Hana R. Bajes, Sawsan A. Oran, Yasser K. Bustanji
Hana R. Bajes1*, Sawsan A. Oran2, Yasser K. Bustanji3
1Department of Science, Atlantic Cape Community College, Mays Landing, NJ 08330, USA.
1,2Department of Biological Sciences, The University of Jordan, Amman, Jordan.
3Department of Basic Medical Sciences, College of Medicine, University of Sharjah, Sharjah 27272, UAE.
3School of Pharmacy, The University of Jordan, Amman, Jordan.
Volume - 14,
Issue - 12,
Year - 2021
Alcea setosa A. (Malvaceae) is a wild plant that grows in Jordan and have several traditional medicinal uses. This study aims to collect and chemically analyze the methanolic extract from Alcea setosa A. from Jordan and to evaluate its cytotoxic and antioxidant activity against human breast cancer cells (T47D), colorectal adenocarcinoma cells (CACO2), and normal human fibroblasts (MRC5). The extract was extracted by methanol solvent and analyzed by liquid chromatography coupled with a mass spectrometer. Cell viability was assessed using trypan blue, neutral red, and MTT assays, and antioxidant activity was evaluated using DPPH scavenging activity assay. A total of 290 compounds, 12 among which were identified when compared to available standards, the extract contained six flavons derivatives, Two fatty acids, one ketone derivative, one flavonol derivative, one organic acid, and one coumarin derivative. The results also revealed that the IC50 values of the viability assays were higher among normal cells compared to the human cancer cell lines, and the viability inhibition was significant at higher concentrations compared to untreated cells. Nevertheless, moderate antioxidant activity was observed for the extract in the DPPH scavenging activity test. To sum up, this study indicates that samples of A. setosa collected from Jordan is likely to be an effective antioxidant, is optimistically potential to be utilized in breast and colon cancers treatment due to its preferential cytotoxicity against cancer cells.
Cite this article:
Hana R. Bajes, Sawsan A. Oran, Yasser K. Bustanji. Chemical Composition and Antiproliferative and Antioxidant Activities of Methanolic Extract of Alcea setosa A. Malvaceae. Research Journal of Pharmacy and Technology. 2021; 14(12):6447-4. doi: 10.52711/0974-360X.2021.01115
Hana R. Bajes, Sawsan A. Oran, Yasser K. Bustanji. Chemical Composition and Antiproliferative and Antioxidant Activities of Methanolic Extract of Alcea setosa A. Malvaceae. Research Journal of Pharmacy and Technology. 2021; 14(12):6447-4. doi: 10.52711/0974-360X.2021.01115 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-12-45
1. Al-Eisawi D, Flora of Jordan Checklist Revised. The University of Jordan Press. Amman, 2013: 149-187.
2. Oran SA, The status of medicinal plants in Jordan. Journal of Agricultural Science and Technology, 2014; A4(6A): 461-467.
3. Oran S and Al-Eisawi D, Check-list of medicinal plants in Jordan. Dirasat, 1998; 25(2): 84-112.
4. Oran SA and Al-Eisawi D, Ethnobotanical survey of the medicinal plants in the central mountains (North-South) in Jordan. Journal of Biodiversity and Environmental Sciences,2015; 6(3): 381-400.
5. Oran SA, Selected wild plant species with exotic flowers from Jordan. International Journal of Biodiversity and Conservation,2015; 7(5): 308-320.
6. Bajes HR, Almasri I, and Bustanji Y, Plant Products and Their Inhibitory Activity Against Pancreatic Lipase. Revista Brasileira de Farmacognosia, 2020; 30(3): 321-330.
7. Zeidan R, Oran S, Khleifat K, and Matar S, Antimicrobial activity of leaf and fruit extracts of Jordanian Rubus sanguineus Friv.(Rosaceae). African Journal of Microbiology Research, 2013; 7(44): 5114-5118.
8. Shehadeh MB, Sosa S, Suaifan GA, Darwish RM, Giangaspero A, Vassallo A, Lepore L, Oran SA, Hammad H, and Tubaro A, Topical anti-inflammatory potential of six Salvia species grown in Jordan. Jordan Journal of Pharmaciutical Sciences, 2014; 108(3372): 1-9.
