Author(s): Nawan, Septi. Handayani

Email(s): nawan@med.upr.ac.id , dr_na1tanan@yahoo.com

DOI: 10.52711/0974-360X.2021.01147   

Address: Nawan1*, Septi. Handayani2
1Department of Microbiology, Faculty of Medicine, Palangka Raya University, Jl. H. Timang Kompleks Tunjung Nyaho 73111, Palangka Raya, Indonesia.
2Department of Biochemistry, Faculty of Medicine, Palangka Raya University, Jl. H. Timang Kompleks Tunjung Nyaho 73111, Palangka Raya, Indonesia.
*Corresponding Author

Published In:   Volume - 14,      Issue - 12,     Year - 2021


ABSTRACT:
Bacterial identification with 16S rRNA accompanied with cloning techniques to obtain a purer sequence are now highly developed. This research aims to identify Streptomyces bacteria from peatlands in Central Kalimantan as a source of new antibiotics compound using 16srRNA and cloning techniques. Streptomyces sp. isolate was studied in detail and characterized. The morphological and biochemical characterization and tentative identification methods was described following the Bergey’s Manual of Systematic Bacteriology. Further identification and phylogenetic study were done using molecular tools. 16S rRNA helped to determine the species using PCR. Results of tabulation data from BLAST® indicate that isolates 2.1 belong to the genus Streptomyces sp. with the percentage of identity reaching 99.73% with Streptomyces sp. VEL 17 gene for 16S rRNA, partial sequences.


Cite this article:
Nawan, Septi. Handayani. Molecular identification of Streptomyces sp. isolated from peat land of Palangka Raya, Kalimantan Tengah using 16S rRNA gene sequences analysis. Research Journal of Pharmacy and Technology. 2021; 14(12):6639-4. doi: 10.52711/0974-360X.2021.01147

Cite(Electronic):
Nawan, Septi. Handayani. Molecular identification of Streptomyces sp. isolated from peat land of Palangka Raya, Kalimantan Tengah using 16S rRNA gene sequences analysis. Research Journal of Pharmacy and Technology. 2021; 14(12):6639-4. doi: 10.52711/0974-360X.2021.01147   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-12-77


