In this study, two analytical methods were used to determinate the protein, the ammonia ion selective electrode method and dye binding method using orange G and the spectrophotometer at ?max 478 nm by determining the linearity, accuracy, precision, limit of detection and limit of quantitation of each. In comparison, the dye binding method was chosen for its accuracy, repeatability, sensitivity (LOD, LOQ) and speed of performance. After that, it was applied to samples of prepared plain yogurt to study effect of different properties (source, heat treatment and type) of used milk on protein content of plain yogurt.
Cite this article:
Heba Kashour, Lina Soubh. Comparative between Ammonia Ion Selective Electrode and Dye Binding Method to study effect of Processing Methods on Protein Content of Plain Yogurt. Research Journal of Pharmacy and Technology. 2021; 14(12):6257-1. doi: 10.52711/0974-360X.2021.01082
Heba Kashour, Lina Soubh. Comparative between Ammonia Ion Selective Electrode and Dye Binding Method to study effect of Processing Methods on Protein Content of Plain Yogurt. Research Journal of Pharmacy and Technology. 2021; 14(12):6257-1. doi: 10.52711/0974-360X.2021.01082 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-12-12
1. Tremblay L, Laporte MF, Leonil J, Dupont D and Paquin P. Quantitation of proteins in milk and milk products. InAdvanced Dairy Chemistry—1 Proteins. Springer, Boston, MA. 2003; pp. 49-138.
2. Tarassuk NP. The dye binding of milk proteins (No. 1369). US Department of Agriculture. 1967.
3. Sfakianakis P and Tzia C. Conventional and innovative processing of milk for yogurt manufacture. development of texture and flavor: A review. Foods. 2014; 3(1):176-193.
4. Bruhn JC, Pecore S, Franke AA. Measuring protein in frozen dairy desserts by dye binding. Journal of Food Protection. 1980; 43(10):753-755.
5. Sherbon JW. Recent developments in determining protein content of dairy products by dye binding. Journal of Dairy Science. 1978; 61(9):1274-1278.
6. Ashworth US, Seals R and Erb RE. An improved procedure for the determination of milk proteins by dye binding. Journal of Dairy Science. 1960;43(5):614-623.
7. Patel PH. Accounting for Milk Protein in Equivalents by Dye Binding Analysis of Cheese and Whey. 1969.
8. Dolby RM. Dye-binding methods for estimation of protein in milk. J. Dairy Research. 1961;28:43.
9. Buckee GK. Estimation of nitrogen with an ammonia probe. Journal of the Institute of Brewing. 1974;80(3):291-294.
10. Altemimi AB. Extraction and optimization of potato starch and its application as a stabilizer in yogurt manufacturing. Foods. 2018;7(2):14.
11. McKenzie LR and Young PN. Determination of ammonia-, nitrate-and organic nitrogen in water and waste water with an ammonia gas-sensing electrode. Analyst. 1975;100(1194):620-628.
12. Official Methods of Analysis (1995) 16th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, sec. 33.2.11, Method 991.20
13. Hilali M, El-Mayda E and Rischkowsky B. Characteristics and utilization of sheep and goat milk in the Middle East. Small Ruminant Research. 2011;101(1-3):92-101.
14. Gomes JJ, Duarte AM, Batista AS, de Figueiredo RM, de Sousa EP, de Souza EL and do Egypto RD. Physicochemical and sensory properties of fermented dairy beverages made with goat's milk, cow's milk and a mixture of the two milks. LWT-Food Science and Technology. 2013;54(1):18-24.
15. Kolar CW, Cho IC and Watrous WL. Vegetable protein application in yogurt, coffee creamers and whip toppings. Journal of the American Oil Chemists' Society. 1979;56(3Part3):389-391.
16. Belitz HD, Grosch W, Schieberle P. Milk and Dairy Products. In Food chemistry. 2009:498-545.
17. Walstra P, Walstra P, Wouters JT and Geurts TJ. Dairy Science and Technology. CRC press. 2005; ch7.
18. Guinee TP and O’Callaghan DJ. Effect of increasing the protein-to-fat ratio and reducing fat content on the chemical and physical properties of processed cheese product. Journal of Dairy Science. 2013;96(11):6830-6839.