Golamari Siva Reddy, Mallu Maheswara Reddy, Nadeem Siddiqui, Varakala Nikhil Reddy, Neeha Sultana, Divyanshu Dhakate, Ravavarapu Sai Tripura, N Konda Reddy
Golamari Siva Reddy1*, Mallu Maheswara Reddy1, Nadeem Siddiqui1, Varakala Nikhil Reddy1, Neeha Sultana1, Divyanshu Dhakate1, Ravavarapu Sai Tripura1, N Konda Reddy1
1Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, AP, India.
1Department of Mathematics, Koneru Lakshmaiah Education Foundation, Vaddeswaram, AP, India.
Volume - 14,
Issue - 2,
Year - 2021
Bio surfactants are used to reduce the surface tension between molecules and also plays a major role in microbial enhanced oil recovery and potential uses in territories, which include environmental remediation, agriculture, bio film formation, quorum sensing, textile, prescribed drugs, cosmetics, and the food, oil, and petrochemical industries. In the present paper, optimization of medium components for biosurfactant production by Achromobacter xylos GSR-21 using statistical experimental design was studied. The mineral salt medium requirement for biosurfactant production by Glycolipid Achromobacter xylos was optimized. The important medium components, identified by the initial screening method of Plackett-Burman and response surface quadratic methodology was applied to further optimize bio surfactant production. Regression coefficients were calculated by regression analysis and the model equation was determined. R2 value for biosurfactant (g/L) was calculated as 0.88, and it indicates that the model was well fitted with the experimental results. The important medium components, identified by the initial screening method of Plackett-Burman, were KH2PO4, NH4NO3 and Glycerol (g/l). Response surface quadratic methodology was applied to further optimize biosurfactant production. The maximum biosurfactant production (Achromobacter xylos) (17.9g/L) was predicted at the optimized values of KH2PO4-20 (g/l), glycerol-30 (g/l) and NH4NO3-20(g/l). Surface plots were made, and the obtained mathematical model was verified by performing the experiment with the predicted optimized values, and the yield of bio-surfactant was found to be 9.89g/L. Validation of the predicted model was fitted 98.9% with the experimental results conducted at the optimum conditions. Results of this statistical analysis showed that KH2PO4, glycerol and NH4NO3 had found significant medium components for biosurfactant (Achromobacter xylos) production.
Cite this article:
Golamari Siva Reddy, Mallu Maheswara Reddy, Nadeem Siddiqui, Varakala Nikhil Reddy, Neeha Sultana, Divyanshu Dhakate, Ravavarapu Sai Tripura, N Konda Reddy. Role of Medium Components for the Production of Biosurfactant by Achromobacter xylos GSR-21. Research J. Pharm. and Tech. 2021; 14(2):959-965. doi: 10.5958/0974-360X.2021.00171.2
1. Kumar AP, Janardhan A, Radha S, Viswanath B, Narasimha G. Statistical approach to optimize production of biosurfactant by Pseudomonas aeruginosa 2297. 3 Biotech. 2015 Feb 1;5(1):71-9.
2. Reddy RS. Statistical optimization of medium components for biosurfactant production by Achromobacter xylos GSR21. International Journal of Green Pharmacy (IJGP). 2019 Feb 5;12(04).
3. Diniz Rufino R, Moura de Luna J, de Campos Takaki GM, Asfora Sarubbo L. Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988. Electronic Journal of Biotechnology. 2014 Jan;17(1)
4. Wei YH, Lai HC, Chen SY, Yeh MS, Chang JS. Biosurfactant production by Serratia marcescens SS-1 and its isogenic strain SMΔR defective in Spn R, a quorum-sensing Lux R family protein. Biotechnology letters. 2004 May 1;26(10):799-802.
5. Perfumo A, Rancich I, Banat IM. Possibilities and challenges for biosurfactants use in petroleum industry. In Biosurfactants 2010 (pp. 135-145). Springer, New York, NY.
6. Lotfy WA, Ghanem KM, El-Helow ER. Citric acid production by a novel Aspergillus niger isolate: II. Optimization of process parameters through statistical experimental designs. Bioresource Technology. 2007 Dec 1;98(18):3470-7.
7. Tanyildizi MS, Özer D, Elibol M. Optimization of α-amylase production by Bacillus sp. using response surface methodology. Process Biochemistry. 2005 Jun 1;40(7):2291-6.
8. Reddy GS, Srinivasulu K, Mahendran B, Reddy RS. Production and stability studies of the biosurfactant isolated from Achromobacter xylos GSR-21. Biointerface Research in Applied Chemistry. 2018 Aug 15;8(4):3388-94.
