Author(s): Afiat Berbudi, Nur Rahmi, Nur Atik, Tenny Wikayani, Nurul Qomarilla, Nurul Setia Rahayu, Almahitta Cintami Putri


DOI: 10.5958/0974-360X.2021.00194.3   

Address: Afiat Berbudi1,2*, Nur Rahmi3*, Nur Atik4, Tenny Wikayani5, Nurul Qomarilla5, Nurul Setia Rahayu6, Almahitta Cintami Putri7
1Department of Biomedical Sciences, Parasitology Division, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
2Faculty of Medicine, Universitas Pasundan, Bandung, West Java, Indonesia.
3 Undergraduate Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia.
4Department of Biomedical Sciences, Cell Biology Division, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
5Cell Culture and Cytogenetic Laboratory, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
6Molecular Genetic Laboratory, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
7Department of Surgery, Plastic and Reconstructive Surgery Division, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital, Bandung, Indonesia. *Corresponding Author

Published In:   Volume - 14,      Issue - 2,     Year - 2021

Curcuma longa (turmeric) has been widely used to accelerate wound healing, but the underlying mechanism remains unclear. Wound healing consists of four phases which are correlated and overlapping, i.e., coagulation, inflammation, proliferation and remodeling. Macrophages play an important role in most phases. Macrophage polarization to M2 initiates the proliferation phase, which is characterized by the production of several crucial growth factors. Since turmeric has been known to be an herb that accelerates wound closure, we investigated whether Curcuma longa extract administration in macrophage culture induces M2 macrophage switching. An in vitro study was performed using peritoneal macrophages from Swiss Webster strain mice. Peritoneal cells were collected and cultured in a 24-well plate. After 2 hours of incubation, macrophages (adherent cells) were treated with 0.5 ppm, 1.0 ppm and 5.0 ppm of ethanolic extract of Curcuma longa and incubated for 2 days. Quantitative real time PCR was performed to quantify M2 and M1 marker gene expression. The results revealed the upregulation of M2 marker (Arginase-1) expression upon administration of 0.5 ppm of Curcuma longa extract, but not of higher doses (1.0 and 5.0 ppm). In parallel, the ratio of Arg-1/Inos was high upon administration of 0. 5ppm of extract. In conclusion, Curcuma longa extract induces in vitro M2 polarization in low-dose administration.

Cite this article:
Afiat Berbudi, Nur Rahmi, Nur Atik, Tenny Wikayani, Nurul Qomarilla, Nurul Setia Rahayu, Almahitta Cintami Putri. The administration of low-dose Curcuma longa extract induces M2 polarization in peritoneal macrophage culture. Research J. Pharm. and Tech. 2021; 14(2):1079-1084. doi: 10.5958/0974-360X.2021.00194.3

