Author(s): Veena Devi Singh, Vijay Kumar Singh, Sanjay J Daharwal

Email(s): veena1806@gmail.com , vijaysingh@galgotiasuniversity.edu.in

DOI: 10.52711/0974-360X.2021.00965   

Address: Veena Devi Singh1*, Vijay Kumar Singh2 , Sanjay J Daharwal3
1Assistant Professor, Pharmacy Institute, Noida Institute of Engineering and Technology, Greater Noida, Uttar Pradesh, India- 201306.
2Associate Professor, School of Medical and Allied Sciences, Galgotias University, Greater Noida, Uttar Pradesh, India.
3Associate Professor, University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India -492010.
*Corresponding Author

Published In:   Volume - 14,      Issue - 10,     Year - 2021


ABSTRACT:
New HPTLC method was developed and optimized for estimation of Ondansetron (OND), Dexamethasone (DEX) and Aprepitant (APT) in laboratory prepared ternary mixtures by using Central composite design (CCD). The independent variables used for the optimization were the acetone content in mobile phase (%mL), distance of developing solvent (cm) and saturation time (min). HPTLC Separation was performed on Precoated silica gel F254 aluminum plate (10X10 cm, 100µm thickness) with a mobile phase consisting of chloroform: methanol: acetone: ethyl acetate: ammonia (9:4:2:5:0.2 % v/v/v/v). Quantification of OND, APT and DEX were achieved based on a Densitometric analysis over the concentration range of 200-1200 ng/band, 500-1000 ng/band and 1000-2000 ng/band, respectively, at 254nm. The method was yielded dense and well-resolved bands at Rf values of 0.54± 0.02, 0.79±0.02 and 0.23±0.01 for OND, APT and DEX, respectively. The linear regression analysis for the calibration plots produced r2= 0.9997, r2= 0.9998 and r2=0.9997 for OND, APT and DEX, respectively. The method was validated according to the ICH guidelines. The robustness test was determined that the selected factors have an insignificant effect on the responses. The results indicated that the method is suitable for the routine quality control testing of OND, APT and DEX in their bulk form.


Cite this article:
Veena Devi Singh, Vijay Kumar Singh, Sanjay J Daharwal. Application of Central Composite Design for screening and Optimization of HPTLC method for simultaneous quantitation of Aprepitant, Dexamethasone and Ondansetron in their synthetic mixtures. Research Journal of Pharmacy and Technology 2021; 14(10):5531-9. doi: 10.52711/0974-360X.2021.00965

Cite(Electronic):
Veena Devi Singh, Vijay Kumar Singh, Sanjay J Daharwal. Application of Central Composite Design for screening and Optimization of HPTLC method for simultaneous quantitation of Aprepitant, Dexamethasone and Ondansetron in their synthetic mixtures. Research Journal of Pharmacy and Technology 2021; 14(10):5531-9. doi: 10.52711/0974-360X.2021.00965   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-10-83


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