Renuka R, Jeyanthi G.P
Renuka R1, Jeyanthi G.P2
1Department of Biochemistry, Sri Ramakrishna College of Arts and Science for Women, Coimbatore, Tamil Nadu, India.
2Research and Consultancy, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, Tamil Nadu, India.
Volume - 14,
Issue - 12,
Year - 2021
Momordica charantia L., which belongs to the Cucurbitaceae family is also known as bitter gourd, bitter melon, karela and paharkai in Tamil has been widely used as a vegetable and also as herbal medicine for diabetic patients. Identification of bioactive compounds in the plant material may be useful for proper standardization of herbs and their formulations. The present study was conducted to analyze the antidiabetic compounds present in Momordica charantia seed extracts using FTIR (Fourier Transform Infra-Red spectrophotometry) and GCMS (Gas Chromatography Mass Spectroscopy) analysis. FT-IR analysis of ethyl acetate seed extracts of Momordica charantia confirmed the presence of alcohols, phenols, alkanes, alkynes, aldehydes, aromatic compounds, aromatic amines and aromatic hydrocarbons. GC-MS analysis of ethyl acetate seed extracts of Momordica charantia revealed the presence of about forty compounds in that four were found to be biologically important as per literature studies because of their anti diabetic, antioxidant, antimicrobial and anticancer properties.
Cite this article:
Renuka R, Jeyanthi G.P. FTIR and GCMS Analysis of Antidiabetic Compounds in Ethyl acetate Seed Extracts of Momordica charantia. Research Journal of Pharmacy and Technology. 2021; 14(12):6705-9. doi: 10.52711/0974-360X.2021.01158
Renuka R, Jeyanthi G.P. FTIR and GCMS Analysis of Antidiabetic Compounds in Ethyl acetate Seed Extracts of Momordica charantia. Research Journal of Pharmacy and Technology. 2021; 14(12):6705-9. doi: 10.52711/0974-360X.2021.01158 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-12-88
1. Dharmesh Sharma, Deepak Prashar, Sanjay Saklani. Bird’s Eye View on Herbal Treatment of Diabetes. Asian J. Pharm. Res. Jan.-Mar. 2012; 2(1): Page 01-06.
2. Krishna Ravi, Rosmi Jose, Sumitha SK, Teena Johny, Krishnaveni K, Shanmuga Sundaram R, Sambath Kumar R. An Overview of Treatment Challenges and the role of Herbal Antioxidants in Diabetes Mellitus. Research J. Pharm. and Tech. 2017; 10(8): Page 2765-2770.
3. Prakash, O., Rajesh, K., Ritika, S., Pragya, T., Shradha, M and Ajeet. Plants explored with antidiabetic properties: a review, American Journal of Pharmacological Sciences. 2015; 3(3): Page 55-66.
4. Nikhat F., Satyanarayana D.,Shastri C.S., Moid A., Thouheed A.. Investigation of Phytoconstituents, from Momordica charantia Linn Roots and Screening them, for Anti-Hyperglycemic Activity. Asian J. Research Chem. March 2014; 7(3): Page 256-26.
5. Saifi, A., Namdeo, K. P., Bodakhe, S.H and Dwedi, JA. review on antidiabetic potential of Momordica charantia Linn, International Journal of Pharmaceutical Research and Bio-Science.2013; 2,(6): Page 475-485.
6. Nikhat F., Satyanarayana D.,Shastri C.S., Moid A., Thouheed A.. Investigation of Phytoconstituents, from Momordica charantia Linn Roots and Screening them, for Anti-Hyperglycemic Activity. Asian J. Research Chem. March 2014; 7(3): Page 256-261.
7. Bahare Salehi, Athar Ata,Nanjangud V. Anil Kumar,et.al. Antidiabetic Potential of Medicinal Plants and Their Active Components. 2019; 9 (10): Page 551.
8. Immanuel Selvaraj C and Vijayalakshmi K.. Medicinal plants and their phyto-constituents with potential antidiabetic activity - A review Research Jpurnal of Biotechnology. 2018; 13(5): Page 92-99.
9. A. Rajendran, R. Sudeshraj, S. Sureshkumar Potential antidiabetic activity of medicinal plants – A short review, The Journal of Phytopharmacology 2018; 7(5): Page 456-459
10. P. Jaya Preethi. Herbal Medicine for Diabetes Mellitus: A Review. Asian J. Pharm. Res. April- June 2013; 3(2): Page 57-70
11. Mohd. Yaqub Khan, Irfan Aziz, Bipin Bihari, Hemant Kumar, Maryada Roy, Vikas Kumar Verma. A Review- Phytomedicines Used in Treatment of Diabetes. Asian J. Pharm. Res. July-Sept. 2014; 4(3):Page 135-154.