REFERENCES:
1.     Sreeja MK, Gowrishankar NL, Adisha S, Divya KC. Antibiotic resistance-reasons and the most common resistant pathogens - A review. Res J Pharm Technol. 2017;10(6):1886.
2.     Nawan, Isnaeni, Wasito EB. Antimicrobial activity of streptomyces sp. Isolated from acidic peatlands against extended spectrum beta lactamase (ESBL) producing escherichia coli. Res J Pharm Technol. 2020;13(3):1121-1126.
3.     Nandakumar S, Kumar MGS, Bini B, Krishnan GG. Antimicrobial activity of selected medicinal plants against oral microflora. Res J Pharm Technol. 2016;9(12):2271.
4.     Syntem LMO, Dutta H, Neelusree P, Kalyani M. Prevalence of extended spectrum ß-Lactamase and Carbapenemase producing isolates of Klebsiella SPP in a tertiary care hospital. Res J Pharm Technol. 2018;11(9):3777.
5.     Shahid SM, Umar N. Spectrum of Antimicrobial Susceptibility of E. coli and Staphylococcus aureus Isolates from Clinical Samples. Res J Pharm Technol. 2015;8(10):1399.
6.     Singh LS, Sharma H, Talukdar NC. Production of potent antimicrobial agent by actinomycete, Streptomyces sannanensis strain SU118 isolated from phoomdi in Loktak Lake of Manipur, India. BMC Microbiol. 2014;14(1):278.
7.     Zin NM, Sarmin NIM, Ghadin N, et al. Bioactive endophytic streptomycetes from the Malay Peninsula. FEMS Microbiol Lett. 2007;274(1):83-88.
8.     Bérdy J. Bioactive microbial metabolites. J Antibiot (Tokyo). 2005;58(1):1-26.
9.     Niyasom C, Boonmak S, Meesri N. Antimicrobial Activity of Acidophilic Actinomycetes Isolated from Acidic Soil. 2015;15(2):62-69.
10.     Rana S, Sirwar SB, Vijayaraghavan. Prevalence and Antibiogram of Extended Spectrum β-Lactamase Producing Klebsiella pneumoniae and Proteus mirabilis in UTI. Res J Pharm Technol. 2015;8(11):1465.
11.     de Gannes V, Bekele I, Dipchansingh D, et al. Microbial Community Structure and Function of Soil Following Ecosystem Conversion from Native Forests to Teak Plantation Forests. Front Microbiol. 2016;7:1976.
12.     Kokaz OF, Al-Dahan MAH, Sarheed NM. Molecular and Diagnostic Study of Bacteria Isolated from Human Armpit in Al Diwaniyah city. Res J Pharm Technol. 2019;12(6):2765.
13.     Karthik Raju M, Suresh Chander Kapali B, Jason Jebasingh G V., Subathra Y, Dernita Maria Nithya A. Isolation, characterization and sequencing of lactobacillus from the oral and fecal samples of healthy dogs. Res J Pharm Technol. 2018;11(11):5061-5065.
14.     Anand P, Chopra RS, Dhanjal DS, Chopra C. Isolation and characterization of microbial diversity of soil of dhanbad coal mines using molecular approach. Res J Pharm Technol. 2019;12(3):1137-1140.
15.     Srinivasan R, Karaoz U, Volegova M, et al. Use of 16S rRNA gene for identification of a broad range of clinically relevant bacterial pathogens. PLoS One. 2015;10(2):e0117617.
16.     Su G, Fu Z, Hu L, Wang Y, Zhao Z, Yang W. 16S Ribosomal Ribonucleic Acid Gene Polymerase Chain Reaction in the Diagnosis of Bloodstream Infections: A Systematic Review and Meta-Analysis. PLoS One. 2015;10(5):e0127195.
17.     Magray MSUD, Kumar A, Rawat AK, Srivastava S. Identification of Escherichia coli through analysis of 16S rRNA and 16S-23S rRNA internal transcribed spacer region sequences. Bioinformation. 2011;6(10):370-371. Accessed April 1, 2019. http://www.ncbi.nlm.nih.gov/pubmed/21904423.
18.     Clarridge JE. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin Microbiol Rev. 2004;17(4):840-862.
19.     Jo J-H, Kennedy EA, Kong HH. Research Techniques Made Simple: Bacterial 16S Ribosomal RNA Gene Sequencing in Cutaneous Research. J Invest Dermatol. 2016;136(3):e23-e27.
20.     Janda JM, Abbott SL. MINIREVIEW 16S rRNA Gene Sequencing for Bacterial Identification in the Diagnostic Laboratory : Pluses, Perils, and Pitfalls. 2007;45(9):2761-2764.
21.     Zakalyukina Y V., Zenova GM, Zvyagintsev DG. Peculiarities of growth and morphological differentiation of acidophilic and neutrophilic soil streptomycetes. Microbiology. 2004;73(1):74-78.
22.     Amutha K, Kumar AA, Godavari A. Antibacterial Effect of aqueous Neem Extract on Bacterial Isolates from Mobile Phones. Res J Pharm Technol. 2017;10(2):501.
23.     Narappanawar SN, Ranade SS, Kandpal S, Veena S, Bhaskara Rao K V. Isolation of α-amylase producing bacteria from Naini lake and assessment of their anti-oxidant and anti-fungal properties. Res J Pharm Technol. 2016;9(3):245-250.
24.     I. C. S. Evaluasi Sifat Kimia dan Fisik Gambut dari Beberapa Lokasi di Blok C Eks-Plg Kalimantan Tengah. J AGRI PEAT. 2016;17(2):67-78.
25.     Chauhan R, Singh N, Abraham J. Bioactivity and Molecular Docking of Secondary Metabolites produced by Streptomyces xanthochromogenes JAR5 . Res J Pharm Technol. 2015;8(3):300.
26.     Sankarganesh P, Joseph B. Determining the anti-malarial potential of an unknown endophytic Streptomyces species BJSG4 isolated from Kalanchoe pinnata (Lam.) pers. Res J Pharm Technol. 2019;12(9):4344-4348.
27.     Chelvan Y, Chelvan T, Pushpam AC, Karthik R, Ramalingam K, Vanitha MC. Extraction and purification of antimicrobial compounds from Marine Actinobacteria. Res J Pharm Technol. 2016;9(4):381-385.
28.     Palazzotto E, Renzone G, Fontana P, et al. Tryptophan promotes morphological and physiological differentiation in Streptomyces coelicolor. Appl Microbiol Biotechnol. 2015;99(23):10177-10189.
29.     Dhanjal DS, Chopra C, Anand P, Chopra RS. Accessing the microbial diversity of sugarcane fields from Gujjarwal village, Ludhiana and their molecular identification. Res J Pharm Technol. 2017;10(10):3439-3442.

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