Author(s): Avinash Birajdar, Varsha Tegeli, Suyash Ingale, Gajanand Nangare

Email(s): avibirajdar1998@gmail.com

DOI: 10.52711/0974-360X.2021.00951   

Address: Avinash Birajdar*, Varsha Tegeli, Suyash Ingale, Gajanand Nangare
D.S.T.S. Mandal’s College of Pharmacy, Solapur-413004, Maharashtra, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 10,     Year - 2021


ABSTRACT:
A Reverse Phase High Performance Liquid Chromatographic method has been developed and validated for estimation of Ritonavir in API and Tablet formulation. The chromatographic separation was carried out using ZOROBAX Bonus-RP C-18 column (250 x 4.6mm, 5µm) as stationary phase and Methanol: Acetonitrile: 0.1% Trifluoroacetic acid water (81:9:10) as mobile phase at 1.0 ml/min flow rate and detection was carried out at 250 nm. The method was validated accordance to the Guidelines of International Council for Harmonization (ICH). Ritonavir have linearity in the concentration range of 50-150µg/ml with correlation coefficient (r2=1) respectively. Ritonavir eluted at 3.05 min respectively. The method is sensitive, precise and accurate. So, the method can be successfully applied for the routine analysis of Ritonavir in pharmaceutical formulations.


Cite this article:
Avinash Birajdar, Varsha Tegeli, Suyash Ingale, Gajanand Nangare. Development and Validation of RP-HPLC method for the Estimation of Ritonavir in API and tablet Formulation. Research Journal of Pharmacy and Technology 2021; 14(10):5457-0. doi: 10.52711/0974-360X.2021.00951

Cite(Electronic):
Avinash Birajdar, Varsha Tegeli, Suyash Ingale, Gajanand Nangare. Development and Validation of RP-HPLC method for the Estimation of Ritonavir in API and tablet Formulation. Research Journal of Pharmacy and Technology 2021; 14(10):5457-0. doi: 10.52711/0974-360X.2021.00951   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-10-69


REFERENCES:
1.    Indian Pharmacopoeia, vol. III, 2007, 1058.
2.    United States Pharmacopoeia 30, National Formulary 25, 2007, 3143.
3.    www.rxlist.com
4.    Richard M, Hoetelmans W, Marjolijn Van Essenberg, Monique Profijt, Pieter L. Meenhorst, Jan W. Mulder. High-Performance Liquid Chromatographic determination of Ritonavir in human plasma, cerebrospinal fluid and saliva. Journal of Chromatography B. 1998;705(1):119-126.
5.    Rebiere Herve, Mazel Bernard, Civade Corinne, Bonnet Pierre-Antoine. Determination of 19 antiretroviral agents in pharmaceuticals or suspected products with two methods using High-Performance Liquid Chromatography. Journal of chromatography B. 2007;850:376-383.
6.    Yekkala RS, Ashenafi D, Marien I, Xin H, Haghedooren E, Hoogmartens J et al. Evaluation of an International Pharmacopoeia method for the analysis of Ritonavir by Liquid Chromatography. Journal of pharmaceutical and biomedical analysis 2008; 48(3):1050-4.
7.    Veronica Albert, Pilar Modamio, Cecilia FL and Eduardo LM. Determination of Saquinavir and Ritonavir in human plasma by RP-HPLC and the analytical error function. Journal of Pharmaceutical and Biomedical Analysis. 2004;36(4):835-840.
8.    Usami Yoshiko, Tsuyoshi OK, Naka Masahiko, Sagisaka Masafumi, Kaneda Tsuguhiro. A simple HPLC method for simultaneous determination of Lopinavir, Ritonavir and Efavirenz. Journal-Chemical and pharmaceutical bulletin. 2003;51:715-718.
9.    Dias CL, Rossi RC, Donato EM, Bergold AM and Froehlich PE. LC Determination of Ritonavir, a HIV Protease Inhibitor, in Soft Gelatin Capsules. J chromatographia. 2005; 62:589-593.
10.    Proust V, Toth K, Hulin A, Taburet AM, Gimenez F, Singlas E. Simultaneous High-Performance Liquid Chromatographic determination of the antiretroviral agents’ Amprenavir, Nelfinavir, Ritonavir, Saquinavir, Delavirdine and Efavirenz in human plasma. Journal of chromatography B. 2000;742:453-458.
11.    K.Chiranjeevi, K.P.Channabasavaraj, P.Srinivas Reddy, P.T.Nagaraju. Development and Validation of Spectrophotometric Method for Quantitative estimation of Ritonavir in Bulk and Pharmaceutical Dosage Forms. International Journal of Chemtech Research. 2011;3(1):58- 62.
12.    K. Chiranjeevi, G. Vijaya Kumar, K.P. Channabasavaraj, Y. Manjunath. Third Order Derivative Spectrophotometric Estimation Of Ritonavir In Bulk And Pharmaceutical Dosage Forms. Int.J.Ph.Sci. 2011; 3(1):1017-1020.
13.    Dias CL, Bergold AM, Pedro EF. UV-Derivative Spectrophotometric Determination of Ritonavir Capsules and Comparison with LC Method. Anal Letrs. 2009; 42(12):1900-1910.
14.    Vaishali PN, Kishore PB. Simultaneous Estimation of Ritonavir and Lopinavir by Absorption ratio (Q-analysis) UV Spectrophotometric Method in Combined Tablet Dosage Form. Der Pharmacia Lettre. 2010;2(1):196-200.
15.    Temghare GA, Shetye SS, Joshi SS. Rapid and Sensitive Method for Quantitative Determination of Lopinavir and Ritonavir in Human Plasma by Liquid Chromatography Mass Spectrometry. E-Journal of Chemistry 2009;6(1):223-230.
16.    Yadav PK, Chandanam S, Rao ST, Akkamma HG, Bharadwajan RG, Deepa MS, New analytical method development and validation of acyclovir by RP-HPLC. Unique J Pharmaceutical and Biological Sciences. 2016;04(02):20-26.
17.    Sulebhavikar AV, Pawar UD, Mangoankar KV, Prabhunavelkar ND. HPTLC Method for Simultaneous Determination of Lopinavir and Ritonavir in Capsule Dosage Form. E-Journal of Chemistry 2008;5(4):706-712.

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