Nurhabibah, A.K. Nugroho, Ronny Martien, Endang Lukitaningsih
Nurhabibah1,2, A.K. Nugroho1*, Ronny Martien1, Endang Lukitaningsih1
1Department of Pharmaceutics, Faculty of Pharmacy, University of Gadjah Mada, Sekip Utara, D.I Yogyakarta 55281, Indonesia.
2Department of Pharmacy, Faculty of Mathematics and Natural Science, University of Garut, Garut, West Java 44151, Indonesia.
Volume - 14,
Issue - 12,
Year - 2021
This study aimed to determine the solubility of lovastatin (LV) in different oil, surfactant, and co-surfactant using the high-performance liquid chromatography method. LV was solubility studies in different vehicle. The different vehicle used almond oil, sunflower oil, oleic acid, olive oil, soybean oil, and corn oil, isoprophyl myristate, myoglyol, tween 80, tween 20, and cremophor R.H. 40, propylene glycol, and PEG 400. Each of them was added lovastatin until saturated. The mixtures were mixing, sonicating, putting in the water bath and standing for 24 hours, then centrifugated. Each of the aliquot 2 µL diluted with acetonitrile and determination of concentration lovastatin using HPLC, with detector ultraviolet at 237 nm. Before determinate LV validated, and curve calibration at range 2-16 µg/mL was made. This study using the HPLC method with detector UV 237 nm, Agilent C 18 (4.6 x 150 mm 5 µ) column, and acetonitrile: water (70:30 v/v) as mobile phase. Calibration curve of lovastatin at the range 2-16 µg/mL with linear regression 0.999. Accuracy and precision showed that. Lovastatin has high soluble in oleic acid, tween 80, and PEG 400.
Cite this article:
Nurhabibah, A.K. Nugroho, Ronny Martien, Endang Lukitaningsih. Solubility Studies and Validation of Lovastatin using High Performance Liquid Chromatography Method. Research Journal of Pharmacy and Technology. 2021; 14(12):6285-8. doi: 10.52711/0974-360X.2021.01087
Nurhabibah, A.K. Nugroho, Ronny Martien, Endang Lukitaningsih. Solubility Studies and Validation of Lovastatin using High Performance Liquid Chromatography Method. Research Journal of Pharmacy and Technology. 2021; 14(12):6285-8. doi: 10.52711/0974-360X.2021.01087 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-12-17
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