12. Krishnananda P Ingle, Amit G Deshmukh, Dipika A Padole, Mahendra S Dudhare, Mangesh P Moharil and Vaibhav C. Khelurkar, Phytochemicals: Extraction methods, identification and detection of bioactive compounds from plant extracts Journal of Pharmacognosy and Phytochemistry 2017; 6(1): Page 32-36
13. Fried, B and Sherma, J. Thin-layer chromatography: Techniques and applications, Chromatographic Science Series, 66, Marcel Dekker, Inc. New York, USA, 1994; (3): Page 451.
14. Murugan, K., Geethu, M.G., Suchithra, P., Kavitha. C.H., Aswathy, J.M and Dinesh, B. Fourier Transform Infrared Spectroscopy analysis of different solvent extracts of water hyacinth (Eichhornia crassipes Mart Solms) an allelopathic approach, World Journal of Pharmacy and Pharmaceutical Sciences.2014; 3(6): Page 256-1266.
15. Rajat Rana, Ajay Kumar, Rohit Bhatia. Impact of Infra Red Spectroscopy in Quantitative Estimation: An Update. Asian J. Pharm. Ana. 2020; 10(4): Page 218-230.
16. Mercy, J., Latha, K and Vanaja, R. Determination of bioactive components of decholestrate, a polyherbal formulation by GC-MS Analysis, New York Science Journal. 2013; 6 (5): Page 1-5.
17. Mahesha,M.P., Vishnumurthy, K.A and Vasudeva, A.A. Extraction,characterization and biological studies of phytochemicals from Mammea suriga , Journal of Pharmaceutical Analysis. 2015, 5 (3): Page 182–189.
18. Ragavendran, P., Sophia, D., Arul, R.C and Gopalakrishnan,V.K. Functional group analysis of various extracts of Aerva lanata (L) by FTIR spectrum, Pharmacology Online. 2011; 1(4): Page 358- 364.
19. C. Yogeshwari, P. Kumudha. Spectroscopic Analysis of Bioactive Components of Ichnocarpus frutescens R. Br. Res. J. Pharmacognosy and Phytochem. 2018; 10(2): Page 171-174.
20. Murali, R., Arumugam, K and Ramalingam, S. Protective effects of D-limonene on lipid peroxidation and antioxidant enzymes in streptozotocin-induced diabetic rats, Basic and Clinical Pharmacology and Toxicology, 2013; 112, Page 175–181.
21. Jadhav Sameer S., Salunkhe Vijay R. , Magdum Chandrakant S.. Daily Consumption of Antioxidants:-Prevention of Disease is better than Cure. Asian J. Pharm. Res. Jan.-Mar. 2013; 3(1): Page 33-39.
22. Parul,S and Rekha,V, In vitro α-amylase inhibitory activity and GC-MS analysis of Petrea volubilis, International Journal of Science and Research.2015; 4 (4): Page 190-194.
23. Deepthy, M.M.J and Radhamany, P.M. GC- MS profiling in the fruits of Tamilnadia Uliginosa (Retz.) Tirveng and Sastre (Rubiaceae), World Journal of Pharmacy and Pharmaceutical Sciences. 2016; 5(1): Page 890-896.
24. Genta,S.B., Cabrera,W.M., Mercado,M., Grau,A., Catalan,C.A and Sanchez,S.S. Hypoglycemic activity of leaf organic extracts from Smallanthus sonchifolius: Constituents of the most active fractions, Chemico Biological Interactions.2010; 185 (2): Page 143-52.
25. Renuka R. and Jeyanthi G. P, Evaluation of in vitro α- amylase inhibitory kinetics and free radical scavenging activities of Momordica charantia International Journal of ChemTech Research. 2017; 10 (7): 315-323.
26. Ujjwala Supe, Prarthna Daniel. HPLC Method for Analysis of Bioactive Compound from Momordica charantia. Research J. Pharm. and Tech. Dec 2015; 8(12): Page 1649-1652.
27. Renuka R. and Jeyanthi G. P,TLC, HPTLC Finger Print analysis and antidiabetic evaluation of Momordica charantia and Trigonella foenum graecum seed extracts, International Journal of Recent Scientific Research. Dec 2019; 10, 12(C): Page 36418-36421.
28. Sachin U Rakesh, Priyanka R Patil , Vijay R Salunkhe. Momordica Charantia Linn – A Comprehensive Review. Research J. Pharmacognosy and Phytochemistry 2009; 1(2): Page 98-102.