Author(s): Mangestuti Agil, Hening Laswati, Hadi Kuncoro, Burhan Ma’arif


DOI: 10.52711/0974-360X.2021.01102   

Address: Mangestuti Agil1, Hening Laswati2, Hadi Kuncoro3, Burhan Ma’arif4
1Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia.
2Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
3Faculty of Pharmacy, Mulawarman University, Samarinda, Indonesia.
4Department of Pharmacy, Faculty Medical and Health Science, Maulana Malik Ibrahim State Islamic University, Malang, Indonesia.
*Corresponding Author

Published In:   Volume - 14,      Issue - 12,     Year - 2021

Phytoestrogens are plant-derived chemical substances that have estrogen-like structures or estrogenic functions. Deficiency of estrogen in human brain causes neuroinflammation characterized by increase of major histocompatibility complex class II (MHC II) expression as a marker of M1 phenotype in microglia. Recent research found phytoestrogen compounds in Marsilea crenata Presl. The aim of this study was to investigate the effect of ethyl acetate fraction of Marsilea crenata Presl. leaf extract in MHC II expression of microglial HMC3 cell lines, for resolution of inflammation and tissue repair. The fractions were given at concentrations of 62.5, 125, and 250 ppm to microglia, that had been previously induced by IFN? 10 ng for 24 hours to stimulate the cells into M1 phenotype. Genistein as phytoestrogen was given at a concentration of 50 µM as positive control. Expression of MHC II was analyzed using immunocytochemistry method. Result showed reduction in MHC II expression of microglial cells, which indicated the activity of all extracts and, showed that 250 ppm of the fraction showed the strongest effect with MHC II value expression of 148.632 AU, and ED50 of 1,590 ppm. It was concluded from the study, that ethyl acetate fraction of Marsilea crenata Presl. leaves has antineuroinflammation effect.

Cite this article:
Mangestuti Agil, Hening Laswati, Hadi Kuncoro, Burhan Ma’arif. Effect of Ethyl Acetate Fraction of Marsilea crenata Presl. Leaf Extract on Major Histocompatibility Complex Class II Expression in Microglial HMC3 Cell Lines. Research Journal of Pharmacy and Technology. 2021; 14(12):6374-8. doi: 10.52711/0974-360X.2021.01102

Mangestuti Agil, Hening Laswati, Hadi Kuncoro, Burhan Ma’arif. Effect of Ethyl Acetate Fraction of Marsilea crenata Presl. Leaf Extract on Major Histocompatibility Complex Class II Expression in Microglial HMC3 Cell Lines. Research Journal of Pharmacy and Technology. 2021; 14(12):6374-8. doi: 10.52711/0974-360X.2021.01102   Available on:

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