9. Begashaw B, Mishra B, Tsegaw A. and Shewamene, Z. Methanol leaves extract Hibiscus micranthus Linn exhibited antibacterial and wound healing activities. BMC Complementary Medicine and Therapies.2017; 17, 337. https://doi.org/10.1186/s12906-017-1841-x
10. Yousef I, Oran S, Bustanji Y, Al-Eisawi D, and Irmaileh BE, Cytotoxic effect of selected wild medicinal plant species from Jordan on two different breast cancer cell lines, MCF7 and T47D. Biology and Medicine, 2018; 10(4): 1000443.
11. Abuharfeil N, Maraqa A, and Von Kleist S, Augmentation of natural killer cell activity in vitro against tumor cells by wild plants from Jordan. Journal of Ethnopharmacology,2000;71(1-2): 55-63.
12. Hatmal M, Abderrahman S, and Alsholi D, Determining the anti-tumor effects of differnt extracting methods of Arum palaestinum on different cancer cell lines by in vitro assay. Pharmacologyonline, 2017; 1: 28-45.
13. Rashan L, Hakkim FL, Fiebig HH, Kelter G, Merfort I, Al-Buloshi M, and Hasson SS, In vitro anti-proliferative activity of the Rubia tinctorum and alkanna tinctoria root extracts in panel of human tumor cell lines. Jordan Journal of Biological Sciences, 2018; 11(5): 489-494.
14. King Hussein Cancer Foundation. Local Statistics. 2020 [cited 2020 13 October]; Available from: http://www.campaigns.khcf.jo/en/section/local-statistics
15. Azab A. Alcea: Traditional medicine, current research and future opportunities. European Chemical Bulletin. 2017; 5, pp.505-14.
16. Bar-Shai N, Keasar T and Shmida A. How do solitary bees forage in patches with a fixed number of food items? Animal behaviour. 2011; 82(6), pp.1367-1372.
17. Altundag E and Ozturk M. Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia-Social and Behavioral Sciences. 2011;19, pp.756-777.
18. Said O, Khalil K, Fulder S and Azaizeh H. Ethnopharmacological survey of medicinal herbs in Israel, the Golan Heights and the West Bank region. Journal of Ethnopharmacology. 2002; 83(3), pp.251-265.
19. Jamous RM, Zaitoun SYA, Husein AI, Qasem IB and Ali-Shtayeh MS. Screening for biological activities of medicinal plants used in traditional Arabic Palestinian herbal medicine. European Journal of Medicinal Plants, 2015; pp.1-13.
20. Kaileh M, Berghe WV, Boone E, Essawi T and Haegeman G. Screening of indigenous Palestinian medicinal plants for potential anti-inflammatory and cytotoxic activity. Journal of Ethnopharmacology, 2007; 113(3), pp.510-516.
21. Alhage J and Elbitar H. In vitro screening for antioxidant and antimicrobial properties of three lebanese medicinal plants crude extracts. Pharmacognosy Research, 2019;11(2), p.127.
22. Sdayria J, Rjeibi I, Feriani A, Ncib S, Bouguerra W, Hfaiedh N, Elfeki A and Allagui M.S. Chemical composition and antioxidant, analgesic, and anti-inflammatory effects of methanolic extract of Euphorbia retusa in mice. Pain Research and Management, 2018.
23. Mustafa Abdullah Yilmaz. Simultaneous quantitative screening of 53 phytochemicals in 33 species of medicinal and aromatic plants: A detailed, robust and comprehensive LC–MS/MS method validation. Industrial Crops and Products. 2020; volume 149, 2020, https://doi.org/10.1016/j.indcrop.2020.112347
24. Al-Qudah MA, Otoom NK, Al-Jaber HI, Saleh AM, Abu Zarga MH, Afifi FU and Abu Orabi ST. New flavonol glycoside from Scabiosa prolifera L. aerial parts with in vitro antioxidant and cytotoxic activities. Natural product research, 2017; 31(24), pp.2865-2874
25. Kotha RR, Kulkarni RG, Garige AK, Nerella SG and Garlapati A. Synthesis and cytotoxic activity of new chalcones and their flavonol derivatives. Journal of Medicinal. Chemistry. 2017; 7, pp.353-360
26. Tin NNT, Truc NDT, Hang HTT, Trinh PTN, Bach LG, Dung LT. Chemical Constituents of the Aerial Parts of Scoparia dulcis and Anti-Cancer, Anti-Inflammatory Activities. KEM. 2019; 814, 360–364. https://doi.org/10.4028/www.scientific.net/kem.814.360
27. Hassan GF, Omer MA, Babadoust S and Najat DD. Flavonoids from Euphorbia condylocarpa roots. International Journal of Chemistry and Biochemistry Sciences. 2014; 6, pp.56-60.
