Author(s):
Imelia Wijaya, Chrismis Novalinda Ginting, Sahna Ferdinand Ginting, Refi Ikhtiari
Email(s):
wijayaimelia4@gmail.com
DOI:
10.52711/0974-360X.2021.00989 
Address:
Imelia Wijaya, Chrismis Novalinda Ginting, Sahna Ferdinand Ginting, Refi Ikhtiari
1Department of Biomedical Science, Faculty of Medicine, Universitas Prima Indonesia, North Sumatra, Indonesia, 20118.
2Department of Clinical Pathology, Royal Prima Hospital, North Sumatra, Indonesia, 20118.
*Corresponding Author
Published In:
Volume - 14,
Issue - 11,
Year - 2021
ABSTRACT:
This study reports the bioactive compound of black cumin honey and investigates the antibacterial activity against Propionibacterium acnes and Pseudomonas aeruginosa. HPLC and GC-MS methods were used to analyze the bioactive compounds of the black cumin honey. The formulated cream of black cumin honey was prepared in an oil-in-water type cream with various concentrations of 1%, 5%, 10%, 15%, 20%, and 30% (w/w). Antibacterial effects against Propionibacterium acnes and Pseudomonas aeruginosa were examined by the disc diffusion method. Physical stability of formulated cream included organoleptic, homogeneity, and pH measurement test. The result of GC-MS analysis of black cumin honey showed 25 compounds: organic fatty acids, saccharides, and amino acid. HPLC analysis of flavonoid content showed quercetin as the bioactive compound at a concentration of 0.223 mg/g. Disc diffusion analysis showed great inhibitory potential against P. acnes and P. aeruginosa in 30% concentration with d = 11.2 mm and 10.8 mm, respectively. It can be concluded that the formulated cream of black cumin honey showed a great potential application as antibacterial agents of P. acnes and P. aeruginosa.
Cite this article:
Imelia Wijaya, Chrismis Novalinda Ginting, Sahna Ferdinand Ginting, Refi Ikhtiari. Phytochemical and Antibacterial Analysis of the Formulated Cream of Black Cumin Honey. Research Journal of Pharmacy and Technology. 2021; 14(11):5691-5. doi: 10.52711/0974-360X.2021.00989
Cite(Electronic):
Imelia Wijaya, Chrismis Novalinda Ginting, Sahna Ferdinand Ginting, Refi Ikhtiari. Phytochemical and Antibacterial Analysis of the Formulated Cream of Black Cumin Honey. Research Journal of Pharmacy and Technology. 2021; 14(11):5691-5. doi: 10.52711/0974-360X.2021.00989 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-11-14
REFERENCES:
1. Jibril FI, Bakar A, Hilmi M, Manivannan L. Isolation and characterization of polyphenols in natural honey for the treatment of human diseases. Bull Natl Res Cent [Internet]. 2019; 7(43:4): 9.
2. Samarghandian S, Farkhondeh T, Samini F. Honey and Health : A Review of Recent Clinical Research. Pharmacogn Res. 2017; 9: 121–7.
3. Ayoub S, Al-asiri SA, Latief A. Saudi Journal of Biological Sciences Role of honey in Modern Medicine. Saudi J Biol Sci [Internet]. 2017; 24(5): 975–8.
4. Ahmed S, Sulaiman SA, Baig AA, Ibrahim M, Liaqat S, Fatima S, et al. Review Article Honey as a Potential Natural Antioxidant Medicine : An Insight into Its Molecular Mechanisms of Action. Oxid Med Cell Longev [Internet]. 2018; 19.
5. Yimer EM, Tuem KB, Karim A, Ur-rehman N, Anwar F. Nigella sativa L . (Black Cumin): A Promising Natural Remedy for Wide Range of Illnesses. Evidence-Based Complement Altern Med [Internet]. 2019:16.
6. Shafiq H, Ahmad A, Masud T, Kaleem M. Cardio-protective and anti-cancer therapeutic potential of Nigella sativa. Iran J Basic Med Sci. 2014; 1(7): 967–79.
7. Tan HT, Rahman RA, Gan SH, Halim AS, Hassan SA, Sulaiman SA, et al. The antibacterial properties of Malaysian tualang honey against wound and enteric microorganisms in comparison to manuka honey. BMC Complement Altern Med. 2009; 9(34):1–8.
