Author(s): Manu Jose, Stephin Baby, Dona Mathew, Naurin Muhammed, Jayalakshmi P M

Email(s): manujose09@gmail.com , stephinbaby4@gmail.com , donamathew13@gmail.com , naurinmuhammed@gmail.com , pmjayalakshmi94@gmail.com

DOI: 10.52711/0974-360X.2021.01015   

Address: Manu Jose1*, Stephin Baby2, Dona Mathew2, Naurin Muhammed2, Jayalakshmi P M3
1Assistant Professor, Nirmala College of Pharmacy Muvattupuzha-686661, Dist-Ernakulam, Kerala, India.
2Nirmala College of Pharmacy Muvattupuzha-686661, Dist-Ernakulam, Kerala, India.
3College of pharmaceutical Sciences, Gov medical college, thiruvanandapuram, Kerala.
*Corresponding Author

Published In:   Volume - 14,      Issue - 11,     Year - 2021


ABSTRACT:
The demand for herbal medicines in many pharmaceutical sectors is growing at a drastic rate due to their improved pharmacological actions, minimal side effects and cost-effectiveness. Anti-inflammatory phytochemicals are found to be efficacious against the treatment of inflammatory diseases like rheumatoid arthritis, glomerulonephritis, hepatitis, inflammatory bowel disease, etc. Anti-diabetic phytochemicals are found to treat the increasing incidence of diabetes prevalent globally. This work aims to perform the phytochemical screening and to evaluate the antidiabetic and anti-inflammatory activity of crude extract of Apama siliquosa Lamk. The method employed for obtaining the active principles includes soxhlation technique with methanol as solvent. The anti-inflammatory property was studied in vitro using inhibition of albumin denaturation technique as well as heat-induced hemolysis and IC50 value was found to be 39.5µg/ml and 36.30µg/ml respectively. The anti-diabetic activity was estimated using the alpha-amylase inhibition assay and Glucose diffusion inhibitory study. The IC50 value for alpha-amylase inhibition assay was found to be 15.75µg/ml. It also shows a strong inhibition of glucose across the dialysis membrane.


Cite this article:
Manu Jose, Stephin Baby, Dona Mathew, Naurin Muhammed, Jayalakshmi P M. Phytochemical Analysis and In-vitro Anti-Diabetic and Anti- Inflammatory study of root extract of Apama siliquosa LAMK. Research Journal of Pharmacy and Technology. 2021; 14(11):5838-2. doi: 10.52711/0974-360X.2021.01015

Cite(Electronic):
Manu Jose, Stephin Baby, Dona Mathew, Naurin Muhammed, Jayalakshmi P M. Phytochemical Analysis and In-vitro Anti-Diabetic and Anti- Inflammatory study of root extract of Apama siliquosa LAMK. Research Journal of Pharmacy and Technology. 2021; 14(11):5838-2. doi: 10.52711/0974-360X.2021.01015   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-11-40


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