28. Kekelidze I, Ebelashvili N, Japaridze M, Chankvetadze B Chankvetadze L. Phenolic antioxidants in red dessert wine produced with innovative technology, Annals of Agrarian Science, 2018; volume 16, Issue 1, Pages 34-38, https://doi.org/10.1016/j.aasci.2017.12.005
29. Vicente Serna-Escolano, Domingo Martínez-Romero, María J. Giménez, María Serrano, Santiago García-Martínez, Daniel Valero, Juan M. Valverde, Pedro J. Zapata, Enhancing antioxidant systems by preharvest treatments with methyl jasmonate and salicylic acid leads to maintain lemon quality during cold storage, Food Chemistry. 2021; Volume 338,128044, https://doi.org/10.1016/j.foodchem.2020.128044
30. Davis JN, Kucuk O, Djuric Z, Sarkar FH. Soy isoflavone supplementation in healthy men prevents NF-kappa B activation by TNF-alpha in blood lymphocytes. Free Radical Biology & Medicine - Journal; 2001,30:1293-302. 119
31. Alhasan SA, Pietrasczkiwicz H, Alonso MD, Ensley J, Sarkar FH. Genistein-induced cell cycle arrest and apoptosis in a head and neck squamous cell carcinoma cell line. Nutrition and Cancer 1999; 34:12-9
32. Teixeira B, Marques A, Ramos C, Batista I, Serrano C, Matos O, Neng NR, Nogueira JM, Saraiva JA, and Nunes ML, European pennyroyal (Mentha pulegium) from Portugal: Chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil. Industrial Crops and Products. 2012; 36(1): 81-87.
33. Gad SC, Alternatives to in vivo studies in toxicology, in general and applied toxicology, B. Ballantyne, T. Marrs, and T. Syversen, Editors. Grove’s dictionari’s Inc.: USA.1999; p. 178.
34. Shaheen H, Assessment of antiproliferative and antioxidant activities of the extracts of two wild plants from Jordan; Sedum nicaeense all. (Crassulaceae) and Arbutus andrachne l. (Ericaceae) in vitro, in Department of Biological Sciences. 2019, The University of Jordan.
35. Silva VR, Corrêa RS, Santos LdS, Soares MBP, Batista AA, and Bezerra DP, A ruthenium-based 5-fluorouracil complex with enhanced cytotoxicity and apoptosis induction action in HCT116 cells. Scientific Reports, 2018; 8(1): 288.
36. Bustanji Y, AlDouri N, Issa A, Mashallah S, Assaf A, Aburjai T, and Mohammad M, Cytotoxic effect of Mercurialis annua L. methanolic extract on six human solid cancer cell lines. Scientific Research Essays. 2012; 7(37): 3218-3222.
37. Jayashree V and Thenmozhi N, In-vitro anti-proliferative assay and cell viability activity of baicalein using breast cancer cell line. International Journal of Pharmaciutical Science and Research, 2018; 9(4): 1620-1624.
38. Fotakis G and Timbrell JA, In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicology Letters, 2006; 160(2): 171-177.
39. Machana S, Weerapreeyakul N, Barusrux S, Nonpunya A, Sripanidkulchai B, and Thitimetharoch T, Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2) cell line. Chinese Medicine. 2011; 6(1): 39.
