Shweta Singh, Jaiganesh Rengarajan, Iyappan Sellamuthu
Shweta Singh1, Jaiganesh Rengarajan2, Iyappan Sellamuthu1*
1Department of Genetic Engineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, 603203 Kanchipuram, Chennai, Tamil Nadu, India.
2Department of Biotechnology, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, 603203 Kanchipuram, Chennai, Tamil Nadu, India.
Volume - 14,
Issue - 12,
Year - 2021
The adverse effect of pesticides used for controlling pests and the diseases caused by them is extensively rising. To overcome this harmful environmental impact, alternative methods are being studied and developed. Out of many possible methods, one effective method is to use plant extracts which embodies natural substances having antifungal properties. In an attempt towards enhancement of sustainable antifungal approach, four different concentrations of four plant extracts Citrus limon, Azadirachta indica, Ocimum gratissimum and Acalypha indica, were tested for their antifungal activity against Rhizoctonia solani, which is a serious threat for plants, and is responsible for considerable crop and yield losses. The aim of this study is to minimize yield losses and to regain the attention of formers towards exploiting natural resources for diseases control. From the results, it can be concluded that different concentrations of plant extracts have caused substantial inhibition in the mycelial growth of R. solani. The leaf extract of Ocimum gratissimum was highly effective in inhibiting mycelial growth, at the concentration of 500mg/ml of extract followed by Citrus limon, Azadirachta indica, and Acalypha indica. These plants might have potential for the development of natural fungicide for the management of diseases caused by fungal pathogens. Also, fungicides developed from these botanical extracts can prove to be highly effective for the management of plant diseases and would be simply obtainable, nonpolluting, biodegradable and economical.
Cite this article:
Shweta Singh, Jaiganesh Rengarajan, Iyappan Sellamuthu. Screening plants extracts for Antifungal activity against Rhizoctonia solani. Research Journal of Pharmacy and Technology. 2021; 14(12):6545-8. doi: 10.52711/0974-360X.2021.01132
Shweta Singh, Jaiganesh Rengarajan, Iyappan Sellamuthu. Screening plants extracts for Antifungal activity against Rhizoctonia solani. Research Journal of Pharmacy and Technology. 2021; 14(12):6545-8. doi: 10.52711/0974-360X.2021.01132 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-12-62
1. Taheri P, Gnanamanickam S, Höfte M. Characterization, Genetic Structure, and Pathogenicity of Rhizoctonia sp. Associated with Rice Sheath Diseases in India. Phytopathology. 2007; 97(3): 373-383.
2. Sturrock CJ, Woodhall J, Brown M, Walker C, Mooney SJ, Ray RV. Effects of damping-off caused by Rhizoctonia solani anastomosis group 2-1 on roots of wheat and oil seed rape quantified using X-ray Computed Tomography and real-time PCR. Frontiers in Plant Science. 2015; 6: 461.
3. Gondal AS, Rauf A, Naz, F. Anastomosis Groups of Rhizoctonia solani associated with tomato foot rot in Pothohar Region of Pakistan. Scientific Reports. 2019; 9: 3910.
4. Read PJ, Hide, G.A., Firmager, J.P. et al. Growth and yield of potatoes as affected by severity of stem canker (Rhizoctonia solani). Potato Research. 1989; 32:9–15.
5. Tsror L. Biology, epidemiology and management of Rhizoctonia solani on potato. Journal of Phytopathology. 2010; 158(10): 649–58.
6. Sid Ahmed A, Ezziyyani M, Pérez Sánchez C, Candela ME. Effect of chitin on biological control activity of Bacillus spp. and Trichoderma harzianum against root rot disease in pepper (Capsicum annuum) plants. European Journal of Plant Pathology. 2003; 109(6): 633–7.
7. Vinoth P, Subramani K, Natarajan RK, Subaiya SV, Mahesh S, Usha B and Sellamuthu I. Screening of plant growth promoting rhizobacteria for antifungal activity against Fusarium oxysporum. Journal of Environmental Biology. 2019; 40: 235-239.
8. León M, Yaryura PM, Montecchia MS, Hernández AI, Correa OS, Pucheu NL, Kerber NL, García AF. Antifungal Activity of Selected Indigenous Pseudomonas and Bacillus from the Soybean Rhizosphere. International Journal of Microbiology. 2009; 2009:1-9.
9. Ramanpreet Kaur, Mainam Anamika Devi, Pratibha Vyas. Endophytic Pseudomonas sp. TCA1 from Tinospora cordifolia Stem with Antagonistic and Plant growth-promoting Potential. Research Journal of Pharmacy and Technoloty. 2017; 10(2): 456-460.
10. Das SK, Dhake AS, Nayak A, Das NB, Pandeya SN. Antibacterial and Antifungal Activity of Aerial Part of Plant Ammannia baccifera Linn. Research Journal of Pharmacy and Technology. 2011;4(3): 430-432.
11. Jagtap NS, Khadabadi SS, Ghorpade DS, Banarase NB, Naphade SS. Antimicrobial and Antifungal Activity of Centella asiatica (L.)Urban, Umbeliferae. Research J. Pharm. and Tech.2009;2(2): 328-330.
12. Saranya Shankar, Mythili Sathiavelu. A Comparative Study on the Biological Activities of Medicinal Plants Leaf Extracts Belongs to the Family Fabaceae. Research Journal of Pharmacy and Technology.2019;12(3): 1129-1136.
13. Khuntia A, Mohanty SK. Antifungal Activity of Cleome rutidosperma Aerial Parts. Research Journal of Pharmacy and Technology. 2011;4(7): 1103-1105.