8. Stagos D, Soulitsiotis N, Tsadila C, Papaeconomou S, Arvanitis C, Ntontos A, et al. Antibacterial and antioxidant activity of different types of honey derived from Mount Olympus in Greece. Int J Mol Med. 2018; 4(2): 726–34.
9. Turgay O, Toroglu S. Antimicrobial Activity of Black Cumin Seed ( Nigella sativa L . Antimicrobial Activity of Black Cumin Seeds ( Nigella sativa L .). Asian J Chem. 2016; 21(1): 467–70.
10. Djakaria SA, Batubara I, Raffiudinc R. Antioxidant and Antibacterial Activity of Selected Indonesian Honey against Bacteria of Acne H ). J Kim Sains dan Apl [Internet]. 2020; 23(8): 267–75.
11. Chaerunisaa AY, Muhaimin M, Susilawati Y, Milanda T. Formulation of Creams Containing Active Fraction of Cassia fistula L . Barks and its Antibacterial Activity Against Propionibacterium acnes and Pseudomonas aeruginosa. Pharmacogn J. 2020;12(4):920–8.
12. Brook I, Frazier EH. Infections Caused by Propionibacterium Species. Rev Infect Dis. 1991; 13(5): 819–22.
13. Mollerup S, Friis-nielsen J, Vinner L, Hansen A, Richter R, Fridholm H, et al. Propionibacterium acnes : Disease-Causing Agent or Common Contaminant ? Detection in Diverse Patient Samples by Next- Generation Sequencing. J Clin Microbiol. 2016; 54(4): 980–7.
14. Angie E, Abdullah H, Martinus AR, Ikhtiari R. The Potential of Forest Honeybee on Decreasing Malondialdehyde Levels in Adult with Mild Acne Vulgaris. Proc Int Conf Heal Informatics Med Appl Technol (ICHIMAT 2019). 2019; 309–16.
15. Klockgether J, Tümmler B. Recent advances in understanding Pseudomonas aeruginosa as a pathogen. F1000 Research. 2017; 6.
16. Apriani EF, Nurleni N, Nugrahani HN. Stability testing of Azelaic acid cream based ethosome. Asian J Pharm Clin Res [Internet]. 2018; 11(5): 270–3.
17. Madaan V, Chanana A, Kataria MK, Bilandi A. Emulsion Technology and Recent Trends in Emulsion Applications. Int Res J Pharm. 2014;5(7):533–42.
18. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity : A review. J Pharm Anal [Internet]. 2016; 6(2): 71–9.
19. Ms NS, Gan SH, As H, Syazana N, Shah M, Gan SH, et al. Honey using gas chromatography mass spectrometry Reconstructive Sciences Unit , School of Medical Sciences , Health Campus , Universiti Sains Malaysia ,. Afr J Tradit Complement Altern Med [Internet]. 2013; 10(2): 180–8.
20. Lachman J, Hejtmánková A, Sýkora J, Karban J. Contents of Major Phenolic and Flavonoid Antioxidants in Selected Czech Honey. Czech J Food Sci. 2010; 28(5): 412–26.
21. Gad HA, El-ahmady SH. Industrial Crops and Products Prediction of thymoquinone content in black seed oil using multivariate analysis : An e ffi cient model for its quality assessment. Ind Crop Prod [Internet]. 2018; 124(8): 626–32.
22. Zhang Y, Wu Y-T, Zheng W, Han X-X, Jiang Y-H, Hu P-L, et al. The antibacterial activity and antibacterial mechanism of a polysaccharide from Cordyceps cicadae. J Funct Foods. 2017; 38: 273–9.
23. Jumina J, Mutmainah M, Kurniawan BP, Kurniawan SY, Syah YM. Antibacterial and Antifungal Activity of Three Monosaccharide Monomyristate Derivatives. Molecules. 2019; 24(3692): 1–9.
24. Jeon Y-J, Kim S-K. Effect of Antimicrobial Activity by Chitosan Oligosaccharide N-Conjugated with Asparagine.pdf. J Microbiol Biotechnol. 2001;11(2):281–6.
25. Mcgaw LJ, Jäger AK, Staden J Van. Antibacterial effects of fatty acids and related compounds from plants. South African Journal of Bot. 2002; 68: 417–23.
26. Kabara JONJ, Swieczkowski DM, Conley AJ, Truant JP. Fatty Acids and Derivatives as Antimicrobial Agents. Antimicrob Agents Chemother. 1972; 2(1): 23–8.
