Author(s):
Rakam Gopi Krishna, M. Srinivasa Murthy, V. Kavya
Email(s):
gopirakam@gmail.com
DOI:
10.52711/0974-360X.2021.01019 
Address:
Rakam Gopi Krishna1*, M. Srinivasa Murthy1, V. Kavya2
1Department of Pharmaceutical Analysis, Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Pochampally, Hyderabad. Telangana 508284 India.
2Department of Pharmaceutical Analysis, Chaitanya College of Pharmacy Education & Research, Hanamkonda, Warangal, Telangana 506001 India.
*Corresponding Author
Published In:
Volume - 14,
Issue - 11,
Year - 2021
ABSTRACT:
The objective of the study was to develop RP-HPLC method for the determination of purity of sumatriptan in bulk and pharmaceutical dosage form. The development of an analytical method for the determination of drugs by HPLC has received considerable attention in recent years because of their importance in quality control of drugs and drug products. The aim of this current study was to develop a simple, rapid, precise, accurate and sensitive HPLC method for the analysis of sumatriptan in bulk and its pharmaceutical dosage form by using solvent system of TEA : ACN : methanol in the ratio 80:10:10 and C8 ODS Inertsil (250*4.6mm, 5?i.d) stationary phase. The chromatographic condition is set at flow rate of 1ml/min with PDA detector at 221 nm. As per ICH requirements validation studies are carried out by using freshly prepared solutions. The linearity was demonstrated over the concentration range of 5-150 µg / ml and value was found to be as 0.99998. The %RSD of precision was found to be 0.260. The LOD and LOQ were found to be 1.967µg/ml and 5.961µg/ml respectively. Forced degradation studies were carried out under various stress conditions to demonstrate the stability-indicating capability of the developed RP-HPLC method. The proposed method was found to be simple, precise, accurate and validated according to the International Council for Harmonisation guidelines.
Cite this article:
Rakam Gopi Krishna, M. Srinivasa Murthy, V. Kavya. Method development and validation of RP-HPLC method for the determination of sumatriptan in bulk and pharmaceutical dosage form. Research Journal of Pharmacy and Technology. 2021; 14(11):5856-2. doi: 10.52711/0974-360X.2021.01019
Cite(Electronic):
Rakam Gopi Krishna, M. Srinivasa Murthy, V. Kavya. Method development and validation of RP-HPLC method for the determination of sumatriptan in bulk and pharmaceutical dosage form. Research Journal of Pharmacy and Technology. 2021; 14(11):5856-2. doi: 10.52711/0974-360X.2021.01019 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-11-44
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