1.    Sen CK, Gordillo GM, Roy S, Kirsner R, Lambert L, Hunt TK, et al. Human skin wounds: a major and snowballing threat to public health and the economy. Wound Repair Regen. 2009;17(6): 763-71.
2.    Graves N, Zheng H. The prevalence and incidence of chronic wounds: a literature review. Wound Pract Res. 2014;22(1): 4-19.
3.    Demidova-Rice TN, Hamblin MR HI. Acute and impaired wound healing: Pathophysiology and current methods for drug delivery, part 1: Normal and chronic wounds: Biology, causes, and approaches to care. Adv Ski Wound Care. 2012;25(7): 304–14.
4.    Järbrink K, Ni G, Sönnergren H, Schmidtchen A, Pang C, Bajpai R, et al. Prevalence and incidence of chronic wounds and related complications: a protocol for a systematic review. Syst Rev. 2016;5(1): 152.
5.    Campbell L, Saville CR, Murray PJ, Cruickshank SM, Hardman MJ. Local Arginase 1 Activity Is Required for Cutaneous Wound Healing. J Invest Dermatol. 2013;133(10): 2461-70.
6.    Pawar R, Toppo F, Mandloi A, Shaikh S. Exploring the role of curcumin containing ethanolic extract obtained from Curcuma longa (rhizomes) against retardation of wound healing process by aspirin. Indian J Pharmacol. 2015;47(2): 160.
7.    Akbik D, Ghadiri M, Chrzanowski W, Rohanizadeh R. Curcumin as a wound healing agent. Life Sci. 2014;116(1):1-7.
8.    Ferrante CJ, Leibovich SJ. Regulation of Macrophage Polarization and Wound Healing. Adv Wound Care. 2011;1: 10-6.
9.    Zhou Y, Zhang T, Wang X, Wei X, Chen Y, Guo L, et al. Curcumin Modulates Macrophage Polarization Through the Inhibition of the Toll-Like Receptor 4 Expression and its Signaling Pathways. Cell Physiol Biochem. 2015;36(2): 631-41.
10.    Ikpeama, Ahamefula, Onwuka GI, Nwankwo, Chibuzo. Nutritional Composition of Tumeric (Curcuma longa) and its Antimicrobial Properties. Int J Sci Eng Res. 2014;5(10): 1085-9.
11.    Ray A, Dittel BN. Isolation of mouse peritoneal cavity cells. J Vis Exp. 2010;(35).
12.    Zhang X, Goncalves R, Mosser DM. The isolation and characterization of murine macrophages. Current Protocols in Immunology. 2008.
13.    Karahashi H, Amano F. Lipopolysaccharide (LPS)-induced cell death of C3H mouse peritoneal macrophages in the presence of cycloheximide: different susceptibilities of C3H/HeN and C3H/HeJ mice macrophages. J Endotoxin Res. 2000;6(1):33-9.
14.    Shiyou L, Wei Y, Guangrui D, Ping W, Peiying Y, Bharat A. Chemical composition and product quality control of turmeric (Curcuma longa L.). Pharm Crop. 2011;2: 28-54.
15.    Tu SP, Jin H, Shi JD, Zhu LM, Suo Y, Lu G, et al. Curcumin Induces the Differentiation of Myeloid-Derived Suppressor Cells and Inhibits Their Interaction with Cancer Cells and Related Tumor Growth. Cancer Prev Res. 2012;5(2):205–15.
16.    Gao S, Zhou J, Liu N, Wang L, Gao Q, Wu Y, et al. Curcumin induces M2 macrophage polarization by secretion IL-4 and/or IL-13. J Mol Cell Cardiol. 2015;85: 131-9.
17.    Chen F, Guo N, Cao G, Zhou J, Yuan Z. Molecular analysis of curcumin-induced polarization of murine RAW264.7 macrophages. J Cardiovasc Pharmacol. 2014;63(6): 544-52.
18.    Martinez FO, Sica A, Mantovani A, Locati M. Macrophage activation and polarization. Bioscience. 2008;13(4): 453-61.
19.    Junttila IS, Mizukami K, Dickensheets H, Meier-Schellersheim M YH, Donnelly RP et al. Tuning sensitivity to IL-4 and IL-13: differential expression of IL-4R alpha, IL-13R alpha1, and gammac regulates relative cytokine sensitivity. J Exp Med. 2008;205(11): 2595–608.
20.    Lucas T, Waisman A, Ranjan R, Roes J, Krieg T, Muller W, et al. Differential Roles of Macrophages in Diverse Phases of Skin Repair. J Immunol. 2010;184(7): 3964–77.
21.    Daley JM, Brancato SK, Thomay AA, Reichner JS, Albina JE. The phenotype of murine wound macrophages. J Leukoc Biol. 2010;87(1): 59-67.
22.    Fraternale A, Brundu S, Magnani M. Polarization and Repolarization of Macrophages. J Clin Cell Immunol. 2015;6(2): 1-10.
23.    Kundu S, Biswas TK, Das P, Kumar S, De DK. Turmeric (Curcuma longa) Rhizome Paste and Honey Show Similar Wound Healing Potential: A Preclinical Study in Rabbits. Int J Low Extrem Wounds. 2005 Dec 29;4(4): 205-13.
24.    Janeway CA, Medzhitov R. Innate Immune Recognition. Annu Rev Immunol. 2002;20(1): 197-216.
25.    Mukhopadhyay S, Peiser L, Gordon S. Activation of murine macrophages by Neisseria meningitidis and IFN-γ in vitro: distinct roles of class A scavenger and Toll-like pattern recognition receptors in selective modulation of surface phenotype. J Leukoc Biol.;76(3): 577-84.
26.    Erbel C, Rupp G, Helmes CM, Tyka M, Linden F, Doesch AO, et al. An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization. J Vis Exp. 2013;76(June): 1-8.
27.    Jaguin M, Houlbert N, Fardel O, Lecureur V. Polarization profiles of human M-CSF-generated macrophages and comparison of M1-markers in classically activated macrophages from GM-CSF and M-CSF origin. Cell Immunol. 2013;281(1): 51-61.

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