27. Yoon BK, Jackman JA, Valle-gonz ER, Cho N. Antibacterial Free Fatty Acids and Monoglycerides : Biological Activities , Experimental Testing , and Therapeutic Applications. Int J Mol Sci. 2018; 19(1114).
28. Choi J-S, Park N-H, Hwang S-Y, Sohn JH. The antibacterial activity of various saturated and unsaturated fatty acids against several oral pathogens. J Environ Biol. 2013;34(4):673–6.
29. Al-noor TH, Ghanim FH, Shahoobi BA. Synthesis , Physico -Chemical and Antimicrobial Properties of n (II), Fe (II), Co (II), Ni (II), Cu (II), Zn (II) and Cd (II), Mixed Ligand Complexes of cephalexin mono hydrate (antibiotics) and Furan-2-carboxylic acid. Trans Eng Sci. 2015; 3(2): 1–8.
30. Hismiogullari SE, E E, Hismiogullari AA, Sahin F, Başalan M, Yenice S meryem. Effects of Caproic and Caprylic Acids on Microbial Growth and Cytotoxicity. J Anim Vet Adv. 2008; 7.
31. Aziz NH, Farag SE, Mousa LA, Abo-Zaid MA. Comparative antibacterial and antifungal effects of some phenolic compounds. Microbios. 1998; 93(374): 43–54.
32. Choi WH, Jiang MH. Evaluation of antibacterial activity of hexanedioic acid isolated from Hermetia illucens larvae. J Appl Biomed. 2014; 12: 179–89.
33. Das A, Datta S, Mukherjee S, Bose S, Ghosh S, Dhar P. Evaluation of Antioxidative, antibacterial and probiotic growth stimulatory activities of Sesamum indicum honey containing phenolic compounds and Lignans. LWT-Food Sci Technol. 2014; 61 :244–50.
34. Jaisinghani RN. Antibacterial properties of quercetin. Microbiol Res (Pavia). 2017;8(6877):13–4.
35. Adamczak A, O ˙zarowski M, Karpi'nski TM. Antibacterial Activity of Some Flavonoids and Organic Acids Widely Distributed in Plants. J Clin Med. 2020; 9(109): 1–17.
36. Xie Y, Yang W, Tang F, Chen X, Ren L. Antibacterial Activities of Flavonoids : Structure-Activity Relationship and Antibacterial Activities of Flavonoids : Structure-Activity Relationship and Mechanism. Curr Med Chem. 2015; 22: 132–49.
37. Qiong W. Antimicrobial effect of Manuka honey and Kanuka honey alone and in combination with the bioactives against the growth of Propionibacterium acnes ATCC 6919. Massey University; 2011.
38. Albaridi NA. Antibacterial Potency of Honey. Int J Microbiol [Internet]. 2019; 1–10.
39. Molan PC. Potential of Honey in the Treatment of Wounds and Burns. Am J Clin Dermatol. 2001; 2(1): 13–9.
40. Eady EA, Layton AM, Cove JH. A Honey Trap for the Treatment of Acne : Manipulating the Follicular A Honey Trap for the Treatment of Acne : Manipulating the Follicular Microenvironment to Control Propionibacterium acnes. Biomed Res Int [Internet]. 2013; 8.
41. Mandal MD, Mandal S. Honey : its medicinal property and antibacterial activity. Asian Pac J Trop Biomed. 2011; 1(2): 154–60.
42. Olaitan PB, Adeleke OE. Honey : a reservoir for microorganisms and an inhibitory. Afr Health Sci. 2007; 7: 159–65.
43. Akhilesh Gupta, Vimal Yadav, Jaydeep S. Yadav, Swati Rawat. An Analytical Approach of Doxofylline: A Review. Asian J. Pharm. Ana. 1(4): Oct. - Dec. 2011; Page 67-70.
44. Sharmin Reza Chowdhury, Mahfuza Maleque, Mahbubul Hoque Shihan. Development and Validation of a Simple RP-HPLC Method for Determination of Caffeine in Pharmaceutical Dosage Forms. Asian J. Pharm. Ana. 2(1): Jan.-Mar. 2012; Page 01-04.
45. Mariyappan M., Bharathidasan R., Mahalingam R., Madhanraj P., Panneerselvam A., Ambikapathy V.. Antibacterial Activity of Cardiospermum halicacabum and Melothria heterophylla. Asian J. Pharm. Res. 1(4): Oct. - Dec. 2011; Page 111-113.