Author(s): Santosh V. Gandhi, Deepak Patil, Atul A. Baravkar

Email(s): santoshvgandhi@rediffmail.com

DOI: 10.52711/0974-360X.2021.00993   

Address: Santosh V. Gandhi1*, Deepak Patil1, Atul A. Baravkar2
1AISSMS College of Pharmacy, Kennedy Road, Near R.T.O., Pune, Maharashtra, 411001, India.
2Shardabai Pawar Institute of Pharmaceutical Sciences and Research, Baramati, Pune, Maharashtra, 413115, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 11,     Year - 2021


ABSTRACT:
In present work, chemometric-assisted UV spectrophotometric methods as well as RP-HPLC method were developed for the simultaneous estimation of Ofloxacin and Tinidazole in their combined pharmaceutical dosage form. The two chemometric methods i.e. principle component regression (PCR) and partial least square regression (PLS) were successfully applied to quantify each drug in mixture using UV absorption spectra in range of 280 to 320nm at ?? of 0.5nm. Chemometric model development was done using 24 binary mixture solutions and 12 solutions were used for validation of model. The chemometric-assisted analysis does not require any prior separation step. In addition, RP-HPLC method was also developed using THERMOSIL C18 column with a mobile phase consisting ofAcetonitrile: Phosphate Buffer (85:15% v/v), flow rate of 1 ml/min and quantification was achieved using UV detector at 300 nm. The methods were successfully applied for the simultaneous determination of these drugs in synthetic mixture. The results obtained for analysis by PCR and PLS methods were compared with RP-HPLC method and a good agreement was found.


Cite this article:
Santosh V. Gandhi, Deepak Patil, Atul A. Baravkar. Comparison of Chemometric assisted UV Spectrophotometric and RP-HPLC Method for the simultaneous determination of Ofloxacin and Tinidazole in their Combined dosage form. Research Journal of Pharmacy and Technology. 2021; 14(11):5713-8. doi: 10.52711/0974-360X.2021.00993

Cite(Electronic):
Santosh V. Gandhi, Deepak Patil, Atul A. Baravkar. Comparison of Chemometric assisted UV Spectrophotometric and RP-HPLC Method for the simultaneous determination of Ofloxacin and Tinidazole in their Combined dosage form. Research Journal of Pharmacy and Technology. 2021; 14(11):5713-8. doi: 10.52711/0974-360X.2021.00993   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-11-18


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