Author(s): Golamari Siva Reddy, Mallu Maheswara Reddy, Nadeem Siddiqui, Varakala Nikhil Reddy, Neeha Sultana, Divyanshu Dhakate, Ravavarapu Sai Tripura, N Konda Reddy

Email(s): gsiva@kluniversity.in

DOI: 10.5958/0974-360X.2021.00171.2   

Address: Golamari Siva Reddy1*, Mallu Maheswara Reddy1, Nadeem Siddiqui1, Varakala Nikhil Reddy1, Neeha Sultana1, Divyanshu Dhakate1, Ravavarapu Sai Tripura1, N Konda Reddy1
1Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, AP, India.
1Department of Mathematics, Koneru Lakshmaiah Education Foundation, Vaddeswaram, AP, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 2,     Year - 2021


ABSTRACT:
Bio surfactants are used to reduce the surface tension between molecules and also plays a major role in microbial enhanced oil recovery and potential uses in territories, which include environmental remediation, agriculture, bio film formation, quorum sensing, textile, prescribed drugs, cosmetics, and the food, oil, and petrochemical industries. In the present paper, optimization of medium components for biosurfactant production by Achromobacter xylos GSR-21 using statistical experimental design was studied. The mineral salt medium requirement for biosurfactant production by Glycolipid Achromobacter xylos was optimized. The important medium components, identified by the initial screening method of Plackett-Burman and response surface quadratic methodology was applied to further optimize bio surfactant production. Regression coefficients were calculated by regression analysis and the model equation was determined. R2 value for biosurfactant (g/L) was calculated as 0.88, and it indicates that the model was well fitted with the experimental results. The important medium components, identified by the initial screening method of Plackett-Burman, were KH2PO4, NH4NO3 and Glycerol (g/l). Response surface quadratic methodology was applied to further optimize biosurfactant production. The maximum biosurfactant production (Achromobacter xylos) (17.9g/L) was predicted at the optimized values of KH2PO4-20 (g/l), glycerol-30 (g/l) and NH4NO3-20(g/l). Surface plots were made, and the obtained mathematical model was verified by performing the experiment with the predicted optimized values, and the yield of bio-surfactant was found to be 9.89g/L. Validation of the predicted model was fitted 98.9% with the experimental results conducted at the optimum conditions. Results of this statistical analysis showed that KH2PO4, glycerol and NH4NO3 had found significant medium components for biosurfactant (Achromobacter xylos) production.


Cite this article:
Golamari Siva Reddy, Mallu Maheswara Reddy, Nadeem Siddiqui, Varakala Nikhil Reddy, Neeha Sultana, Divyanshu Dhakate, Ravavarapu Sai Tripura, N Konda Reddy. Role of Medium Components for the Production of Biosurfactant by Achromobacter xylos GSR-21. Research J. Pharm. and Tech. 2021; 14(2):959-965. doi: 10.5958/0974-360X.2021.00171.2


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