40. Al-Eisawi DM, 1998. Field guide to wild flowers of Jordan and neighboring countries.
41. Althaher A, Oran S, and Bustanji Y. Phytochemical Analysis, In vitro Assessment of Antioxidant Properties and Cytotoxic Potential of Ruta chalepensis L. Essential Oil, Journal of Essential Oil Bearing Plants. 2021; 23:6, 1409-1421, DOI: 10.1080/0972060X.2020.1871078
42. Yousef I, Oran S, Alqaraleh M and Bustanji Y. Evaluation of Cytotoxic, Antioxidant and Antibacterial Activities of Origanum dayi, Salvia palaestina and Bongardia chrysogonum Plants Growing Wild in Jordan. Tropical Journal of Natural Product Research. 2021; 5(1):66-70
43. Wen SH, Su SC, Liou BH, Lin CH, and Lee KR. Sulbactam-enhanced cytotoxicity of doxorubicin in breast cancer cells. Cancer Cell International. 2018; 18: 128.
44. Li H, Krstin S, Wang S, and Wink M, Capsaicin and piperine can overcome multidrug resistance in cancer cells to doxorubicin. Molecules, 2018; 23(3): 557.
45. Rathi MA., Meenakshi P., Guru KD., Arul RC., Sunitha M., Gopalakrishnan VK. Leaves of Spermacoce hispida as a Novel Cancer Therapeutic – An In Vitro Study. Research Journal of Pharmacy and Technology. 2011; 4(8), Page 1288-1291.
46. Teeli RA., Ganie SA., Dar MS., Raja W., Yadav SS. Antioxidant activity of Euphorbia hirta L. leaf extracts. Research Journal Pharmacy and Technology. 2018; 11(1): 199-202
47. Akshay R. Yadav, Shrinivas K. Mohite. Anticancer Activity of Psidium guajava Leaf Extracts on Breast Cancer Cell Line. Resarch Journal of Pharmaceutical Dosage Forms and Technology. 2020; 12(4):298-300. doi: 10.5958/0975-4377.2020.00049.X
48. Momin MA., Rashid M., Urmi KF., Rana S. Phytochemical Screening and Investigation of Antioxidant and Cytotoxicity Potential of different extracts of selected Medicinal Plants of Bangladesh. Research Journal of Pharmacy and Technology. 2013; 6(9), Page 1042-1050.
49. NasimunIslam N., Jemimah S., Shaik SS., Kumar V., Mohanasrinivasan V., Subathra D., Panneerselvam A. Cytotoxic Property of Cocos nucifera shell Extracts on HeLa Cells. Research Journal Pharmacy and Technology. 2014; 7(5), Page 521-525.
50. Chauhan R., D’Souza H., Shabnam RS, Abraham J. Phytochemical and Cytotoxicity Analysis of Seeds and Leaves of Adenanthera pavonina. Research Journal of Pharmacy and Technology.2015; 8(2), Page 198-203. doi: 10.5958/0974-360X.2015.00036.0
51. Ahirwar B., Ahirwar D. In vivo and in vitro investigation of cytotoxic and antitumor activities of polyphenolic leaf extract of Hibiscus sabdariffa against, breast cancer cell lines. Research Journal Pharmacy and Technology. 2019; 13(2):615-620. doi: 10.5958/0974-360X.2020.00116.X
52. Chac L., Thinh B., Yen n. Anti-cancer activity of dry extract of Anoectochilus setaceus Blume against BT474 breast cancer cell line and A549 lung cancer cell line. Research Journal of Pharmacy and Technology. 2021; 14(2):730-734. doi: 10.5958/0974-360X.2021.00127.X
53. Valli G, Jeyalakshmi M. Preliminary Phytochemical and Antioxidant Study of Odina woodier Leaf Extract. Asian Journal of Pharmaciutical Research. 2012; 2(4), Page 153-155.
54. Dhundhwal DK, Senthilkumar KL, Joshua Samuel L., Senthilkumar K. Antioxidant Activity of Ethanolic and Aqueous Extracts of Leaves of Plumeria rubra (Linn.). Research Journal of Pharmacognosy and Phytochemistry 2009; 1(2): 126-127.
55. Sharma P, Jha AB, Dubey RS, Pessarakli M. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of botany. 2012; 2012, pages 1-26.