Tran Phu Tien1,2, Nguyen Anh Dung1,2,3, Nguyen Hoang Khue Tu1,2*
1School of Biotechnology, International University, Vietnam.
2Vietnam National University – Ho Chi Minh City, Quarter 6, Linh Trung ward, Thu Duc district, Vietnam.
3Faculty of Nautral Sciences, Thu Dau Mot University.
Volume - 14,
Issue - 3,
Year - 2021
The most abundant isomers of conjugated linoleic acid (CLA) are cis-9 trans-11 (c9t11) and trans-10 cis-12 (t10c12) that involved in a number of health aspect. At present, lactic acid bacteria was thought to produce CLA from LA. Therefore, the study was conducted to determine the conjugated linoleic acid (CLA) producing ability of Lactobacillus fermentum A01 (L. fermentum A01) in grape seed oil and linoleic acid. The CLA was detected on thin layer chromatography and gas chromatography – mass spectrum (GC-MS) analysis after converting to fatty acid methyl esters. L. fermentum A01 could produce CLA by thin layer chromatography (TLC) analysis. Further investigation revealed L. fermentum A01 cultured in medium containing linoleic acid (MRS-LA) for 36 h could produce 9,11 di-ene of C18 at the retention time of 30.299 min with identical percentage (0.67%). Moreover, there were many products produced as 9, 12; 9, 15; 11; 10; 14-C18 with higher percentage in MRS-LINO and MRS-Oil. This study was the preliminary report of L. fermentum A01 in the ability of converting LA to CLA.
Cite this article:
Nguyen Pham Quynh Anh, Tran Thi Hai Yen, Doan Thi Thanh Vinh, Tran Phu Tien, Nguyen Anh Dung, Nguyen Hoang Khue Tu. Study on the ability of producing of conjugated Linoleic acid of Lactobacillus fermentum A01 isolated from human digestive tract. Research J. Pharm. and Tech 2021; 14(3):1319-1322. doi: 10.5958/0974-360X.2021.00234.1
1. Rashad R, Salem AH and Salem AEG. Production of free conjugated linoleic acid by fermentation performed using Lactobacillus casei and Bifidobacterium bifidum. Global Veterinaria. 2015;4(5):720-728.
2. Christie WW, Dobson G and Gunstone FD. Isomers in commercial samples of conjugated linoleic acid. Lipids. 1997;32(11):1231.doi: 10.1007/s11745-997-0158-1
3. Bhattacharya A, Banu J, Rahman M, Causey J and Fernandes G. Biological effects of conjugated linoleic acids in health and disease. Journal of Nutritional Biochemistry. 2006;17(12):789-810.
4. Mahmoud AS, Elsanhoty RM and Elhadary AE. The impact of oil type and lactic acid bacteria on conjugated linoleic acid production. Journal of Biochemistry, Microbiology and Biotechnology. 2016;4(2):25-29.
5. De Man J, Rogosa M and Sharpe M. A medium for the cultivation of lactobacilli. Journal of Applied Bacteriology. 1960;23(1):130-135.
6. Metcalfe L, Schmitz A and Pelka J. Rapid Preparation of Fatty Acid Esters from Lipids for Gas Chromatographic Analysis. Analytical Chemistry. 1966;38(3):514-515.
7. Kathryn EY, Sarah MQ and Stephen JT. Comparing Gas Chromatographic Techniques Used in Fatty Acid Profiling of Northern Fur Seals (Callorhinusursinus) and Steller Sea Lions (Eumetopiasjubatus) from Lovushki Island Complex, Russia. International Journal of Applied Science and Technology. 2012; 2(9):11-21.
8. Bligh E and Dyer W. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology.1959;37(1):911-917.
9. Barber ED. Identification of aromatic compounds as simple azines by paper and thin-layer chromatography. Journal of Chromatography. 1967;27(2):398-406.