Author(s):
Manu Jose, Stephin Baby, Dona Mathew, Naurin Muhammed, Jayalakshmi P M
Email(s):
manujose09@gmail.com , stephinbaby4@gmail.com , donamathew13@gmail.com , naurinmuhammed@gmail.com , pmjayalakshmi94@gmail.com
DOI:
10.52711/0974-360X.2021.01015 
Address:
Manu Jose1*, Stephin Baby2, Dona Mathew2, Naurin Muhammed2, Jayalakshmi P M3
1Assistant Professor, Nirmala College of Pharmacy Muvattupuzha-686661, Dist-Ernakulam, Kerala, India.
2Nirmala College of Pharmacy Muvattupuzha-686661, Dist-Ernakulam, Kerala, India.
3College of pharmaceutical Sciences, Gov medical college, thiruvanandapuram, Kerala.
*Corresponding Author
Published In:
Volume - 14,
Issue - 11,
Year - 2021
ABSTRACT:
The demand for herbal medicines in many pharmaceutical sectors is growing at a drastic rate due to their improved pharmacological actions, minimal side effects and cost-effectiveness. Anti-inflammatory phytochemicals are found to be efficacious against the treatment of inflammatory diseases like rheumatoid arthritis, glomerulonephritis, hepatitis, inflammatory bowel disease, etc. Anti-diabetic phytochemicals are found to treat the increasing incidence of diabetes prevalent globally. This work aims to perform the phytochemical screening and to evaluate the antidiabetic and anti-inflammatory activity of crude extract of Apama siliquosa Lamk. The method employed for obtaining the active principles includes soxhlation technique with methanol as solvent. The anti-inflammatory property was studied in vitro using inhibition of albumin denaturation technique as well as heat-induced hemolysis and IC50 value was found to be 39.5µg/ml and 36.30µg/ml respectively. The anti-diabetic activity was estimated using the alpha-amylase inhibition assay and Glucose diffusion inhibitory study. The IC50 value for alpha-amylase inhibition assay was found to be 15.75µg/ml. It also shows a strong inhibition of glucose across the dialysis membrane.
Cite this article:
Manu Jose, Stephin Baby, Dona Mathew, Naurin Muhammed, Jayalakshmi P M. Phytochemical Analysis and In-vitro Anti-Diabetic and Anti- Inflammatory study of root extract of Apama siliquosa LAMK. Research Journal of Pharmacy and Technology. 2021; 14(11):5838-2. doi: 10.52711/0974-360X.2021.01015
Cite(Electronic):
Manu Jose, Stephin Baby, Dona Mathew, Naurin Muhammed, Jayalakshmi P M. Phytochemical Analysis and In-vitro Anti-Diabetic and Anti- Inflammatory study of root extract of Apama siliquosa LAMK. Research Journal of Pharmacy and Technology. 2021; 14(11):5838-2. doi: 10.52711/0974-360X.2021.01015 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-11-40
REFERENCES:
1. Nn, Azwanida. “A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation.” Medicinal and Aromatic plants.2015;4: 1-6.
2. Patel DK, Kumar R, Prasad SK, Sairam K, Hemalatha S. Antidiabetic and in vitro antioxidant potential of Hybanthus enneaspermus (Linn) F. Muell in streptozotocin–induced diabetic rats. Asian Pac J Trop Biomed. 2011; 1(4):316–22.
3. Singh LW. Traditional medicinal plants of Manipur as anti-diabetics. J Med Plants Res. 2011; 5(5):677–87.
4. Edoga, H.O. Okwu, D.E. Mbaebie, B.O.2005 Phytochemicals constituents of some Nigerian medicinal plants. Afr. J.Biotechnol.1978;4(7):685-688
5. Almeida RN, Navarro DS, Barbosa-Filho JM. Plants with central analgesic activity. Phytomedicine. 2001;8:310–22.
6. Kummer CL, Coelho TC. Cycloxygenase-2 inhibitors nonsteroid anti-inflammatory drugs: current issues. Rev Bras Anestesiol. 2002;52:498–512.
7. Dia VP, Bringe NA, de Mejia EG. Peptides in pepsin-pancreatin hydrolysates from commercially available soy products that inhibit lipopolysaccharide-induced inflammation in macrophages. Food Chem. 2014; 152:423–31.
8. Alessandri AL, Sousa LP, Lucas CD, Rossi AG, Pinho V, Teixeira MM. Resolution of inflammation: Mechanisms and opportunity for drug development. Pharmacol Ther. 2013; 139:189–212.
9. Biswas M, Biswas K, Ghosh AK, Haldar PK. A pentacyclic triterpenoid possessing anti-inflammatory activity from the fruits of Dregea volubilis. Phcog Mag. 2009; 5:64–68.
10. Z Mary S N Yoganarasimhan J K Pattanshetty R C Nayar. Pharmacognostical studies on the root of Apama siliquosa Lamk. (Aristolochiaceae). Proc. Indian Acad. Sci. 1977; 85(6): 412-419
11. Packer, N.M. (1978), Secondary metabolism by J. Mann. Oxford Chemistry Series (No. 27). Pp. 316. Clarendon Press, Oxford. 1978. Biochem. Educ., 6: 92-92.
12. Unnikrishnan PS, Suthindhiran K, Jayasri MA. Alpha-amylase Inhibition and Antioxidant Activity of Marine Green Algae and its Possible Role in Diabetes Management. Pharmacogn Mag. 2015; 11(Suppl 4):S511-S515.
13. Vasudev Pai, Rudraprabhu V. Savadi, Anant Bhandarkar. In-Vitro Alpha-Amylase Inhibition Action of Isolated Phytoconstituent in Zanthoxylum rhetsa (Roxb) Bark. Research J. Pharm. and Tech. 4(7): July 2011; Page 1147-1150.
14. Sneha Anarthe, Sanjay Chaudhari. Hypoglycemic Activity of Leucas linifolia (Lamiaceae) Spreng. Research J. Pharmacognosy and Phytochemistry 2011; 3(1): 34-37.
15. A.M Gallagher, P.R Flatt, G Duffy, Y.H.A Abdel-Wahab, The effects of traditional antidiabetic plants on in vitro glucose diffusion, Nutrition Research.2003;23(3): 413-424.
16. T. Sundarrajan, V. Velmurugan, R.Srimathi. Phytochemical Evaluation and In Vitro Antidiabetic Activity of Ethanolic extract of Alternanthera ficodia Linn. Research J. Pharm. and Tech. 2017; 10(9): 2981-2983.
17. Mizushima Y and Kobayashi M. Interaction of anti-inflammatory drug with serum proteins especially with some biologically active proteins. Journal of Pharmacy and Pharmacology.1968;20:169-173.
18. Sakat .s. in-vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata . International journal of pharma and pharmacological science.2010;2(1) ;146-155.
19. Praneetha V, Sreerama Usha Rani, K Srinivas. Synthesis and Invitro Anti-Inflammatory activity of some 1, 2-disubstituted benzimidazoles. Asian J. Research Chem. 2016; 9(10):462-468.
20. Oyedepo, Anti protease and membrane stabilizing activities of extracts of Fagra Zanthoxiloides, Int J of Pharmacognosy. 1995; 33: 65-69.
21. Sadique J, The bioactivity of certain medicinal plants on the stabilization of RBS membrane system Fitoterapia .1989, 60:525-535.
22. Shinde UA. Mast cell stabilising and lipoxygenase inhibitory activity of Cedrus deodara (Roxb). Loud .wood oil. Indian J Exp Biol. 1999;37(3):258-261.