9. Plackett RL, Burman JP. The design of optimum multifactorial experiments. Biometrika. 1946 Jun 1;33(4):305-25.
10. Box GE. Multi-factor designs of first order. Biometrika. 1952 May 1;39(1-2):49-57.
11. Montgomery DC. Response surface methods and other approaches to process optimization. Design and analysis of experiments. 1997.
12. Al-Araji LI, Rahman RN, Basri M, Salleh AB. Optimisation of rhamnolipids produced by Pseudomonas aeruginosa 181 using Response Surface Modeling. Annals of microbiology. 2007 Dec 1;57(4):571.
13. Reddy, Golamari Siva, Botlagunta Mahendran, and Ronda Srinivasa Reddy. "Screening and Optimization of Achromobacter xylosoxidans GSMSR13B Producing Bacteria." Asian Journal of Chemistry 30, no. 7 (2018).
14. Cunha CD, Do Rosario M, Rosado AS, Leite SG. Serratia sp. SVGG16: a promising biosurfactant producer isolated from tropical soil during growth with ethanol-blended gasoline. Process Biochemistry. 2004 Oct 29;39(12):2277-82.
15. Singh A, Van Hamme JD, Ward OP. Surfactants in microbiology and biotechnology: Part 2. Application aspects. Biotechnology advances. 2007 Jan 1;25(1):99-121.
16. Yin H, Qiang J, Jia Y, Ye J, Peng H, Qin H, Zhang N, He B. Characteristics of biosurfactant produced by Pseudomonas aeruginosa S6 isolated from oil-containing wastewater. Process Biochemistry. 2009 Mar 1;44(3):302-8.
17. Reddy GS, Mahendran B, Reddy RS. Kinetic Measurements for Achromobacter xylos GSR-21 During Biosurfactant Production in Two-phase system and developing a Double-exponential model for viable cell profile . Journal of Pharmaceutical Sciences and Research. 2018 May 1;10(5):1223-8.
18. Bento FM, Beech IB, Gaylarde CC, Englert GE, Muller IL. Degradation and corrosive activities of fungi in a diesel–mild steel–aqueous system. World Journal of Microbiology and Biotechnology. 2005 Mar 1;21(2):135-42.
19. Md F. Biosurfactant: production and application. J Pet Environ Biotechnol. 2012;3(4):124.
20. Makkar RS, Rockne KJ. Comparison of synthetic surfactants and biosurfactants in enhancing biodegradation of polycyclic aromatic hydrocarbons. Environmental Toxicology and Chemistry: An International Journal. 2003 Oct;22(10):2280-92.
21. Banat IM, Franzetti A, Gandolfi I, Bestetti G, Martinotti MG, Fracchia L, Smyth TJ, Marchant R. Microorganism in environmental management: microbes and environment. Appl Microbiol Biotechnol. 2010; 87: 427-44.
22. Buchanan RE, Buchanan RE, Gibbons NE. Bergey's Manual of Determinative Bacteriology. William's and Wilkins Company; 1970.
23. Chikere CB, Obieze CC, Okerentugba P. Molecular assessment of microbial species involved in the biodegradation of crude oil in saline Niger Delta sediments using bioreactors. Journal of Bioremediation & Biodegradation. 2015 Aug 7;6(5):1-7.
24. Das R, Tiwary BN. Isolation of a novel strain of Planomicrobium chinense from diesel contaminated soil of tropical environment. Journal of Basic Microbiology. 2013 Sep;53(9):723-32.
25. Komal K, Anuradha P, Aruna K. Studies on biosurfactant production by Pseudomonas aeruginosa R2 isolated from oil contaminated soil sample. Asian Journal of Bio Science. 2012;7(2):123-9.
26. Kumar SS, Sharmila G, Muthukumaran C, Tamilarasan K, Gopinath M. Statistical optimization of critical medium components for biosurfactant production by Bacillus subtilis. Journal of BioScience & Biotechnology. 2015 May 1;4(2).
27. Reddy, Golamari Siva, Kamma Srinivasulu, Botlagunta Mahendran, and Ronda Srinivasa Reddy. "Biochemical Characterization of Anti-Microbial Activity and Purification of Glycolipids Produced by Dodecanoic Acid-Undecyl Ester." Research Journal of Pharmacy and Technology 11, no. 9 (2018): 4066-4073.
28. Reddy, Golamari Siva, S. Pranavi, B. Srimoukthika, and Vuyyuru Viswanadh Reddy. "Isolation and Characterization of Bacteria from Compost for Municipal Solid Waste from Guntur and Vijayawada." Journal of Pharmaceutical Sciences and Research 9, no. 9 (2017): 1490.