14. Nour Hassan Boissa, Maysa Yaziji, Rim Salame. Screening of antifungal activity in vitro of Cyclamen hederifolum tubers extracts. Research Journal of Pharmacy and Technology. 2016; 9(10): 1677-1680.
15. Sangavi R., Anuradha R.. Antibacterial and Antifungal Activity of Wattakaka volubilis. Research Journal of Pharmacy and Technology. 2017; 10(11): 3775-3778.
16. Ananta Choudhury, Suman Saha, Sanjib Bahadur, Amit Roy. Synergistic Antifungal Activity of Bioactive Phytochemical in Combination with Standard Antifungal Drugs. Research Journal of Pharmacy and Technology. 2019; 12(5): 2346-2352
17. Okeke MI, Okoli AS, Eze EN, Ekwume GC, Okosa EU, Iroegbu CU. Antibacterial activity of Citrus limonum fruit juice extract. Pakistan Journal of Pharmaceutical Sciences. 2015; 28(5): 1567-1571.
18. Mistry KS, Sanghvi Z, Parmar G, Shah S. The antimicrobial activity of Azadirachta indica, Mimusops elengi, Tinospora cardifolia, Ocimum sanctum and 2% chlorhexidine gluconate on common endodontic pathogens: An in vitro study. European Journal of Dentistry. 2014;8(2): 172-177.
19. Matasyoh LG, Matasyoh JC, Wachira FN, Kinyua MG, Muigai AW, Mukiama TK. Antimicrobial activity of essential oils of Ocimum gratissimum L. From different populations of Kenya. African journal of Traditional Complement Alternative Medicines. 2008; 5(2): 187-193.
20. Melo RS, Albuquerque Azevedo ÁM, Gomes Pereira AM, et al. Chemical Composition and Antimicrobial Effectiveness of Ocimum gratissimum L. Essential Oil Against Multidrug-Resistant Isolates of Staphylococcus aureus and Escherichia coli. Molecules. 2019; 24(21): 3864.
21. Govindarajan M, Jebanesan A, Reetha D, Amsath R, Pushpanathan T, Samidurai K. Antibacterial activity of Acalypha indica L. Europian Review for Medical and Pharmacological Sciences. 2008; 12(5): 299-302.
22. Noumedem JA, Tamokou Jde D, Teke GN, Momo RC, Kuete V, Kuiate JR. Phytochemical analysis, antimicrobial and radical-scavenging properties of Acalypha manniana leaves. Springerplus. 2013; 2: 503.
23. Pandey S. Antibacterial and antifungal activities of Ocimum Gratissimum L. International Journal of Pharmacy and Pharmaceutical Sciences. 2017; 9(12): 26-31.
24. Silva MR, Oliveira JG Jr, Fernandes OF, et al. Antifungal activity of Ocimum gratissimum towards dermatophytes. Mycoses. 2005; 48(3): 172-175.
25. Hayat M. Mukhtar, Mohit Mangla, Mohit Sanduja. Meticulous Approach towards Pharmacognosy and Socio-economic Impact of Ethnomedicinal Plant: Ocimum kilimandscharicum. Research Journal of Pharmacy and Technology. 2020; 13(10): 4751-4764
26. Saftarasmi, Lakshmi T. Ocimum Sanctum in Dental Care -A Mini Review. Research Journal of Pharmacy and Technology. 2014; 7(1): 101-103.
27. Otang WM, Afolayan AJ. Antimicrobial and antioxidant efficacy of Citrus limon L. peel extracts used for skin diseases by Xhosa tribe of Amathole District, Eastern Cape, South Africa. South African Journal of Botany. 2016; 102:46–49.
28. Hamdan D, Ashour ML, Mulyaningsih S, El-Shazly A, Wink M. Chemical composition of the essential oils of variegated pink-fleshed lemon (Citrus x limon L. Burm. f.) and their anti-inflammatory and antimicrobial activities. Zeitschrift für Naturforschung - Section C Journal of Biosciences. 2013; 68C: 275–284.
29. Lamine M, Rahali FZ, Hammami M, Mliki A. Correlative metabolite profiling approach to understand antioxidant and antimicrobial activities from citrus essential oils. International Journal of Food Science and Technology. 2019; 54: 2615–2623.
30. Guerrini A, Rossi D, Grandini A, Scalvenzi L, Rivera PFN, Andreotti E, Tacchini M, Spagnoletti A, Poppi I, Maietti S. Biological and chemo-diverse characterization of Amazonian (Ecuador) Citrus petitgrains. Journal of Applied Botony and Food Quality. 2014; 87: 108–116.
31. Yerima M B, Jodi S M, Oyinbo K, Maishanu H M, Farouq AA, Junaidu AU. Effect of neem extracts (Azadirachta indica) on bacteria isolated from adult mouth. Journal of Basic and Applied Sciences. 2012; 20: 64–67
32. Anjali K, Ritesh K, Sudarshan M, Jaipal SC, Kumar S. Antifungal efficacy of aqueous extracts of neem cake, karanj cake and vermicompost against some phytopathogenic fungi. The Bioscan. 2013; 8: 671–674.
33. Shrivastava DK, Swarnkar K. Antifungal activity of leaf extract of neem (Azadirachta indica Linn). International Journal of Current Microbiology and Applied Sciences. 2014; 3(5): 305–308.
34. Chekuri S, Arun Jyoti B, Sompaga SJ, Panjala S, Anupalli RR. Evaluation of AntiMicrobial and Anti-Fungal Activity of Acalyphaindica L., Leaf Extract. International Journal of Pharmacognosy and Phytochemical Research. 2018; 10(1); 48-51.